Rattner A, Terrillion CE, Jou C, et al. eLife 2020;9:e62766.
The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made
| Setting | Value | Paper’s words |
|---|---|---|
| Not in the Methods | Arm size, maze elevation, session duration, illumination | |
| Other settings in the paper | ||
| Experimenters | blind to the genotype | “During all behavioral testing, the experimenters were blind to the genotype” |
| Setting | Value | Paper’s words |
|---|---|---|
| Pool size |
| “The maze consisted of a circular stainless-steel tank 4 m in diameter” |
| Water temperature |
| “filled with room temperature water made opaque with white tempera paint for the training, probe, and reversal trials” |
| Platform size |
| “During the pretraining phase, a 10 cm platform was placed 1 cm below clear water” “During the hidden platform training phase, a 10 cm platform was placed in a fixed location in the maze” |
| Platform depth below the surface |
| “During the pretraining phase, a 10 cm platform was placed 1 cm below clear water” “with the top of the platform hidden beneath 1 cm of water” |
| Trial duration |
| “given 60 s to escape onto the visible platform” “The mouse was allowed to search for the platform for 60 s, and, after finding the platform” “the mouse was tested in one 60 s probe trial” “The mouse was allowed to search for the platform for 60 s and, after finding the platform” |
| Number of trials |
| “throughout six trials in one day” “throughout six trials each day for three consecutive days” “the mouse was tested in one 60 s probe trial” “throughout six trials each day for two consecutive days” |
| Other settings in the paper | ||
| Stainless-steel cylinder | Pretraining phase 2-m diameter | “in the center of a 2-m diameter stainless-steel cylinder in the MWM” |
| Following the hidden platform training phase | Probe trial 24 hr | “24 hr following the hidden platform training phase, the mouse was tested in one 60 s probe trial” |
| To remain there | Hidden platform training phase 15 s | “for 60 s, and, after finding the platform, to remain there for 15 s” |
| To remain there | Reversal phase 15 s | “for 60 s and, after finding the platform, to remain there for 15 s” |
| Start positions in a semi-random fashion | Hidden platform training phase four | “in one of four start positions in a semi-random fashion throughout six trials each day for three consecutive days” |
| Start positions in a semi-random fashion | Reversal phase four | “in one of four start positions in a semi-random fashion throughout six trials each day for two consecutive days” |
| The mouse was placed | Pretraining phase inside the perimeter of the cylinder | “The mouse was placed inside the perimeter of the cylinder” |
| Day | Pretraining phase one day | “throughout six trials in one day” |
| Consecutive days | Hidden platform training phase three consecutive days | “throughout six trials each day for three consecutive days” |
| Consecutive days | Reversal phase two consecutive days | “throughout six trials each day for two consecutive days” |
| Platform was placed | Pretraining phase in the center of a 2-m diameter stainless-steel cylinder | “a 10 cm platform was placed 1 cm below clear water in the center of a 2-m diameter stainless-steel cylinder” |
| Platform was placed | Hidden platform training phase in a fixed location in the maze | “a 10 cm platform was placed in a fixed location in the maze” |
| Platform was placed | Reversal phase in a different quadrant from that used during the training phase | “the hidden platform was placed in the MWM in a different quadrant from that used during the training phase” |
| Water | Training, probe, and reversal trials opaque with white tempera paint | “water made opaque with white tempera paint for the training, probe, and reversal trials” |
| Water | Pretraining phase clear | “placed 1 cm below clear water” |
| Circle centered on the platform | 8 cm diameter | “crossing an 8 cm diameter circle centered on the platform” |
| Experimenters | blind to the genotype | “During all behavioral testing, the experimenters were blind to the genotype” |
| Apparatus | ||
| White tempera paint | “water made opaque with white tempera paint” | |
| Anymaze tracking software | Stoelting | “time spent in the platform quadrant were measured using Anymaze tracking software (Stoelting)” |
| Circular stainless-steel tank | “The maze consisted of a circular stainless-steel tank 4 m in diameter” | |
| Stainless-steel cylinder | “in the center of a 2-m diameter stainless-steel cylinder in the MWM” | |
| Platform | “a 10 cm platform was placed in a fixed location in the maze” | |
| Methods cited to other work | ||
| Morris water maze protocol | Pletnikov et al., 2008 | “The Morris water maze test (MWM) was based on the protocol previously described in Pletnikov et al., 2008” |
| Setting | Value | Paper’s words |
|---|---|---|
| Not in the Methods | Arena size, session duration, illumination | |
| Other settings in the paper | ||
| Experimenters | blind to the genotype | “During all behavioral testing, the experimenters were blind to the genotype” |
| Setting | Value | Paper’s words |
|---|---|---|
| Not in the Methods | Rod speed, trial duration, number of trials | |
| Other settings in the paper | ||
| Experimenters | blind to the genotype | “During all behavioral testing, the experimenters were blind to the genotype” |
| Setting | Value | Paper’s words |
|---|---|---|
| Session duration |
| “The mouse was placed at the end of one of the two open arms and allowed to explore for 5 min” “the test phase began: the blockade was removed, and the mouse was allowed to explore all three arms of the maze for 5 min” |
| Arm size |
| “The Y-maze consists of three 38 cm-long arms” |
| Other settings in the paper | ||
| Inter-trial interval | 30-min | “After a 30-min inter-trial interval, the test phase began” |
| Arms | three | “The Y-maze consists of three 38 cm-long arms” |
| Blocked | Training phase one arm | “During the training phase, one arm of the Y-maze was blocked” |
| The mouse was placed | Training phase at the end of one of the two open arms | “The mouse was placed at the end of one of the two open arms” |
| Percent time spent in the novel arm | Test phase first 2 min | “Data from the first 2 min of the test phase were used to evaluate percent time spent in the novel arm” |
| Experimenters | blind to the genotype | “During all behavioral testing, the experimenters were blind to the genotype” |
| Apparatus | ||
| Y-maze | San Diego Instruments | “The Y-maze consists of three 38 cm-long arms (San Diego Instruments)” |
| Topscan tracking software | Cleversys | “Distance traveled and time spent in each arm was automatically recorded using Topscan tracking software (Cleversys)” |