How 7 papers set up this test.
AI read these papers and code checked every quote. No person has reviewed the records yet. How records are made
Inner dimensions of the arena (floor and wall height)
| Paper | Value | Paper’s words |
|---|---|---|
| Dutta Gupta et al. 2026Rats |
| “featured three adjacent compartments (40 × 80 × 40 cm; L × W × H)” |
| Liu et al. 2024Mice |
| “Each social interaction chamber (30 × 30 × 30 cm) contained dividing walls with an open middle section to allow access.” |
| Kuehner et al. 2023Mice |
| “The arena was a white open field (40 × 40 cm height; Maze Engineers)” |
| Vanderplow et al. 2022Rats |
| “Social interaction was assessed in a gray rectangular box (80-cm length × 40.5-cm width × 40-cm height)” |
| Drapeau et al. 2018Mice |
| “were tested in a three-chambered apparatus (Nadler et al., 2004)” “in a regular clean cage during a 10-min test session as previously described (Scattoni et al., 2011)” |
Not in the Methods of Nguyen et al. 2025, Wen et al. 2024.
How long one session in the apparatus lasts
| Paper | Value | Paper’s words |
|---|---|---|
| Nguyen et al. 2025Mice |
| “2pSI was a 25-min session” |
| Liu et al. 2024Mice |
| “Mice were given free access to the apparatus for 10 min (in the absence of other mice) to habituate” “For each phase, the test mice explored the entire arena throughout the 10-min trial.” |
| Wen et al. 2024Mice |
| “During the habituation session, cylinders in the two side chambers were empty” “allowed to explore the arena freely for 10 min” “Then it is followed with 10 min testing session” |
| Kuehner et al. 2023Mice |
| “two consecutive sessions of 2.5 min each” |
| Vanderplow et al. 2022Rats |
| “Habituation session: Rats were placed in the middle chamber of an empty apparatus and allowed free exploration of the entire arena for 10 minutes” “Sociability session: Twenty-four hours following habituation” “Test rats were placed in the center chamber and allowed 7 minutes of exploration of the entire arena” “Social recognition session: Immediately following the sociability session” “Test rats were then allowed 7 minutes of free exploration” |
| Drapeau et al. 2018Mice |
| “The subject mouse was first placed in the central, neutral chamber and allowed to explore for 10 min with all doors closed” “freely explore the three empty chambers for an additional 10 min” “the subject was returned to the apparatus for a 10-min testing phase” “Male-female social interactions were evaluated in in a regular clean cage during a 10-min test session” |
| Dutta Gupta et al. 2026Rats |
| “Rats were habituated to the empty arena for 10 min” |
Settings outside the list above, named as each paper names them.
| Paper | Setting and value | Paper’s words |
|---|---|---|
| Wen et al. 2024Mice | Acclimated in the testing room at least 30 min | “All animals were acclimated in the testing room at least 30 min before testing starts” |
| Dutta Gupta et al. 2026Rats | Acclimated to a novel test room briefly | “Animals were briefly acclimated to a novel test room” |
| Nguyen et al. 2025Mice | Acclimated to the behavioral testing room at least 1 h | “mice were acclimated to the behavioral testing room for at least 1 h prior to being tested” |
| Dutta Gupta et al. 2026Rats | After the administration of either sham or TMS, Posttreatment 2 d | “Posttreatment social behavior was assessed using a different set of trapped conspecifics in the three-chamber test 2 d after the administration of either sham or TMS” |
| Kuehner et al. 2023Mice | After the final defeat 24 h | “24 h after the final defeat, defeated and control mice were introduced into the open field” |
| Dutta Gupta et al. 2026Rats | After the water maze 10–12 d | “The three-chamber social interaction test was conducted 10–12 d after the water maze” |
