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Tests/Y-maze test

Y-maze test

How 8 papers set up this test.

AI read these papers and code checked every quote. No person has reviewed the records yet. How records are made

Arm size

Length, width and wall height of each maze arm

PaperValuePaper’s words
Vanderplow et al. 2022Rats
  • 11-cm wide and 50-cm long

“with each arm 11-cm wide and 50-cm long”
Rattner et al. 2020Mice
  • 38 cm-long

“The Y-maze consists of three 38 cm-long arms”
Drapeau et al. 2018Mice
  • 5-cm-wide and 50-cm-long

“three 5-cm-wide and 50-cm-long arms”
Ahire et al. 2023Mice
  • Cited to other work

    No arm dimensions are printed. The Y‐maze procedure is described only by reference to the authors' published protocol.

“according to our published protocol (Csiszar et al., 2013)”
Li et al. 2023Mice
  • Cited to other work

    No arm size is printed. The pointer covers the method as a whole and does not name arm size.

“The Y-maze test was carried out based on the method previously described [23]”
Trabjerg et al. 2023Mice
  • Cited to other work

    No arm dimensions are printed; the passage sends the reader to the MazeEngineers description.

“Y-maze was constructed according to MazeEngineers (USA) description”
Trabjerg et al. 2021Mice
  • Cited to other work

    No arm dimensions are printed. The passage sends the reader to Maze Engineers for how the maze was constructed.

“The y-maze test was constructed according to Maze Engineers (USA)”

Not in the Methods of Wanner et al. 2021.

Session duration

How long one session in the apparatus lasts

PaperValuePaper’s words
Li et al. 2023Mice
  • 5 min

“allowed to freely explore the maze for 5 min”
Trabjerg et al. 2023Mice
  • 5 min

“Mice were placed in the maze for 5 min”
Vanderplow et al. 2022Rats
  • 10 minutes

“subsequent arm choices analyzed for 10 minutes thereafter”
Trabjerg et al. 2021Mice
  • 5 min

“The mice were placed in the y-maze for 5 min”
Wanner et al. 2021Mice
  • ten minutes

“recorded on video using a tripod and digital camera for ten minutes”
“After ten minutes, the animal was returned to the home cage”
Rattner et al. 2020Mice
  • Training phase

    5 min

  • Test phase

    5 min

“The mouse was placed at the end of one of the two open arms and allowed to explore for 5 min”
“the test phase began: the blockade was removed, and the mouse was allowed to explore all three arms of the maze for 5 min”
Drapeau et al. 2018Mice
  • 15 min

“given 15 min to freely explore the three arms of the maze”
Ahire et al. 2023Mice
  • Cited to other work

    No session duration is printed. The Y‐maze procedure is described only by reference to the authors' published protocol.

“according to our published protocol (Csiszar et al., 2013)”

Other settings the papers report (61)

Settings outside the list above, named as each paper names them.

PaperSetting and valuePaper’s words
Drapeau et al. 2018Mice

A successful score

three successive choices that includes one instance of each arm

“A successful score is defined by three successive choices that includes one instance of each arm”
Drapeau et al. 2018Mice

A type 1 error

three consecutive choices where the first and third choices are identical

“A type 1 error is determined by three consecutive choices where the first and third choices are identical”
Drapeau et al. 2018Mice

A type 2 error

three consecutive choices where the second and third choices are identical

“A type 2 error is defined by three consecutive choices where the second and third choices are identical”
Trabjerg et al. 2023Mice

All tests were performed

between 10:00 and 16:00 in the lights-on cycle

“All tests were performed between 10:00 and 16:00 in the lights-on cycle”
Li et al. 2023Mice

An arm entry was counted

when all four limbs of the mouse were within an arm

“An arm entry was counted when all four limbs of the mouse were within an arm”
Drapeau et al. 2018Mice

An entry occurs when

all four limbs are within the arm

“An entry occurs when all four limbs are within the arm”
Vanderplow et al. 2022Rats

Analysis of arm entries

began after the animal entered each arm once

“Analysis of arm entries began after the animal entered each arm once”
Trabjerg et al. 2023Mice

Animals were excluded from behavioral tests if

the video camera stopped recording before the test was done

“Animals were excluded from behavioral tests if the video camera stopped recording before the test was done”
Wanner et al. 2021Mice

Apparatus was sanitized

70% alcohol

“the apparatus was sanitized with 70% alcohol”
Li et al. 2023Mice

Arm designated

(A)

“each mouse was placed in the arm designated (A) of the Y-maze”
Trabjerg et al. 2021Mice

Arms

three

“to freely explore the three arms”
Wanner et al. 2021Mice

Arms

three

“three arms connected at 120°”
Rattner et al. 2020Mice

Arms

three

“The Y-maze consists of three 38 cm-long arms”
Drapeau et al. 2018Mice

Arms

three

“three 5-cm-wide and 50-cm-long arms”
Wanner et al. 2021Mice

Arms connected at

120°

“three arms connected at 120°”
Wanner et al. 2021Mice

Behavior tests, F1

once per animal

“the same behavior tests once per animal”
Drapeau et al. 2018Mice

Behavioral experiments were conducted

between 9 A.M. and 5 P.M.

