Drapeau E, Riad M, Kajiwara Y, et al. eNeuro 2018;5(3):ENEURO.0046-18.2018.
The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made
| Setting | Value | Paper’s words |
|---|---|---|
| Beam size |
| “an elevated horizontal wood beam (100 cm long, 1 m above bedding)” “three different beams: 1 inch, ½ inch and ¼ inch diameter” |
| Beam height |
| “an elevated horizontal wood beam (100 cm long, 1 m above bedding)” |
| Number of trials |
| “scored on four consecutive trials per beam” |
| Other settings in the paper | ||
| Different beams | three | “three different beams” |
| Beam | wood | “an elevated horizontal wood beam” |
| Rest between trials on the same beam | 1 min | “with 1 min of rest between trials on the same beam” |
| Between each beam | 20–30 min | “20–30 min between each beam” |
| Did not reach the box after | 2 min | “Mice that did not reach the box after 2 min” |
| Allowed to stay inside | 1 min | “allowed to stay inside for 1 min” |
| Bright light | Near one end bright light | “Subjects were placed near one end in bright light” |
| Darkness | Far end with the dark box darkness | “the far end with the dark box was placed in darkness” |
| Mice were brought to the front room of the testing area | at least half an hour | “Mice were brought to the front room of the testing area at least half an hour before the start of experiments” |
| Behavioral experiments were conducted | between 9 A.M. and 5 P.M. | “Behavioral experiments were conducted between 9 A.M. and 5 P.M.” |
| Apparatus | ||
| Wood beam | “an elevated horizontal wood beam (100 cm long, 1 m above bedding)” | |
| Dark box | “to a safe dark box” | |
| Setting | Value | Paper’s words |
|---|---|---|
| Walkway size |
| “an elevated runway (dimensions: 152 cm long × 10 cm wide)” |
| Number of runs |
| “to record paw prints on two consecutive runs” |
| Not in the Methods | Run duration criterion | |
| Other settings in the paper | ||
| Training runs | three | “After three training runs” |
| Analysis of | 50 cm | “for analysis of 50 cm” |
| The record displaying | the clearest prints and most consistent gait | “The record displaying the clearest prints and most consistent gait” |
| Nontoxic paint | different colors for hind and front paws | “coated in nontoxic paint (different colors for hind and front paws)” |
| Lined with | white paper | “lined with white paper” |
| Mice were brought to the front room of the testing area | at least half an hour | “Mice were brought to the front room of the testing area at least half an hour before the start of experiments” |
| Behavioral experiments were conducted | between 9 A.M. and 5 P.M. | “Behavioral experiments were conducted between 9 A.M. and 5 P.M.” |
| Apparatus | ||
| Elevated runway | “an elevated runway (dimensions: 152 cm long × 10 cm wide)” | |
| White paper | “lined with white paper” | |
| Nontoxic paint | “paws were coated in nontoxic paint” | |
| Setting | Value | Paper’s words |
|---|---|---|
| Number of trials |
| “Test was repeated three times with a 3-min interval between trials” |
| Other settings in the paper | ||
| Interval between trials | 3-min | “Test was repeated three times with a 3-min interval between trials” |
| Attempts | Each trial five | “Each trial consisted in five attempts in quick successions” |
| Attempts in quick successions | the best value was recorded | “five attempts in quick successions for which the best value was recorded” |
| Peak tension | the force at the moment when the mouse lost its grip | “the force at the moment when the mouse lost its grip was recorded as the peak tension” |
| Muscle strength | Forelimb | “Forelimb muscle strength and function was evaluated” |
| Mice were brought to the front room of the testing area | at least half an hour | “Mice were brought to the front room of the testing area at least half an hour before the start of experiments” |
| Behavioral experiments were conducted | between 9 A.M. and 5 P.M. | “Behavioral experiments were conducted between 9 A.M. and 5 P.M.” |
| Apparatus | ||
| Strength meter | Ametek | “was evaluated with a strength meter (Ametek)” |