| Nguyen et al. 2025Mice | Apparatus was cleaned, Between test mice unscented disinfectant | “Between test mice, the apparatus was cleaned with an unscented disinfectant (Seventh Generation Inc., USA)” |
| Drapeau et al. 2018Mice | Before two tests, three-chambered apparatus at least 2 h | “with at least 2 h before two tests” |
| Kuehner et al. 2023Mice | Behavior tests occurred during the beginning of their dark cycle | “Social defeats and behavior tests occurred during the beginning of their dark cycle” |
| Drapeau et al. 2018Mice | Behavioral experiments were conducted between 9 A.M. and 5 P.M. | “Behavioral experiments were conducted between 9 A.M. and 5 P.M.” |
| Wen et al. 2024Mice | Chambers three | “a custom made matte black plastic box arena with three chambers and openings between the chambers” |
| Vanderplow et al. 2022Rats | Chambers 3 interconnecting chambers | “The box was divided into 3 interconnecting chambers” |
| Dutta Gupta et al. 2026Rats | Cleaned between test subjects 70% alcohol | “The arena and enclosures were cleaned between test subjects with 70% alcohol” |
| Liu et al. 2024Mice | Close interaction with the nose point within 2 cm of the enclosure 2 cm | “the time spent in close interaction with the nose point within 2 cm of the enclosure was also recorded” |
| Kuehner et al. 2023Mice | Consecutive sessions two | “two consecutive sessions of 2.5 min each” |
| Wen et al. 2024Mice | Cylinder, testing session a novel mouse was placed in the cylinder in one chamber and a novel object was placed in the cylinder in the other chamber | “Then it is followed with 10 min testing session, during which a novel mouse was placed in the cylinder in one chamber and a novel object was placed in the cylinder in the other chamber” |
| Vanderplow et al. 2022Rats | Cylinder wire cage 15-cm diameter and 30-cm height | “A cylinder wire cage (15-cm diameter and 30-cm height)” |
| Vanderplow et al. 2022Rats | Cylinder wire cage placed in the corners of each end chamber | “A cylinder wire cage (15-cm diameter and 30-cm height), which allows for animal auditory, visual, and olfactory information transfer, was placed in the corners of each end chamber” |
| Wen et al. 2024Mice | Cylinders, habituation session empty | “During the habituation session, cylinders in the two side chambers were empty” |
| Vanderplow et al. 2022Rats | Digital stopwatch certified resolution of 0.01 seconds and an accuracy of 0.0005% | “a digital stopwatch with a certified resolution of 0.01 seconds and an accuracy of 0.0005%” |
| Vanderplow et al. 2022Rats | Divider contains openings | “The divider contains openings that allow the animals to freely navigate between chambers.” |
| Nguyen et al. 2025Mice | Each conspecific mouse was used up to three times per test day | “Each conspecific mouse was used up to three times per test day” |
| Nguyen et al. 2025Mice | Empty end zone an identical area | “In the non-compartment end of the maze, an identical area was demarcated as the empty end zone” |
| Drapeau et al. 2018Mice | Empty wire cages, three-chambered apparatus two | “two empty wire cages” |
| Kuehner et al. 2023Mice | Empty wire enclosure, no target placed at one end of the arena in the center of the interaction zone | “During the first session (“no target”), the open arena contained an empty wire enclosure (enclosure: 10 × 6.5 × 30 cm high; wire mesh: 10 × 6.5 × 8 cm high; Maze Engineers) placed at one end of the arena in the center of the interaction zone” |
| Kuehner et al. 2023Mice | Enclosure 10 × 6.5 × 30 cm high | “enclosure: 10 × 6.5 × 30 cm high” |
| Nguyen et al. 2025Mice | End-compartments 5 × 7 cm | “two of which included end-compartments (5 × 7 cm)” |
| Dutta Gupta et al. 2026Rats | Exclusion criteria failure to sample all available choices | “Exclusion criteria were failure on the nonspatial, cued variant of the water maze, failure to sample all available choices in the social and olfactory tests” |
