Developmental cannabidiol exposure increases anxiety and modifies genome-wide brain DNA methylation in adult female mice

Wanner NM, Colwell M, Drown C, et al. Clinical epigenetics 2021;13(1):4.

The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made

Marble burying test

Setting	Value	Paper’s words
Session duration	30 min	“Testing was conducted with the investigator absent from the room for 30 min” “After 30 min, mice were carefully removed from the test cage”
Other settings in the paper
Cage	rat cage	“Mice were individually placed into the corner of a rat cage”
Mice were individually placed into	the corner	“Mice were individually placed into the corner of a rat cage”
Corn cob bedding	10 cm (height)	“filled with 10 cm (height) of corn cob bedding”
Marbles	20	“20 evenly placed marbles (4 × 5 layout)”
Layout	4 × 5	“20 evenly placed marbles (4 × 5 layout)”
Investigator	absent from the room	“with the investigator absent from the room”
Counted as buried	at least 2/3 covered	“marbles at least 2/3 covered being counted as buried”
Bedding	thoroughly mixed	“The bedding was thoroughly mixed”
Marbles were	washed with dish soap and rinsed with 70% ethanol	“marbles were washed with dish soap and rinsed with 70% ethanol following each trial”
Tests were conducted in	the home mouse room	“Tests were conducted in the home mouse room”
Light–dark cycle	light phase	“during the light phase of the light–dark cycle”
Following the weaning of pups	F0 between 4–7 days	“between 4–7 days following the weaning of pups”
Tested	F0 twice	“Each F0 female was tested twice”
Behavior tests	F1 once per animal	“the same behavior tests once per animal”
Consecutive tests	F0 at least 24 h apart	“with consecutive tests being at least 24 h apart”
Between tests	F1 at least 24 h	“with at least 24 h between tests”
Apparatus
Rat cage		“Mice were individually placed into the corner of a rat cage”
Corn cob bedding		“filled with 10 cm (height) of corn cob bedding”
Marbles		“20 evenly placed marbles (4 × 5 layout)”

Y-maze test

Compare with 7 other papers

Setting	Value	Paper’s words
Session duration	ten minutes	“recorded on video using a tripod and digital camera for ten minutes” “After ten minutes, the animal was returned to the home cage”
Other settings in the paper
Arms	three	“three arms connected at 120°”
Arms connected at	120°	“three arms connected at 120°”
Chamber	high-walled	“a high-walled chamber”
Mice were placed	at the end of one arm facing the center	“Mice were placed at the end of one arm facing the center”
Investigators	vacated the room	“Investigators vacated the room”
Apparatus was sanitized	70% alcohol	“the apparatus was sanitized with 70% alcohol”
Entries	all four feet within the arm	“The sequence of entries (all four feet within the arm)”
One spontaneous alternation	three consecutive entries into unique arms	“One spontaneous alternation was counted when three consecutive entries into unique arms (e.g., A, B, C) were recorded”
Investigator	blinded to treatment group	“recorded from the video by an investigator blinded to treatment group”
Tests were conducted in	the home mouse room	“Tests were conducted in the home mouse room”
Light–dark cycle	light phase	“during the light phase of the light–dark cycle”
Following the weaning of pups	F0 between 4–7 days	“between 4–7 days following the weaning of pups”
Tested	F0 twice	“Each F0 female was tested twice”
Behavior tests	F1 once per animal	“the same behavior tests once per animal”
Consecutive tests	F0 at least 24 h apart	“with consecutive tests being at least 24 h apart”
Between tests	F1 at least 24 h	“with at least 24 h between tests”
Apparatus
Standard Y-maze	MazeEngineers	“a standard Y-maze (MazeEngineers, Boston, MA)”
Tripod		“recorded on video using a tripod and digital camera”
Digital camera		“recorded on video using a tripod and digital camera”

Developmental cannabidiol exposure increases anxiety and modifies genome-wide brain DNA methylation in adult female mice

Wanner NM, Colwell M, Drown C, et al. Clinical epigenetics 2021;13(1):4.

The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made

Marble burying test

Compare with 2 other papers

Setting	Value	Paper’s words
Session duration	30 min	“Testing was conducted with the investigator absent from the room for 30 min” “After 30 min, mice were carefully removed from the test cage”
Other settings in the paper
Cage	rat cage	“Mice were individually placed into the corner of a rat cage”
Mice were individually placed into	the corner	“Mice were individually placed into the corner of a rat cage”
Corn cob bedding	10 cm (height)	“filled with 10 cm (height) of corn cob bedding”
Marbles	20	“20 evenly placed marbles (4 × 5 layout)”
Layout	4 × 5	“20 evenly placed marbles (4 × 5 layout)”
Investigator	absent from the room	“with the investigator absent from the room”
Counted as buried	at least 2/3 covered	“marbles at least 2/3 covered being counted as buried”
Bedding	thoroughly mixed	“The bedding was thoroughly mixed”
Marbles were	washed with dish soap and rinsed with 70% ethanol	“marbles were washed with dish soap and rinsed with 70% ethanol following each trial”
Tests were conducted in	the home mouse room	“Tests were conducted in the home mouse room”
Light–dark cycle	light phase	“during the light phase of the light–dark cycle”
Following the weaning of pups	F0 between 4–7 days	“between 4–7 days following the weaning of pups”
Tested	F0 twice	“Each F0 female was tested twice”
Behavior tests	F1 once per animal	“the same behavior tests once per animal”
Consecutive tests	F0 at least 24 h apart	“with consecutive tests being at least 24 h apart”
Between tests	F1 at least 24 h	“with at least 24 h between tests”
Apparatus
Rat cage		“Mice were individually placed into the corner of a rat cage”
Corn cob bedding		“filled with 10 cm (height) of corn cob bedding”
Marbles		“20 evenly placed marbles (4 × 5 layout)”

Y-maze test

Compare with 7 other papers

Setting	Value	Paper’s words
Session duration	ten minutes	“recorded on video using a tripod and digital camera for ten minutes” “After ten minutes, the animal was returned to the home cage”
Other settings in the paper
Arms	three	“three arms connected at 120°”
Arms connected at	120°	“three arms connected at 120°”
Chamber	high-walled	“a high-walled chamber”
Mice were placed	at the end of one arm facing the center	“Mice were placed at the end of one arm facing the center”
Investigators	vacated the room	“Investigators vacated the room”
Apparatus was sanitized	70% alcohol	“the apparatus was sanitized with 70% alcohol”
Entries	all four feet within the arm	“The sequence of entries (all four feet within the arm)”
One spontaneous alternation	three consecutive entries into unique arms	“One spontaneous alternation was counted when three consecutive entries into unique arms (e.g., A, B, C) were recorded”
Investigator	blinded to treatment group	“recorded from the video by an investigator blinded to treatment group”
Tests were conducted in	the home mouse room	“Tests were conducted in the home mouse room”
Light–dark cycle	light phase	“during the light phase of the light–dark cycle”
Following the weaning of pups	F0 between 4–7 days	“between 4–7 days following the weaning of pups”
Tested	F0 twice	“Each F0 female was tested twice”
Behavior tests	F1 once per animal	“the same behavior tests once per animal”
Consecutive tests	F0 at least 24 h apart	“with consecutive tests being at least 24 h apart”
Between tests	F1 at least 24 h	“with at least 24 h between tests”
Apparatus
Standard Y-maze	MazeEngineers	“a standard Y-maze (MazeEngineers, Boston, MA)”
Tripod		“recorded on video using a tripod and digital camera”
Digital camera		“recorded on video using a tripod and digital camera”