How 10 papers set up this test.
AI read these papers and code checked every quote. No person has reviewed the records yet. How records are made
Rotation speed of the rotarod rod: one fixed speed, or the start and end speeds of an accelerating run
| Paper | Value | Paper’s words |
|---|---|---|
| Johnston et al. 2025Mice |
| “rotation starting at 4 revolutions/min” “until a maximum speed of 40 revolutions/min” |
| Wen et al. 2024Mice |
| “For the training trial, mice were placed on the rotarod with a constant speed of four revolutions per minute (rpm) for 5 min” “For the testing trial, the rotarod was set to accelerate from 4 to 40 rpm over the course of 5 min” |
| Trabjerg et al. 2023Mice |
| “The speed was increased from 5 to 40 RPM within 140 s for the C57Bl/6J rotenone etomoxir experiment” “The speed was optimized for the other experiments” “Starting at 5 RPM and ending at 40 RPM at 140 s” |
| Somebang et al. 2021Mice |
| “a rotating rod that accelerated to 40 rpm over 300 s” |
| Trabjerg et al. 2021Mice |
| “an acceleration from 4 to 40 RPM over 5 min” |
| Drapeau et al. 2018Mice |
| “a linear acceleration from 4 to 40 rpm” |
| Xin et al. 2017Mice |
| “three 5-min training trials with the speed set to 10, 12 and 14 r.p.m.” “During testing, the rotarod speed was increased from 4 to 40 r.p.m. over a 5-min period” |
| Florez-Paz et al. 2016Mice |
| “The initial speed of rotation was 5 rpm and it accelerated to 20 rpm” |
Not in the Methods of Ahire et al. 2023, Rattner et al. 2020.
The fixed or longest length of one trial
| Paper | Value | Paper’s words |
|---|---|---|
| Johnston et al. 2025Mice |
| “Testing consisted of 5 min maximum latency” |
| Trabjerg et al. 2023Mice |
| “The cut-off time was set to 180 s” |
| Drapeau et al. 2018Mice |
| “Each trial lasted for a maximum of 5 min” |
| Wen et al. 2024Mice |
| “For the training trial, mice were placed on the rotarod with a constant speed of four revolutions per minute (rpm) for 5 min” “For the testing trial, the rotarod was set to accelerate from 4 to 40 rpm over the course of 5 min” |
| Xin et al. 2017Mice |
| “three 5-min training trials” “the rotarod speed was increased from 4 to 40 r.p.m. over a 5-min period” |
| Trabjerg et al. 2021Mice |
| “an acceleration from 4 to 40 RPM over 5 min” |
Not in the Methods of Ahire et al. 2023, Somebang et al. 2021, Rattner et al. 2020, Florez-Paz et al. 2016.
How many trials an animal gets in one session or day
| Paper | Value | Paper’s words |
|---|---|---|
| Johnston et al. 2025Mice |
| “On testing days, mice performed 3 trials of rotarod” |
| Wen et al. 2024Mice |
| “Each mouse was tested for three testing trials with 10–15 min to rest in between trials” |
| Trabjerg et al. 2023Mice |
| “Each animal received three trials per session” “Prior to the rotarod performance test, mice were trained on three different dates with three trials per session” |
| Somebang et al. 2021Mice |
| “Animals were assessed five trials/day for two days.” |
| Trabjerg et al. 2021Mice |
| “Each mouse was tested three times per test session” |
| Drapeau et al. 2018Mice |
| “for three trials per day on two consecutive days” |
| Xin et al. 2017Mice |
| “with three 5-min training trials with the speed set to 10, 12 and 14 r.p.m.” “trained on the rotarod for three consecutive days” |
| Florez-Paz et al. 2016Mice |
| “all the tests, each of which was repeated at least three times in each animal” |
Not in the Methods of Ahire et al. 2023, Rattner et al. 2020.
Settings outside the list above, named as each paper names them.