| Vanderplow et al. 2022Rats | Experimenter blind to conditions | “This test was carried out by, and analyzed by, an experimenter blind to conditions.” |
| Drapeau et al. 2018Mice | Females, Male-female social interactions 20 | “A total of 20 females were used for this test” |
| Vanderplow et al. 2022Rats | Following habituation, Sociability session Twenty-four hours | “Sociability session: Twenty-four hours following habituation” |
| Dutta Gupta et al. 2026Rats | Following the baseline social cognition test, Experiment 2 Three to five days | “Three to five days following the baseline social cognition test, young and aged rats in Experiment 2 randomly received TMS or sham stimulation” |
| Vanderplow et al. 2022Rats | Following the sociability session, Social recognition session Immediately | “Social recognition session: Immediately following the sociability session” |
| Nguyen et al. 2025Mice | Habituated to the maze 5 min | “test mice were first habituated to the maze for 5 min (with no stimuli inside the compartments)” |
| Nguyen et al. 2025Mice | Handled by experimenters 2 min/ day for 3 consecutive days | “mice were handled by experimenters for 2 min/ day for 3 consecutive days prior to being tested behaviorally” |
| Kuehner et al. 2023Mice | Independent scores two | “two independent scores were used and averaged” |
| Nguyen et al. 2025Mice | Interaction the time that the center-point of the test animal was tracked inside defined zones adjacent to the stimulus compartments | “Interaction was operationally defined as the time that the center-point of the test animal was tracked inside defined zones adjacent to the stimulus compartments” |
| Dutta Gupta et al. 2026Rats | Investigation of either the social or nonsocial stimulus within ∼3 cm of an enclosure with its nose oriented toward the front opening | “Investigation of either the social or nonsocial stimulus was considered when the experimental animal was within ∼3 cm of an enclosure with its nose oriented toward the front opening” |
| Nguyen et al. 2025Mice | Left or right compartment, Phase 1 counterbalanced | “During Phase 1, the test mouse could interact (through the barrier panels) with a same-sex novel conspecific or a novel object (left or right compartment, counterbalanced)” |
| Drapeau et al. 2018Mice | Low light, Male-female social interactions 10 lux | “under low light (10 lux) conditions” |
| Drapeau et al. 2018Mice | Mice were brought to the front room of the testing area at least half an hour | “Mice were brought to the front room of the testing area at least half an hour before the start of experiments” |
| Dutta Gupta et al. 2026Rats | Openings 20 × 18 cm | “divided by walls with 20 × 18 cm openings” |
| Wen et al. 2024Mice | Order of tests Day 8 | “Day 8, three-chamber sociability task” |
| Vanderplow et al. 2022Rats | Partition transparent | “using a transparent partition such that the 2 end chambers were larger than the central middle chamber” |
| Wen et al. 2024Mice | Placed in the middle chamber | “An animal was placed in the middle chamber” |
| Vanderplow et al. 2022Rats | Placed in, Habituation session the middle chamber | “Habituation session: Rats were placed in the middle chamber” |
| Vanderplow et al. 2022Rats | Placed in, Sociability session the center chamber | “Test rats were placed in the center chamber” |
| Wen et al. 2024Mice | Plastic cylinder with holes | “The two side chambers contain a plastic cylinder with holes” |
| Drapeau et al. 2018Mice | Positioned, Male-female social interactions 10 cm above the cage | “positioned 10 cm above the cage” |
| Kuehner et al. 2023Mice | Red lamp lights complete darkness supplemented with red lamp lights | “maintained in complete darkness supplemented with red lamp lights” |
| Vanderplow et al. 2022Rats | Removed from analysis did not enter both end chambers during the testing phases | “Any rats that did not enter both end chambers during the testing phases were removed from analysis” |
