Source Paper
NMDA receptor hypofunction produces concomitant firing rate potentiation and burst activity reduction in the prefrontal cortex
Mark E. Jackson, Houman Homayoun, Bita Moghaddam
Proceedings of the National Academy of Sciences • 2004
Working Memory Behavioral Testing
Objective: Assessment of working memory performance in rats treated with NMDA antagonists to correlate cognitive deficits with neural firing patterns in the prefrontal cortex
This is a Working Memory Behavioral Testing protocol using rat as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 1 materials. Extracted from a 2004 paper published in Proceedings of the National Academy of Sciences.
Model and subjects
rat • Not specified • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
NMDA antagonist administration • Ensemble neural recording • Working memory behavioral assessment
Primary readouts
- Working memory performance impairment
- Prefrontal cortex neuron firing rates
- Burst activity in prefrontal cortex neurons
- Single spike discharge patterns
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
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NMDA antagonist administration
Treat rats with NMDA antagonist at doses that impair working memory performance
Note: Doses selected based on ability to impair working memory
View evidence from paper
“NMDA antagonist treatment, at doses that impaired working memory, potentiated the firing rate”
Ensemble neural recording
Perform ensemble recording from prefrontal cortex neurons in freely moving rats during behavioral testing
Note: Recording conducted in freely moving animals to assess neural activity during working memory tasks
View evidence from paper
“Using ensemble recording in freely moving rats, we found that NMDA antagonist treatment”
Working memory behavioral assessment
Assess working memory performance in rats treated with NMDA antagonists
Note: Specific behavioral task details not provided in methods section
View evidence from paper
“NMDA antagonist treatment, at doses that impaired working memory, potentiated the firing rate”
Behavioral stereotypy observation
Monitor and record behavioral stereotypy expression during neural recording
Note: Stereotypy correlated with firing rate potentiation
View evidence from paper
“This potentiation, which correlated with expression of behavioral stereotypy, resulted from an increased number”