Visual Analogue Scale Assessment
Objective: To assess appetite sensations and palatability of test meals using visual analogue scales throughout an experiment examining the effect of GLP-1 infusion on satiety and energy intake
Protocol Steps
Study design setup
Conduct a placebo-controlled, randomized, blinded, crossover study with 20 young, healthy, normal-weight men
Note: Study is randomized, blinded, and uses a crossover design
View evidence from paper
“20 young, healthy, normal-weight men participated in a placebo-controlled, randomized, blinded, crossover study”
Initiate infusion and test meal
Start intravenous infusion of either GLP-1 (50 pmol/kg.h) or saline simultaneously with initiation of the energy-fixed breakfast test meal
Note: Infusion and meal initiation must be simultaneous
View evidence from paper
“Infusion (GLP-1, 50 pmol/kg.h or saline) was started simultaneously with initiation of the test meals”
Assess appetite sensations with visual analogue scales
Use visual analogue scales to assess appetite sensations throughout the experiment
Note: Assessments continue throughout the entire experimental period
View evidence from paper
“Visual analogue scales were used to assess appetite sensations throughout the experiment and the palatability of the test meals”
Assess meal palatability
Use visual analogue scales to assess the palatability of the test meals
Note: Palatability assessment is conducted alongside appetite sensation assessment
View evidence from paper
“Visual analogue scales were used to assess appetite sensations throughout the experiment and the palatability of the test meals”
Provide ad libitum lunch
Provide an ad libitum lunch meal to measure spontaneous energy intake
Note: Lunch is self-selected to measure natural energy intake patterns
View evidence from paper
“spontaneous energy intake at the ad libitum lunch was reduced by 12% by GLP-1 infusion compared with saline”
Collect blood samples
Sample blood throughout the day for analysis of plasma hormone and substrate levels
Note: Multiple blood samples collected at various time points
View evidence from paper
“Blood was sampled throughout the day for analysis of plasma hormone and substrate levels”