| Kuehner et al. 2023Mice | Resilient animals SIR ≥1 | “resilient animals had an SIR ≥1” |
| Wen et al. 2024Mice | Rest in between tests at least a 1-day | “Animals were given at least a 1-day rest in between tests” |
| Drapeau et al. 2018Mice | Reuse the same female, Male-female social interactions more than twice on the same day | “avoid to reuse the same female more than twice on the same day” |
| Kuehner et al. 2023Mice | Rounds of SI, LSDS cohorts 2 | “Animals in the LSDS cohorts underwent 2 rounds of SI” |
| Nguyen et al. 2025Mice | Runways 30 × 7 cm | “a T-shaped, opaque acrylic maze consisting of three runways (30 × 7 cm)” |
| Nguyen et al. 2025Mice | Runways three | “consisting of three runways” |
| Drapeau et al. 2018Mice | Sampling rate, Male-female social interactions 250-kHz | “an ultrasonic microphone with a 250-kHz sampling rate” |
| Dutta Gupta et al. 2026Rats | Scoring validated by an experimenter blind to group identity | “Scoring was validated by an experimenter blind to group identity” |
| Kuehner et al. 2023Mice | Second round, LSDS cohorts 4 weeks later | “Animals in the LSDS cohorts underwent 2 rounds of SI, one 24 h after the final defeat and a second round 4 weeks later” |
| Vanderplow et al. 2022Rats | Sessions 3 sessions | “Testing occurred in 3 sessions” |
| Dutta Gupta et al. 2026Rats | Small enclosure 38 × 20 × 20 cm | “an identical small enclosure (38 × 20 × 20 cm; L × W × H)” |
| Nguyen et al. 2025Mice | Social behavior phases 10-min | “two consecutive 10-min social behavior phases” |
| Nguyen et al. 2025Mice | Social behavior phases two | “two consecutive 10-min social behavior phases” |
| Dutta Gupta et al. 2026Rats | Social novelty trial followed immediately | “In the social novelty trial that followed immediately” |
| Liu et al. 2024Mice | Start position, sociability the middle chamber of the apparatus | “mice were placed into the middle chamber of the apparatus with one outer chamber containing one mouse ('stranger 1') confined in a wire cup and the other chamber containing a Lego block” |
| Kuehner et al. 2023Mice | Susceptible animals SIR < 1 | “Susceptible animals were defined as having a SIR < 1” |
| Nguyen et al. 2025Mice | Test mouse could interact (through the barrier panels) with, Phase 1 a same-sex novel conspecific or a novel object | “During Phase 1, the test mouse could interact (through the barrier panels) with a same-sex novel conspecific or a novel object (left or right compartment, counterbalanced)” |
| Vanderplow et al. 2022Rats | The order from first to last Y-maze, open field, novel object recognition, social interaction, and social novelty | “with the order from first to last as the following: Y-maze, open field, novel object recognition, social interaction, and social novelty” |
| Nguyen et al. 2025Mice | The test mouse could interact with, Phase 2 the original, “familiar” conspecific, or the new “unfamiliar” conspecific | “During Phase 2, the novel object was replaced with another novel same-sex conspecific; the test mouse could interact with the original, “familiar” conspecific, or the new “unfamiliar” conspecific” |
| Drapeau et al. 2018Mice | Unfamiliar mice were not used more than, three-chambered apparatus twice a day | “Unfamiliar mice were not used more than twice a day” |
| Vanderplow et al. 2022Rats | Video recordings one-third of real-time speed | “video recordings were slowed down to one-third of real-time speed” |
| Kuehner et al. 2023Mice | Wire mesh 10 × 6.5 × 8 cm high | “wire mesh: 10 × 6.5 × 8 cm high” |
| Dutta Gupta et al. 2026Rats | Zones of interest defined around each stimulus cage | “with zones of interest defined around each stimulus cage” |
What each paper names for this test. Vendors and models appear as the paper prints them.
