Unilateral Contralateral Lesion Study
Objective: Examine functional interactions between brain regions using unilateral hippocampal lesions combined with contralateral perirhinal or prefrontal cortex lesions to determine when the hippocampus is involved in recognition memory
This is a Unilateral Contralateral Lesion Study protocol using rat as the model organism. The procedure involves 4 procedural steps, 1 materials. Extracted from a 2011 paper published in Journal of Neuroscience.
Model and subjects
rat • Not specified • Not specified • Not specified • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Surgical preparation of lesions • Behavioral testing on object recognition memory tasks • Assessment of object-in-place memory
Primary readouts
- Object-in-place recognition memory performance
- Recency recognition memory performance
- Functional interaction between hippocampus and perirhinal cortex
- Functional interaction between hippocampus and medial prefrontal cortex
Key equipment and reagents
Verified items
0
Direct vendor links
0
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Surgical preparation of lesions
Prepare groups of rats with unilateral cytotoxic lesion in the hippocampus combined with a lesion in either the contralateral perirhinal or prefrontal cortex
Note: Lesions are unilateral in hippocampus and contralateral in perirhinal or prefrontal cortex
View evidence from paper
“groups of rats were prepared with a unilateral cytotoxic lesion in the hippocampus combined with a lesion in either the contralateral perirhinal or prefrontal cortex”
Behavioral testing on object recognition memory tasks
Test rats in a series of object recognition memory tasks following lesion preparation
Note: Tasks assess different types of recognition memory information
View evidence from paper
“Rats were then tested in a series of object recognition memory tasks”
Assessment of object-in-place memory
Test recognition memory requiring animals to use object-place information
Note: Part of battery of spontaneous object recognition tasks
View evidence from paper
“object-in-place memory, or recency information (temporal order memory)”
Assessment of recency recognition memory
Test recognition memory requiring animals to use recency information and temporal order
Note: Part of battery of spontaneous object recognition tasks
View evidence from paper
“recency information (temporal order memory)”