Visual Analogue Scale Assessment
Objective: To assess appetite sensations and palatability of test meals using visual analogue scales throughout an experiment examining the effect of GLP-1 infusion on satiety and energy intake
This is a Visual Analogue Scale Assessment protocol using human as the model organism. The procedure involves 6 procedural steps, 3 equipment items, 4 materials. Extracted from a 1998 paper published in Journal of Clinical Investigation.
Model and subjects
human • male • young • normal-weight • 20
Study window
Estimated timing pending
Core workflow
Study design setup • Initiate infusion and test meal • Assess appetite sensations with visual analogue scales
Primary readouts
- Subjective appetite sensations (satiety and fullness) measured by visual analogue scales
- Palatability of test meals measured by visual analogue scales
- Spontaneous energy intake at ad libitum lunch
- Plasma GLP-1 levels
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Study design setup
Conduct a placebo-controlled, randomized, blinded, crossover study with 20 young, healthy, normal-weight men
Note: Study is randomized, blinded, and uses a crossover design
View evidence from paper
“20 young, healthy, normal-weight men participated in a placebo-controlled, randomized, blinded, crossover study”
Initiate infusion and test meal
Start intravenous infusion of either GLP-1 (50 pmol/kg.h) or saline simultaneously with initiation of the energy-fixed breakfast test meal
Note: Infusion and meal initiation must be simultaneous
View evidence from paper
“Infusion (GLP-1, 50 pmol/kg.h or saline) was started simultaneously with initiation of the test meals”
Assess appetite sensations with visual analogue scales
Use visual analogue scales to assess appetite sensations throughout the experiment
Note: Assessments continue throughout the entire experimental period
View evidence from paper
“Visual analogue scales were used to assess appetite sensations throughout the experiment and the palatability of the test meals”
Assess meal palatability
Use visual analogue scales to assess the palatability of the test meals
Note: Palatability assessment is conducted alongside appetite sensation assessment
View evidence from paper
“Visual analogue scales were used to assess appetite sensations throughout the experiment and the palatability of the test meals”
Provide ad libitum lunch
Provide an ad libitum lunch meal to measure spontaneous energy intake
Note: Lunch is self-selected to measure natural energy intake patterns
View evidence from paper
“spontaneous energy intake at the ad libitum lunch was reduced by 12% by GLP-1 infusion compared with saline”
Collect blood samples
Sample blood throughout the day for analysis of plasma hormone and substrate levels
Note: Multiple blood samples collected at various time points
View evidence from paper
“Blood was sampled throughout the day for analysis of plasma hormone and substrate levels”