“Behavioral experiments were conducted between 9 A.M. and 5 P.M.”
Wanner et al. 2021Mice

Between tests, F1

at least 24 h

“with at least 24 h between tests”
Trabjerg et al. 2023Mice

Blinded raters

three

“the video was evaluated by three blinded raters”
Trabjerg et al. 2021Mice

Blinded raters

four

“the number of entries and triplets were noted by four blinded raters”
Rattner et al. 2020Mice

Blocked, training phase

one arm

“During the training phase, one arm of the Y-maze was blocked”
Wanner et al. 2021Mice

Chamber

high-walled

“a high-walled chamber”
Trabjerg et al. 2021Mice

Cleaned with

70% ethanol between each animal

“Test equipment was cleaned with 70% ethanol between each animal”
Wanner et al. 2021Mice

Consecutive tests, F0

at least 24 h apart

“with consecutive tests being at least 24 h apart”
Vanderplow et al. 2022Rats

Criteria for arm entry

hind paws completely within a maze arm

“criteria for arm entry included an animal having its hind paws completely within a maze arm”
Vanderplow et al. 2022Rats

Cues

distal spatial cues

“which was surrounded by distal spatial cues”
Trabjerg et al. 2023Mice

Data were excluded if

the mice did not conduct a minimum of predefined number of entries

“Data were excluded if the mice did not conduct a minimum of predefined number of entries according to the protocol”
Ahire et al. 2023Mice

Delayed alternation task

two‐trial

“Y‐maze two‐trial delayed alternation task”
Drapeau et al. 2018Mice

Each set

130°

“each set 130° from each other”
Wanner et al. 2021Mice

Entries

all four feet within the arm

“The sequence of entries (all four feet within the arm)”
Trabjerg et al. 2021Mice

Evaluated, CPT1A antagonist studies

day 70, day 100 and day 130

“at day 70, day 100 and day 130 mice were evaluated by cylinder, and y-maze test”
Trabjerg et al. 2021Mice

Evaluated, SOD1Wt/Cpt1a and SOD1Cpt1a/Cpt1a studies

day 70, 100, and 130

“mice were evaluated at day 70, 100, and 130 using cylinder (n = 7–11), and y-maze test (n = 6–9)”
Trabjerg et al. 2021Mice

Evaluated, SOD1 G93A High-fat diet study

day 70, and day 130

“mice were evaluated at day 70, and day 130 using cylinder, and y-maze test”
Trabjerg et al. 2021Mice

Evaluated, SOD1 G93A corticosterone study

day 70, and day 100

“mice were evaluated at day 70, and day 100 using cylinder and y-maze test”
Trabjerg et al. 2021Mice

Evaluated, SOD1 males (n = 9) and females (n = 5)

day 140

“SOD1 males (n = 9) and females (n = 5) were evaluated by neurological score, grip strength, hangwire test and y-maze test at day 140”
Vanderplow et al. 2022Rats

Experimenter

blind to conditions

“The test was carried out by, and analyzed by, an experimenter blind to conditions.”
Trabjerg et al. 2023Mice

Experimenters

blinded to treatment groups

“The experimenters were blinded to treatment groups for all behavioral studies”
Rattner et al. 2020Mice

Experimenters

blind to the genotype

“During all behavioral testing, the experimenters were blind to the genotype”
Trabjerg et al. 2023Mice

Exposed to behavioral tests

in a randomized order

“The mice were exposed to behavioral tests in a randomized order”
Wanner et al. 2021Mice

Following the weaning of pups, F0

between 4–7 days

“between 4–7 days following the weaning of pups”
Trabjerg et al. 2023Mice

Full cleaning protocol, Between every test

70% ethanol

“Between every test, a full cleaning protocol by 70% ethanol was applied to the surfaces of the equipment”
Rattner et al. 2020Mice

Inter-trial interval

30-min

“After a 30-min inter-trial interval, the test phase began”
Wanner et al. 2021Mice

Investigator

blinded to treatment group

“recorded from the video by an investigator blinded to treatment group”
Wanner et al. 2021Mice