| Pull bar connected to a tension meter | “while grasping a pull bar connected to a tension meter” | |
| Setting | Value | Paper’s words |
|---|---|---|
| Session duration |
| “allowed to explore and bury the marbles during a 15-min session” “The subject was first placed in the empty cage for a 5-min habituation” |
| Other settings in the paper | ||
| Regular clean cage | 28 cm long × 18 cm wide × 12 cm high | “a regular clean cage (28 cm long × 18 cm wide × 12 cm high)” |
| Fresh bedding | 3 cm | “with 3 cm of fresh bedding” |
| Dark blue glass marbles | 20 | “20 dark blue glass marbles (15 mm in diameter)” |
| Marbles | 15 mm in diameter | “20 dark blue glass marbles (15 mm in diameter)” |
| Arrangement | 4 × 5 | “positioned over the bedding equidistant in a 4 × 5 arrangement” |
| Marbles buried | >50% | “the number of marbles buried (>50% marble covered by bedding material)” |
| Scored for | Escape behavior 10 min | “Each test was scored for 10 min” |
| Mice were brought to the front room of the testing area | at least half an hour | “Mice were brought to the front room of the testing area at least half an hour before the start of experiments” |
| Behavioral experiments were conducted | between 9 A.M. and 5 P.M. | “Behavioral experiments were conducted between 9 A.M. and 5 P.M.” |
| Apparatus | ||
| Regular clean cage | “a regular clean cage (28 cm long × 18 cm wide × 12 cm high)” | |
| Dark blue glass marbles | “20 dark blue glass marbles (15 mm in diameter)” | |
| Fresh bedding | “with 3 cm of fresh bedding” | |
| Setting | Value | Paper’s words |
|---|---|---|
| Arena size |
| “Mice were tested in an open field (45 × 45 cm)” |
| Session duration |
| “Each mouse was allowed to explore the apparatus for 60 min” |
| Not in the Methods | Illumination | |
| Other settings in the paper | ||
| Measures were recorded | 10-min intervals | “Measures were recorded in 10-min intervals” |
| Virtually divided into | central and peripheral regions | “virtually divided into central and peripheral regions” |
| Spontaneous grooming bouts | 10 first minutes | “spontaneous grooming bouts observed during the 10 first minutes of the open field test” |
| Wall rears | while touching a side of the open field | “wall rears (while touching a side of the open field)” |
| Mice were brought to the front room of the testing area | at least half an hour | “Mice were brought to the front room of the testing area at least half an hour before the start of experiments” |
| Behavioral experiments were conducted | between 9 A.M. and 5 P.M. | “Behavioral experiments were conducted between 9 A.M. and 5 P.M.” |
| Apparatus | ||
| Noldus Ethovision | “Animal activity was recorded by video tracking (Noldus Ethovision)” | |
| Open field | “Mice were tested in an open field (45 × 45 cm)” | |
| Setting | Value | Paper’s words |
|---|---|---|
| Rod speed |
| “a linear acceleration from 4 to 40 rpm” |
| Trial duration |
| “Each trial lasted for a maximum of 5 min” |
| Number of trials |
| “for three trials per day on two consecutive days” |
| Other settings in the paper | ||
| Accelerating rod | 3 cm in diameter | “an elevated accelerating rod (3 cm in diameter)” |
| Consecutive days | two consecutive days | “for three trials per day on two consecutive days” |
| Interval | Between trials 20-min | “A 20-min interval was used between trials” |
| Acceleration | linear | “a linear acceleration from 4 to 40 rpm” |
| Mice were brought to the front room of the testing area | at least half an hour | “Mice were brought to the front room of the testing area at least half an hour before the start of experiments” |
| Behavioral experiments were conducted | between 9 A.M. and 5 P.M. | “Behavioral experiments were conducted between 9 A.M. and 5 P.M.” |
| Apparatus | ||
| Rotarod | Omnitech Electronics Inc | “was assessed in the Rotarod test (Omnitech Electronics Inc)” |
| Setting | Value | Paper’s words |
|---|---|---|
| Session duration |
| “The subject mouse was first placed in the central, neutral chamber and allowed to explore for 10 min with all doors closed” “freely explore the three empty chambers for an additional 10 min” “the subject was returned to the apparatus for a 10-min testing phase” “Male-female social interactions were evaluated in in a regular clean cage during a 10-min test session” |
