Trabjerg MS, Andersen DC, Huntjens P, et al. NPJ Parkinson's disease 2023;9(1):6.
The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made
| Setting | Value | Paper’s words |
|---|---|---|
| Beam size |
| “the first being with a width of 16 mm at the broadest and narrowing down to 12 mm” “the second with a width of 6 mm” “transverse the 0.6 cm beam” “compared to Wt+R+V at day 42” |
| Beam height, number of trials |
| “The test was conducted according to the protocol^105” |
| Other settings in the paper | ||
| Beams | two | “Mice had to traverse two different beams” |
| Trained until | Prior to the challenging transversal beam test they were able to spontaneously cross the beam | “Prior to the challenging transversal beam test, the mice were trained until they were able to spontaneously cross the beam” |
| Data were excluded if | the mice turned around continuously or walked in the wrong direction | “Data were excluded if the mice turned around continuously or walked in the wrong direction” |
| Beam test | Chronic rotenone mouse models day 42 | “beam test at day 42” |
| Were defined by the presence of | At day 32 a minimum two of the following factors | “At day 32, animals, who did not show signs of disease, were defined by the presence of a minimum two of the following factors: lower grip strength, lower latency to fall on the rotarod, longer time to transverse the beam, pole or lower number of rears in the cylinder test” |
| Video material was rated by | Depending on the experiment three to four blinded individuals | “Video material was rated by three to four blinded individuals, depending on the experiment” |
| Animals were excluded from behavioral tests if | the video camera stopped recording before the test was done | “Animals were excluded from behavioral tests if the video camera stopped recording before the test was done” |
| Mice were acclimatized to the laboratory | 30 min before tests began | “mice were acclimatized to the laboratory 30 min before tests began” |
| All tests were performed | between 10:00 and 16:00 in the lights-on cycle | “All tests were performed between 10:00 and 16:00 in the lights-on cycle” |
| Full cleaning protocol | Between every test 70% ethanol | “Between every test, a full cleaning protocol by 70% ethanol was applied to the surfaces of the equipment” |
| Exposed to behavioral tests | in a randomized order | “The mice were exposed to behavioral tests in a randomized order” |
| Experimenters | blinded to treatment groups | “The experimenters were blinded to treatment groups for all behavioral studies” |
| Apparatus | ||
| Beams | “Mice had to traverse two different beams” | |
| Video camera | “A video camera was used to record each trial” | |
| Methods cited to other work | ||
| Conduct of the test, including settings not printed such as beam height and trial count | ^105 | |
| Setting | Value | Paper’s words |
|---|---|---|
| Number of trials |
| “Each animal received five trials” |
| Other settings in the paper | ||
| Gently pulling the animal | by the tail | “gently pulling the animal by the tail” |
| Data were excluded when | the animal was making movements during the procedure or having an insufficient grip | “Data were excluded when the animal was making movements during the procedure or having an insufficient grip” |
| Data were excluded if | the mice were unable to grasp the grid due to no interest | “Data were excluded if the mice were unable to grasp the grid due to no interest according to the protocol” |
| Mice were evaluated | C57Bl/6J rotenone washout etomoxir experiment at day 54 | “Mice were evaluated for pathological motor and non-motor behavior at day 54 using grip strength, Y-maze, and dark-light box test” |
| Mice were evaluated | Park2 etomoxir experiment At day 22 | “At day 22, mice were evaluated for pathological motor and non-motor behavior using rotarod, grip strength, Y-maze, novel object recognition, and dark-light box test” |
| Mice were evaluated | Park2 rotenone washout etomoxir experiment At day 54 | “At day 54, mice were evaluated for pathological motor and non-motor behavior using rotarod, grip strength, Y-maze, and novel object recognition” |
| Were defined by the presence of | At day 32 a minimum two of the following factors | “At day 32, animals, who did not show signs of disease, were defined by the presence of a minimum two of the following factors: lower grip strength, lower latency to fall on the rotarod, longer time to transverse the beam, pole or lower number of rears in the cylinder test” |
| Mice were acclimatized to the laboratory | 30 min before tests began | “mice were acclimatized to the laboratory 30 min before tests began” |
| All tests were performed | between 10:00 and 16:00 in the lights-on cycle | “All tests were performed between 10:00 and 16:00 in the lights-on cycle” |
| Full cleaning protocol | Between every test 70% ethanol | “Between every test, a full cleaning protocol by 70% ethanol was applied to the surfaces of the equipment” |
| Exposed to behavioral tests | in a randomized order | “The mice were exposed to behavioral tests in a randomized order” |
| Experimenters | blinded to treatment groups | “The experimenters were blinded to treatment groups for all behavioral studies” |
| Apparatus | ||
| Grip strength apparatus | BIOSEB, BIO-GS3 | “Muscle strength assessment was measured using a grip strength apparatus (BIOSEB, model: BIO-GS3, Chaville, France)” |
| Setting | Value | Paper’s words |
|---|---|---|
| Rod speed |