| Paper | Setting and value | Paper’s words |
|---|---|---|
| Wen et al. 2024Mice | Accelerate from 4 to 40 rpm over the course of, testing trial 5 min | “For the testing trial, the rotarod was set to accelerate from 4 to 40 rpm over the course of 5 min” |
| Somebang et al. 2021Mice | Accelerated to 40 rpm over 300 s | “a rotating rod that accelerated to 40 rpm over 300 s” |
| Drapeau et al. 2018Mice | Accelerating rod 3 cm in diameter | “an elevated accelerating rod (3 cm in diameter)” |
| Trabjerg et al. 2023Mice | Acceleration, the other experiments 2.5 RPM per 10 s | “The speed was optimized for the other experiments, using an acceleration of 2.5 RPM per 10 s” |
| Trabjerg et al. 2021Mice | Acceleration over 5 min | “an acceleration from 4 to 40 RPM over 5 min” |
| Drapeau et al. 2018Mice | Acceleration linear | “a linear acceleration from 4 to 40 rpm” |
| Trabjerg et al. 2021Mice | Acclimatized to the rotarod, before the first test session three consecutive days | “Mice were acclimatized to the rotarod over three consecutive days before the first test session” |
| Somebang et al. 2021Mice | After TBI three weeks | “three weeks after TBI” |
| Trabjerg et al. 2023Mice | All tests were performed between 10:00 and 16:00 in the lights-on cycle | “All tests were performed between 10:00 and 16:00 in the lights-on cycle” |
| Somebang et al. 2021Mice | Animals were assessed two days | “Animals were assessed five trials/day for two days.” |
| Drapeau et al. 2018Mice | Behavioral experiments were conducted between 9 A.M. and 5 P.M. | “Behavioral experiments were conducted between 9 A.M. and 5 P.M.” |
| Johnston et al. 2025Mice | Break, between trials >10 min | “a break of>10 min between trials” |
| Trabjerg et al. 2021Mice | Cleaned with 70% ethanol between each animal | “Test equipment was cleaned with 70% ethanol between each animal” |
| Drapeau et al. 2018Mice | Consecutive days two consecutive days | “for three trials per day on two consecutive days” |
| Trabjerg et al. 2023Mice | Data were excluded if the mice continuously jumped off the rotarod or turned in the wrong direction before acceleration | “Data were excluded if the mice continuously jumped off the rotarod or turned in the wrong direction before acceleration” |
| Florez-Paz et al. 2016Mice | Diameter rotating cylinder 5 cm | “the mice were placed on a 5 cm diameter rotating cylinder” |
| Trabjerg et al. 2023Mice | Ending at 40 RPM, the other experiments at 140 s | “Starting at 5 RPM and ending at 40 RPM at 140 s” |
| Trabjerg et al. 2021Mice | Evaluated weekly | “Mice were evaluated weekly by neurological score, grip strength, hangwire test, rotarod test” |
| Trabjerg et al. 2023Mice | Experimenters blinded to treatment groups | “The experimenters were blinded to treatment groups for all behavioral studies” |
| Rattner et al. 2020Mice | Experimenters blind to the genotype | “During all behavioral testing, the experimenters were blind to the genotype” |
| Trabjerg et al. 2023Mice | Exposed to behavioral tests in a randomized order | “The mice were exposed to behavioral tests in a randomized order” |
| Johnston et al. 2025Mice | Fitness testing once per month | “All participants underwent fitness testing once per month over a period of 6 months” |
| Trabjerg et al. 2023Mice | Full cleaning protocol, Between every test 70% ethanol | “Between every test, a full cleaning protocol by 70% ethanol was applied to the surfaces of the equipment” |
| Johnston et al. 2025Mice | Grip strength and rotarod tests were performed on the same day | “Grip strength and rotarod tests were performed on the same day” |
| Johnston et al. 2025Mice | Increasing steadily 7.2 revolutions/min^2 | “increasing steadily at 7.2 revolutions/min^2” |
| Drapeau et al. 2018Mice | Interval, between trials 20-min | “A 20-min interval was used between trials” |
| Ahire et al. 2023Mice | Lane four‐lane | “automated four‐lane rotarod tool” |
| Johnston et al. 2025Mice | Latency automatically recorded via the motion sensor on the apparatus | “their latency was automatically recorded via the motion sensor on the apparatus” |
| Trabjerg et al. 2023Mice | Mice were acclimatized to the laboratory 30 min before tests began | “mice were acclimatized to the laboratory 30 min before tests began” |
| Drapeau et al. 2018Mice | Mice were brought to the front room of the testing area at least half an hour | “Mice were brought to the front room of the testing area at least half an hour before the start of experiments” |