| Arena size |
| “were tested in a three-chambered apparatus (Nadler et al., 2004)” “in a regular clean cage during a 10-min test session as previously described (Scattoni et al., 2011)” |
| Other settings in the paper | ||
| Empty wire cages | Three-chambered apparatus two | “two empty wire cages” |
| Unfamiliar mice were not used more than | Three-chambered apparatus twice a day | “Unfamiliar mice were not used more than twice a day” |
| Before two tests | Three-chambered apparatus at least 2 h | “with at least 2 h before two tests” |
| Low light | Male-female social interactions 10 lux | “under low light (10 lux) conditions” |
| Females | Male-female social interactions 20 | “A total of 20 females were used for this test” |
| Reuse the same female | Male-female social interactions more than twice on the same day | “avoid to reuse the same female more than twice on the same day” |
| Sampling rate | Male-female social interactions 250-kHz | “an ultrasonic microphone with a 250-kHz sampling rate” |
| Positioned | Male-female social interactions 10 cm above the cage | “positioned 10 cm above the cage” |
| Mice were brought to the front room of the testing area | at least half an hour | “Mice were brought to the front room of the testing area at least half an hour before the start of experiments” |
| Behavioral experiments were conducted | between 9 A.M. and 5 P.M. | “Behavioral experiments were conducted between 9 A.M. and 5 P.M.” |
| Apparatus | ||
| Noldus Ethovision | “Animal activity was recorded by video tracking (Noldus Ethovision)” | |
| Regular clean cage | “a regular clean cage (28 cm long × 18 cm wide × 12 cm high)” | |
| Three-chambered apparatus | “were tested in a three-chambered apparatus (Nadler et al., 2004)” | |
| Wire cages | “two empty wire cages” | |
| Inanimate object (black cone) | “containing either an inanimate object (black cone)” | |
| Ultrasonic microphone | Noldus, Ultravox XT | “an ultrasonic microphone with a 250-kHz sampling rate (Noldus Ultravox XT)” |
| Ultravox XT software | “spectrograms were displayed using the Ultravox XT software” | |
| Methods cited to other work | ||
| Three-chambered apparatus | Nadler et al., 2004 | “a three-chambered apparatus (Nadler et al., 2004)” |
| Male-female social interaction session | Scattoni et al., 2011 | “as previously described (Scattoni et al., 2011)” |
| Setting | Value | Paper’s words |
|---|---|---|
| Session duration |
| “given 15 min to freely explore the three arms of the maze” |
| Arm size |
| “three 5-cm-wide and 50-cm-long arms” |
| Other settings in the paper | ||
| Arms | three | “three 5-cm-wide and 50-cm-long arms” |
| Each set | 130° | “each set 130° from each other” |
| An entry occurs when | all four limbs are within the arm | “An entry occurs when all four limbs are within the arm” |
| A successful score | three successive choices that includes one instance of each arm | “A successful score is defined by three successive choices that includes one instance of each arm” |
| A type 1 error | three consecutive choices where the first and third choices are identical | “A type 1 error is determined by three consecutive choices where the first and third choices are identical” |
| A type 2 error | three consecutive choices where the second and third choices are identical | “A type 2 error is defined by three consecutive choices where the second and third choices are identical” |
| Perseverance | three or more repetitive entries in the same arm | “Perseverance is defined as three or more repetitive entries in the same arm” |
| Mice were brought to the front room of the testing area | at least half an hour | “Mice were brought to the front room of the testing area at least half an hour before the start of experiments” |
| Behavioral experiments were conducted | between 9 A.M. and 5 P.M. | “Behavioral experiments were conducted between 9 A.M. and 5 P.M.” |
| Apparatus | ||
| Y-maze | “Mice were placed in the center of a Y-maze” | |