Source Paper
Behavioral effects of a chronic envy-like stress paradigm in mice using an adjacent cage model
Ueno H, Tanaka Y, Kitano E, Takahashi Y, Mori S et al.
IBRO Neurosci Rep • 2026
Tail-suspension test
Objective: To evaluate depressive-like behavior in mice
This is a Tail-suspension test protocol using Mice as the model organism. The procedure involves 4 procedural steps, 2 equipment items, 2 materials. Extracted from a 2026 paper published in IBRO Neurosci Rep.
Model and subjects
Mice • C57BL/6 N • Male • Eight-week-old • 10
Study window
~6 minutes hands-on
Core workflow
Position chamber • Suspend mouse • Record behavior
Primary readouts
- Immobility time (s) - defined as the absence of active movement for at least 1 s, with only passive swinging permitted
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Position chamber
Place white plastic chamber 60 cm above the floor
View evidence from paper
“white plastic chamber positioned 60 cm above the floor”
Suspend mouse
Suspend mice by the tail using adhesive tape placed less than 1 cm from the tip of the tail
View evidence from paper
“Mice were suspended by the tail in a white plastic chamber positioned 60 cm above the floor, secured with adhesive tape placed less than 1 cm from the tip of the tail”
Record behavior
Record behavior for 6 minutes using a video camera
View evidence from paper
“Behavior was recorded for 6 min using a video camera”
Test frequency
Test each mouse once
View evidence from paper
“Each mouse was tested once”