Two-Way Active Avoidance
Objective: A conditioned learning task measuring active avoidance responses in rats to assess fear-related behavior and genetic influences on anxiety-related traits
This is a Two-Way Active Avoidance protocol using rat as the model organism. The procedure involves 6 procedural steps, 4 equipment items. Extracted from a 2002 paper published in Genome Research.
Model and subjects
rat • Roman high and low avoidance rats (F2 intercross) • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Conduct two-way active avoidance task • Conduct conditioned fear test • Conduct elevated plus maze test
Primary readouts
- Two-way active avoidance responses
- Conditioned fear behavior
- Elevated plus maze performance
- Open field activity
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Conduct two-way active avoidance task
Administer two-way active avoidance test to measure conditioned learning and active avoidance responses in rats
Note: Part of a battery of animal models of anxiety testing
View evidence from paper
“The QTL influences two-way active avoidance, conditioned fear, elevated plus maze, and open field activity”
Conduct conditioned fear test
Administer conditioned fear test as part of the anxiety assessment battery
Note: Measures fear-related behavior
View evidence from paper
“The QTL influences two-way active avoidance, conditioned fear, elevated plus maze, and open field activity”
Conduct elevated plus maze test
Administer elevated plus maze test to measure anxiety-related behavior
Note: Standard model of anxiety in rodents
View evidence from paper
“The QTL influences two-way active avoidance, conditioned fear, elevated plus maze, and open field activity”
Conduct open field activity test
Measure open field activity as an indicator of anxiety-related behavior
Note: Assesses locomotor activity and anxiety in novel environment
View evidence from paper
“The QTL influences two-way active avoidance, conditioned fear, elevated plus maze, and open field activity”
Conduct acoustic startle response test
Measure acoustic startle responses to acoustic stimuli
Note: This measure was not influenced by the QTL identified
View evidence from paper
“but not acoustic startle response or defecation in a novel environment”
Measure defecation in novel environment
Assess defecation as a measure of anxiety in a novel environment
Note: This measure was not influenced by the QTL identified
View evidence from paper
“but not acoustic startle response or defecation in a novel environment”