Investigators

vacated the room

“Investigators vacated the room”
Wanner et al. 2021Mice

Light–dark cycle

light phase

“during the light phase of the light–dark cycle”
Trabjerg et al. 2023Mice

Mice were acclimatized to the laboratory

30 min before tests began

“mice were acclimatized to the laboratory 30 min before tests began”
Drapeau et al. 2018Mice

Mice were brought to the front room of the testing area

at least half an hour

“Mice were brought to the front room of the testing area at least half an hour before the start of experiments”
Trabjerg et al. 2023Mice

Mice were evaluated, C57Bl/6J rotenone washout etomoxir experiment

at day 54

“Mice were evaluated for pathological motor and non-motor behavior at day 54 using grip strength, Y-maze, and dark-light box test”
Trabjerg et al. 2023Mice

Mice were evaluated, Park2 etomoxir experiment

At day 22

“At day 22, mice were evaluated for pathological motor and non-motor behavior using rotarod, grip strength, Y-maze, novel object recognition, and dark-light box test”
Trabjerg et al. 2023Mice

Mice were evaluated, Park2 rotenone washout etomoxir experiment

At day 54

“At day 54, mice were evaluated for pathological motor and non-motor behavior using rotarod, grip strength, Y-maze, and novel object recognition”
Wanner et al. 2021Mice

Mice were placed

at the end of one arm facing the center

“Mice were placed at the end of one arm facing the center”
Wanner et al. 2021Mice

One spontaneous alternation

three consecutive entries into unique arms

“One spontaneous alternation was counted when three consecutive entries into unique arms (e.g., A, B, C) were recorded”
Rattner et al. 2020Mice

Percent time spent in the novel arm, test phase

first 2 min

“Data from the first 2 min of the test phase were used to evaluate percent time spent in the novel arm”
Trabjerg et al. 2021Mice

Performed

between 9 am and 2 pm

“All clinical behavioral tests were performed between 9 am and 2 pm”
Drapeau et al. 2018Mice

Perseverance

three or more repetitive entries in the same arm

“Perseverance is defined as three or more repetitive entries in the same arm”
Trabjerg et al. 2021Mice

Placed in the room

one hour before test sessions

“mice were placed in the room one hour before test sessions to allow time for acclimatization”
Vanderplow et al. 2022Rats

Removed from analysis

did not make at least 10 arm choice during the 10-minute period

“Any rats that did not make at least 10 arm choice during the 10-minute period were removed from analysis”
Wanner et al. 2021Mice

Tested, F0

twice

“Each F0 female was tested twice”
Wanner et al. 2021Mice

Tests were conducted in

the home mouse room

“Tests were conducted in the home mouse room”
Rattner et al. 2020Mice

The mouse was placed, training phase

at the end of one of the two open arms

“The mouse was placed at the end of one of the two open arms”
Vanderplow et al. 2022Rats

The order from first to last

Y-maze, open field, novel object recognition, social interaction, and social novelty

“with the order from first to last as the following: Y-maze, open field, novel object recognition, social interaction, and social novelty”

Apparatus

What each paper names for this test. Vendors and models appear as the paper prints them.

PaperApparatusPaper’s words
Ahire et al. 2023

Y‐maze

“Hippocampal‐dependent contextual memory was tested with the Y‐maze two‐trial delayed alternation task”
Li et al. 2023

Y-maze

“each mouse was placed in the arm designated (A) of the Y-maze”
Trabjerg et al. 2023

Y-maze

MazeEngineers

“Y-maze was constructed according to MazeEngineers (USA) description”
Trabjerg et al. 2023

Video camera

“A video camera was used to record each trial”
Vanderplow et al. 2022

Y-maze

Maze Engineers (Skokie, IL, USA)

“The Y-maze was purchased from Maze Engineers (Skokie, IL, USA)”
Vanderplow et al. 2022

Overhead digital video camera

“All animal behaviors were videotaped with an overhead digital video camera.”
Trabjerg et al. 2021

Y-maze

Maze Engineers

“The y-maze test was constructed according to Maze Engineers (USA)”
Trabjerg et al. 2021

Video

“The y-maze test was recorded by video”
Wanner et al. 2021

Standard Y-maze

MazeEngineers

“a standard Y-maze (MazeEngineers, Boston, MA)”
Wanner et al. 2021

Tripod

“recorded on video using a tripod and digital camera”
Wanner et al. 2021

Digital camera

“recorded on video using a tripod and digital camera”
Rattner et al. 2020

Y-maze

San Diego Instruments

“The Y-maze consists of three 38 cm-long arms (San Diego Instruments)”
Rattner et al. 2020

Topscan tracking software

Cleversys

“Distance traveled and time spent in each arm was automatically recorded using Topscan tracking software (Cleversys)”
Drapeau et al. 2018

Y-maze

“Mice were placed in the center of a Y-maze”