| Paper | Apparatus | Paper’s words |
|---|---|---|
| Dutta Gupta et al. 2026 | Three-chamber apparatus Maze Engineers | “The custom apparatus (Maze Engineers) featured three adjacent compartments” |
| Dutta Gupta et al. 2026 | ANY-maze software Stoelting | “recorded using the ANY-maze software (Stoelting)” |
| Dutta Gupta et al. 2026 | Small enclosure | “an identical small enclosure (38 × 20 × 20 cm; L × W × H)” |
| Nguyen et al. 2025 | EthoVision XT Noldus | “Behavior was scored using an automated tracking system, EthoVision XT version 14 (Noldus, Leesburg, VA)” |
| Nguyen et al. 2025 | T-shaped, opaque acrylic maze TAP Plastics | “a T-shaped, opaque acrylic maze consisting of three runways (30 × 7 cm), two of which included end-compartments (5 × 7 cm) that held a novel conspecific or object (TAP Plastics, San Jose, CA)” |
| Nguyen et al. 2025 | Transparent, air-holed acrylic barrier panels | “The compartment enclosures were created by the insertion of transparent, air-holed acrylic barrier panels” |
| Nguyen et al. 2025 | Novel object | “a same-sex novel conspecific or a novel object” |
| Nguyen et al. 2025 | Unscented disinfectant Seventh Generation Inc. | “the apparatus was cleaned with an unscented disinfectant (Seventh Generation Inc., USA)” |
| Liu et al. 2024 | Social interaction chamber | “Each social interaction chamber (30 × 30 × 30 cm) contained dividing walls with an open middle section to allow access.” |
| Liu et al. 2024 | Wire cups | “Both outer chambers contained wire cups.” |
| Liu et al. 2024 | EthoVision XT 14 Noldus Information Technology | “the time spent in close interaction with the nose point within 2 cm of the enclosure was also recorded (EthoVision XT 14, Noldus Information Technology)” |
| Wen et al. 2024 | EthoVision XT 17 system Noldus Information Technology | “EthoVision XT 17 system (Noldus Information Technology, Wageningen, Netherlands)” |
| Wen et al. 2024 | Custom made matte black plastic box arena | “a custom made matte black plastic box arena with three chambers and openings between the chambers” |
| Wen et al. 2024 | Plastic cylinder | “The two side chambers contain a plastic cylinder with holes” |
| Kuehner et al. 2023 | White open field Maze Engineers | “The arena was a white open field (40 × 40 cm height; Maze Engineers)” |
| Kuehner et al. 2023 | Wire enclosure Maze Engineers | “an empty wire enclosure (enclosure: 10 × 6.5 × 30 cm high; wire mesh: 10 × 6.5 × 8 cm high; Maze Engineers)” |
| Kuehner et al. 2023 | Red lamp lights | “maintained in complete darkness supplemented with red lamp lights” |
| Kuehner et al. 2023 | Video recordings | “Video recordings were used to score interaction and avoidance behaviors” |
| Kuehner et al. 2023 | Stopwatch | “All videos were scored manually using a stopwatch” |
| Vanderplow et al. 2022 | Overhead digital video camera | “All animal behaviors were videotaped with an overhead digital video camera.” |
| Vanderplow et al. 2022 | Gray rectangular box Maze Engineers | “Social interaction was assessed in a gray rectangular box (80-cm length × 40.5-cm width × 40-cm height) purchased from Maze Engineers” |
| Vanderplow et al. 2022 | Cylinder wire cage | “A cylinder wire cage (15-cm diameter and 30-cm height)” |
| Vanderplow et al. 2022 | Digital stopwatch | “quantified using a digital stopwatch with a certified resolution of 0.01 seconds and 0.0005% accuracy” |
| Drapeau et al. 2018 | Noldus Ethovision | “Animal activity was recorded by video tracking (Noldus Ethovision)” |
| Drapeau et al. 2018 | Regular clean cage | “a regular clean cage (28 cm long × 18 cm wide × 12 cm high)” |
| Drapeau et al. 2018 | Three-chambered apparatus | “were tested in a three-chambered apparatus (Nadler et al., 2004)” |
| Drapeau et al. 2018 | Wire cages | “two empty wire cages” |
| Drapeau et al. 2018 | Inanimate object (black cone) | “containing either an inanimate object (black cone)” |
| Drapeau et al. 2018 | Ultrasonic microphone Noldus, Ultravox XT | “an ultrasonic microphone with a 250-kHz sampling rate (Noldus Ultravox XT)” |
| Drapeau et al. 2018 | Ultravox XT software | “spectrograms were displayed using the Ultravox XT software” |