| “The speed was increased from 5 to 40 RPM within 140 s for the C57Bl/6J rotenone etomoxir experiment” “The speed was optimized for the other experiments” “Starting at 5 RPM and ending at 40 RPM at 140 s” |
| Trial duration |
| “The cut-off time was set to 180 s” |
| Number of trials |
| “Each animal received three trials per session” “Prior to the rotarod performance test, mice were trained on three different dates with three trials per session” |
| Other settings in the paper | ||
| Speed was increased | C57Bl/6J rotenone etomoxir experiment within 140 s | “The speed was increased from 5 to 40 RPM within 140 s for the C57Bl/6J rotenone etomoxir experiment” |
| Acceleration | The other experiments 2.5 RPM per 10 s | “The speed was optimized for the other experiments, using an acceleration of 2.5 RPM per 10 s” |
| Ending at 40 RPM | The other experiments at 140 s | “Starting at 5 RPM and ending at 40 RPM at 140 s” |
| Mice were trained on | Prior to the rotarod performance test three different dates | “Prior to the rotarod performance test, mice were trained on three different dates with three trials per session” |
| Data were excluded if | the mice continuously jumped off the rotarod or turned in the wrong direction before acceleration | “Data were excluded if the mice continuously jumped off the rotarod or turned in the wrong direction before acceleration” |
| Motor test | Chronic rotenone mouse models day 60 | “motor test at day 60 (rotarod)” |
| Mice were evaluated | Park2 etomoxir experiment At day 22 | “At day 22, mice were evaluated for pathological motor and non-motor behavior using rotarod, grip strength, Y-maze, novel object recognition, and dark-light box test” |
| Mice were evaluated | Park2 rotenone washout etomoxir experiment At day 54 | “At day 54, mice were evaluated for pathological motor and non-motor behavior using rotarod, grip strength, Y-maze, and novel object recognition” |
| Were defined by the presence of | At day 32 a minimum two of the following factors | “At day 32, animals, who did not show signs of disease, were defined by the presence of a minimum two of the following factors: lower grip strength, lower latency to fall on the rotarod, longer time to transverse the beam, pole or lower number of rears in the cylinder test” |
| Mice were acclimatized to the laboratory | 30 min before tests began | “mice were acclimatized to the laboratory 30 min before tests began” |
| All tests were performed | between 10:00 and 16:00 in the lights-on cycle | “All tests were performed between 10:00 and 16:00 in the lights-on cycle” |
| Full cleaning protocol | Between every test 70% ethanol | “Between every test, a full cleaning protocol by 70% ethanol was applied to the surfaces of the equipment” |
| Exposed to behavioral tests | in a randomized order | “The mice were exposed to behavioral tests in a randomized order” |
| Experimenters | blinded to treatment groups | “The experimenters were blinded to treatment groups for all behavioral studies” |
| Apparatus | ||
| Rotamex-5 Rota Rod | Columbus Instruments | “Motor function was assessed using a rotarod (Rotamex-5 Rota Rod, Columbus Instruments, Columbus, Ohio, USA)” |
| Setting | Value | Paper’s words |
|---|---|---|
| Session duration |
| “Mice were placed in the maze for 5 min” |
| Arm size |
| “Y-maze was constructed according to MazeEngineers (USA) description” |
| Other settings in the paper | ||
| Blinded raters | three | “the video was evaluated by three blinded raters” |
| Data were excluded if | the mice did not conduct a minimum of predefined number of entries | “Data were excluded if the mice did not conduct a minimum of predefined number of entries according to the protocol” |
| Mice were evaluated | C57Bl/6J rotenone washout etomoxir experiment at day 54 | “Mice were evaluated for pathological motor and non-motor behavior at day 54 using grip strength, Y-maze, and dark-light box test” |
| Mice were evaluated | Park2 etomoxir experiment At day 22 | “At day 22, mice were evaluated for pathological motor and non-motor behavior using rotarod, grip strength, Y-maze, novel object recognition, and dark-light box test” |
| Mice were evaluated | Park2 rotenone washout etomoxir experiment At day 54 | “At day 54, mice were evaluated for pathological motor and non-motor behavior using rotarod, grip strength, Y-maze, and novel object recognition” |
| Animals were excluded from behavioral tests if | the video camera stopped recording before the test was done | “Animals were excluded from behavioral tests if the video camera stopped recording before the test was done” |
| Mice were acclimatized to the laboratory | 30 min before tests began | “mice were acclimatized to the laboratory 30 min before tests began” |
| All tests were performed | between 10:00 and 16:00 in the lights-on cycle | “All tests were performed between 10:00 and 16:00 in the lights-on cycle” |
| Full cleaning protocol | Between every test 70% ethanol | “Between every test, a full cleaning protocol by 70% ethanol was applied to the surfaces of the equipment” |
| Exposed to behavioral tests | in a randomized order | “The mice were exposed to behavioral tests in a randomized order” |
| Experimenters | blinded to treatment groups | “The experimenters were blinded to treatment groups for all behavioral studies” |
| Apparatus | ||
| Y-maze | MazeEngineers | “Y-maze was constructed according to MazeEngineers (USA) description” |
| Video camera | “A video camera was used to record each trial” | |
| Methods cited to other work | ||
| Conduct of the Y-maze test | ^107 | “was done as previously described^107” |
| Construction of the maze, including arm dimensions | MazeEngineers (USA) description | |