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Papers/Kuehner et al. 2023

Social defeat stress induces genome-wide 5mC and 5hmC alterations in the mouse brain

Kuehner JN, Walia NR, Seong R, et al. G3 (Bethesda, Md.) 2023;13(8):jkad114.

The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made

Tests in this paper

  • Social interaction test
  • Sucrose preference test

Social interaction test

Compare with 6 other papers
SettingValuePaper’s words
Session duration
  • 2.5 min

“two consecutive sessions of 2.5 min each”
Arena size
  • 40 × 40 cm height

“The arena was a white open field (40 × 40 cm height; Maze Engineers)”
Other settings in the paper
Red lamp lights

complete darkness supplemented with red lamp lights

“maintained in complete darkness supplemented with red lamp lights”
Consecutive sessions

two

“two consecutive sessions of 2.5 min each”
After the final defeat

24 h

“24 h after the final defeat, defeated and control mice were introduced into the open field”
Enclosure

10 × 6.5 × 30 cm high

“enclosure: 10 × 6.5 × 30 cm high”
Wire mesh

10 × 6.5 × 8 cm high

“wire mesh: 10 × 6.5 × 8 cm high”
Empty wire enclosure

No target

placed at one end of the arena in the center of the interaction zone

“During the first session (“no target”), the open arena contained an empty wire enclosure (enclosure: 10 × 6.5 × 30 cm high; wire mesh: 10 × 6.5 × 8 cm high; Maze Engineers) placed at one end of the arena in the center of the interaction zone”
Rounds of SI

LSDS cohorts

2

“Animals in the LSDS cohorts underwent 2 rounds of SI”
Second round

LSDS cohorts

4 weeks later

“Animals in the LSDS cohorts underwent 2 rounds of SI, one 24 h after the final defeat and a second round 4 weeks later”
Susceptible animals

SIR < 1

“Susceptible animals were defined as having a SIR < 1”
Resilient animals

SIR ≥1

“resilient animals had an SIR ≥1”
Independent scores

two

“two independent scores were used and averaged”
Behavior tests occurred during

the beginning of their dark cycle

“Social defeats and behavior tests occurred during the beginning of their dark cycle”
Apparatus
White open field

Maze Engineers

“The arena was a white open field (40 × 40 cm height; Maze Engineers)”
Wire enclosure

Maze Engineers

“an empty wire enclosure (enclosure: 10 × 6.5 × 30 cm high; wire mesh: 10 × 6.5 × 8 cm high; Maze Engineers)”
Red lamp lights
“maintained in complete darkness supplemented with red lamp lights”
Video recordings
“Video recordings were used to score interaction and avoidance behaviors”
Stopwatch
“All videos were scored manually using a stopwatch”

Sucrose preference test

Compare with 4 other papers
SettingValuePaper’s words
Bottles

2

“Mice were provided 2 bottles daily”
Fresh sucrose solution

1% (wt/vol)

“the other with 1% (wt/vol) fresh sucrose solution”
Position

counterbalanced

“that was counterbalanced for position”
Habituated

3 days

“Animals were habituated for 3 days”
Access to water and sucrose

Habituated

24-h

“habituated for 3 days with 24-h access to water and sucrose”
Preference testing

4 days

“Preference testing occurred for 4 days”
Access to water and sucrose

Preference testing

12 h

“Preference testing occurred for 4 days and the animals were given access to water and sucrose after the social defeat session for 12 h”
Bottles were weighed

the following morning

“Bottles were weighed the following morning”
Behavior tests occurred during

the beginning of their dark cycle

“Social defeats and behavior tests occurred during the beginning of their dark cycle”
Apparatus
Bottles
“Mice were provided 2 bottles daily”

Tests in this paper

  • Social interaction test
  • Sucrose preference test

The paper

Species: mice

“C57BL/6J male (Jackson Labs, stock #:000664) mice were used as experimental animals”
Read the paper (doi:10.1093/g3journal/jkad114)Full text on PubMed Central (PMC10411578)

Quotes are shown under the paper’s CC BY license.

How far this was checked

The test records were read once by AI and checked by code. No person has reviewed them yet.

How records are made