| Trabjerg et al. 2023Mice | Mice were evaluated, Park2 etomoxir experiment At day 22 | “At day 22, mice were evaluated for pathological motor and non-motor behavior using rotarod, grip strength, Y-maze, novel object recognition, and dark-light box test” |
| Trabjerg et al. 2023Mice | Mice were evaluated, Park2 rotenone washout etomoxir experiment At day 54 | “At day 54, mice were evaluated for pathological motor and non-motor behavior using rotarod, grip strength, Y-maze, and novel object recognition” |
| Trabjerg et al. 2023Mice | Mice were trained on, Prior to the rotarod performance test three different dates | “Prior to the rotarod performance test, mice were trained on three different dates with three trials per session” |
| Trabjerg et al. 2023Mice | Motor test, chronic rotenone mouse models day 60 | “motor test at day 60 (rotarod)” |
| Wen et al. 2024Mice | Order of tests Day 10 | “Day 10, rotarod” |
| Trabjerg et al. 2021Mice | Performed between 9 am and 2 pm | “All clinical behavioral tests were performed between 9 am and 2 pm” |
| Johnston et al. 2025Mice | Period 6 months | “All participants underwent fitness testing once per month over a period of 6 months” |
| Xin et al. 2017Mice | Period over which the rotarod speed was increased, testing 5-min | “the rotarod speed was increased from 4 to 40 r.p.m. over a 5-min period” |
| Trabjerg et al. 2021Mice | Placed in the room one hour before test sessions | “mice were placed in the room one hour before test sessions to allow time for acclimatization” |
| Wen et al. 2024Mice | Rest in between tests at least a 1-day | “Animals were given at least a 1-day rest in between tests” |
| Wen et al. 2024Mice | Rest in between trials, testing trials 10–15 min | “Each mouse was tested for three testing trials with 10–15 min to rest in between trials” |
| Johnston et al. 2025Mice | Rotating dowels, in half the lanes 50% smaller | “50% smaller rotating dowels in half the lanes” |
| Trabjerg et al. 2023Mice | Speed was increased, C57Bl/6J rotenone etomoxir experiment within 140 s | “The speed was increased from 5 to 40 RPM within 140 s for the C57Bl/6J rotenone etomoxir experiment” |
| Xin et al. 2017Mice | The tests were repeated once a week | “Thereafter, the tests were repeated once a week until the mice could no longer complete the tests.” |
| Xin et al. 2017Mice | Trained on the rotarod, training three consecutive days | “trained on the rotarod for three consecutive days” |
| Trabjerg et al. 2023Mice | Were defined by the presence of, At day 32 a minimum two of the following factors | “At day 32, animals, who did not show signs of disease, were defined by the presence of a minimum two of the following factors: lower grip strength, lower latency to fall on the rotarod, longer time to transverse the beam, pole or lower number of rears in the cylinder test” |
| Xin et al. 2017Mice | Were tested on the fourth day | “The mice were trained for three consecutive days and were tested on the fourth day.” |
What each paper names for this test. Vendors and models appear as the paper prints them.
| Paper | Apparatus | Paper’s words |
|---|---|---|
| Johnston et al. 2025 | Maze Engineers Rotarod Instrument | “Rotarod testing was recorded utilizing Maze Engineers Rotarod Instrument (Skokie, IL, USA)” |
| Wen et al. 2024 | Accelerating rotarod IITC Life Science | “the accelerating rotarod (IITC Life Science, Woodland Hills, CA)” |
| Ahire et al. 2023 | Automated four‐lane rotarod tool | “Motor coordination was assessed using an automated four‐lane rotarod tool.” |
| Trabjerg et al. 2023 | Rotamex-5 Rota Rod Columbus Instruments | “Motor function was assessed using a rotarod (Rotamex-5 Rota Rod, Columbus Instruments, Columbus, Ohio, USA)” |
| Somebang et al. 2021 | Rotarod TSE Systems | “Rotarod (Hong et al., 2007, Hsieh et al., 2014, Liu et al., 2007, Raber et al., 2004, Suh et al., 2005) (TSE Systems)” |
| Trabjerg et al. 2021 | Rotamex-5 RotaRod Columbus Instruments | “Rotarod test (Rotamex-5 RotaRod, Columbus Instruments, Columbus, Ohio, USA)” |
| Drapeau et al. 2018 | Rotarod Omnitech Electronics Inc | “was assessed in the Rotarod test (Omnitech Electronics Inc)” |
| Xin et al. 2017 | Accelerating rotarod Softmaze, XR1514 | “The accelerating rotarod [35, 36] (Softmaze-XR1514, Shanghai, China) consisted of a felt-covered steel cylinder fitted to a rotating pump with a variable speed setting.” |
| Florez-Paz et al. 2016 | Rotarod Ugo Basile | “In the rotarod test (Ugo Basile), the mice were placed on a 5 cm diameter rotating cylinder along which they must walk in order not to fall off. The initial speed of rotation was 5 rpm and it accelerated to 20 rpm.” |
No apparatus named in Rattner et al. 2020.