Source Paper
Chronic Stress Induces Impairment of Spatial Working Memory Because of Prefrontal Dopaminergic Dysfunction
Kazushige Mizoguchi, Mitsutoshi Yuzurihara, Atsushi Ishige, Hiroshi Sasaki, De-Hua Chui et al.
Journal of Neuroscience • 2000
T-Maze Spatial Working Memory Task
Objective: Assessment of spatial working memory performance in chronically stressed rats using a T-maze task to evaluate the relationship between chronic stress-induced working memory impairment and dopaminergic dysfunction in the prefrontal cortex
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Protocol Steps
Chronic stress administration
Rats subjected to chronic stress through water immersion and restraint procedures
Note: Stress protocol previously reported to induce organic disorder such as hippocampal neuronal degeneration
View evidence from paper
“chronic stress administered by water immersion and restraint for 4 weeks induces a organic disorder such as hippocampal neuronal degeneration”
Recovery period
Chronically stressed rats allowed to recover before behavioral testing
Note: Recovery period follows the 4-week stress protocol
View evidence from paper
“chronically stressed (4 weeks) and recovered (10 d) rats show a working memory impairment”
T-maze spatial working memory task
Rats tested on T-maze task to evaluate spatial working memory performance
Note: Task measures working memory impairment in stressed versus control rats
View evidence from paper
“The stress impaired the spatial working memory evaluated by T-maze task”
Intra-PFC infusion of SKF 81297
D1 receptor-specific agonist infused directly into prefrontal cortex to test amelioration of memory impairment
Note: 10 ng dose used; this treatment sufficiently ameliorated memory impairment
View evidence from paper
“memory impairment was sufficiently ameliorated by intra-PFC infusion of 10 ng SKF 81297, a D1 receptor-specific agonist”
Pretreatment with SCH 23390
D1 receptor antagonist administered via intraperitoneal injection prior to SKF 81297 treatment to test receptor specificity
Note: 20 μg/kg dose; pretreatment reversed the ameliorative response to SKF 81297
View evidence from paper
“Pretreatment with intraperitoneal injection of 20 μg/kg SCH 23390, a D1 receptor antagonist, reversed the SKF 81297 response”