Source Paper
Auditory Thalamus, Dorsal Hippocampus, Basolateral Amygdala, and Perirhinal Cortex Role in the Consolidation of Conditioned Freezing to Context and to Acoustic Conditioned Stimulus in the Rat
Benedetto Sacchetti, Carlo Ambrogi Lorenzini, Elisabetta Baldi, Giovanna Tassoni, Corrado Bucherelli
Journal of Neuroscience • 1999
Tetrodotoxin Inactivation with Consolidation Testing
Objective: Assess the role of auditory thalamus, dorsal hippocampus, basolateral amygdala, and perirhinal cortex in fear memory consolidation using reversible tetrodotoxin inactivation at varying post-acquisition delays
This is a Tetrodotoxin Inactivation with Consolidation Testing protocol using rat as the model organism. The procedure involves 5 procedural steps, 2 equipment items, 2 materials. Extracted from a 1999 paper published in Journal of Neuroscience.
Model and subjects
rat • Wistar • male • adult
Study window
~1.1 week study window | ~1.5 hours hands-on
Core workflow
Fear conditioning training • Animal anesthesia • Stereotaxic TTX injection
Primary readouts
- Context freezing duration
- CS (conditioned stimulus) freezing duration
- Consolidation disruption or preservation for each brain structure
Key equipment and reagents
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Fear conditioning training
Adult male Wistar rats underwent CS and context fear training
Note: Training must be completed before TTX injection
View evidence from paper
“adult male Wistar rats having undergone CS and context fear training”
Animal anesthesia
Anesthetize animals prior to stereotaxic injection
Note: Anesthesia type and dosage not specified in text
View evidence from paper
“Anesthetized animals were injected stereotaxically with TTX”
Stereotaxic TTX injection
Inject tetrodotoxin into target brain structures (auditory thalamus, dorsal hippocampus, basolateral amygdala, or perirhinal cortex) at varying post-acquisition delays
Note: Injection volume and dose adjusted according to site dimensions. Delays tested: early (up to 1.5 hr for DH), 2 days (BLA), and 8 days (PC)
View evidence from paper
“Anesthetized animals were injected stereotaxically with TTX (either 5 or 10 ng in 0.5 or 1.0 µl of saline, according to site dimensions) at increasing post-acquisition delays”
Retention testing at 48 hours post-injection
Measure context and CS freezing durations during retention testing
Note: First retention test timepoint
View evidence from paper
“Context and CS freezing durations were measured during retention testing, always performed 48 and 72 hr after TTX administration”
Retention testing at 72 hours post-injection
Measure context and CS freezing durations during retention testing
Note: Second retention test timepoint
View evidence from paper
“Context and CS freezing durations were measured during retention testing, always performed 48 and 72 hr after TTX administration”