Supraphysiologic doses of 17β-estradiol aggravate depression-like behaviors in ovariectomized mice possibly via regulating microglial responses and brain glycerophospholipid metabolism

Li M, Zhang J, Chen W, et al. Journal of neuroinflammation 2023;20(1):204.

The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made

Forced swim test

Setting	Value	Paper’s words
Water temperature	23 °C	“filled with water, and maintained at 23 °C”
Water depth	Cited to other work The buckets are filled with water but no depth is printed. The pointer covers the method as a whole and does not name depth.	“according to the method previously described [24]”
Cylinder size	18 cm in diameter and 25 cm in height	“plastic buckets, each 18 cm in diameter and 25 cm in height”
Session duration	Unclear 5 min The 5 min is the recording period, which follows 1 min of habituation in the bucket. The total time in the water is not printed, so 5 min may not be the full session.	“After 1 min of habituation, the immobility time (in the unit o sec) of the mice were recorded for a period of 5 min”
Other settings in the paper
Habituation	1 min	“After 1 min of habituation”
Buckets	plastic	“plastic buckets”
Apparatus
Plastic buckets		“the mice were placed into plastic buckets”
Methods cited to other work
Forced swim test procedure beyond the brief summary, including the water depth	[24]

Grip strength test

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Setting	Value	Paper’s words

Open field test

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Setting	Value	Paper’s words
Arena size	45 cm × 45 cm × 30 cm	“a white opaque arena of 45 cm × 45 cm × 30 cm in size”
Session duration	5 min	“The open field test consisted of 5 min trial”
Center zone size	Unclear nine square quadrants Time is scored in the central quadrant of an arena divided into nine equal squares, which implies a center of one ninth of the floor, but no size for the center zone is printed.	“The test arena was divided equally into nine square quadrants” “the time spent in the central quadrant of the open field”
Not in the Methods	Illumination
Other settings in the paper
Placed into	the central quadrant of the open field	“Each mouse was placed into the central quadrant of the open field”
Arena	white opaque	“a white opaque arena”
Apparatus
White opaque arena	Maze Engineers, Boston, USA	“a white opaque arena of 45 cm × 45 cm × 30 cm in size (Maze Engineers, Boston, USA)”
Camera		“was recorded by the camera”
ANY-mazeTM video-tracking software	Stoelting Co., Chicago, IL, USA	“analyzed using ANY-mazeTM video-tracking software (Stoelting Co., Chicago, IL, USA)”

Sucrose preference test

Compare with 4 other papers

Setting	Value	Paper’s words
Habituated	3 days	“Mice were habituated to 1% sucrose water for 3 days prior to the test”
Sucrose water	1%	“mice were provided with 1% sucrose water and pure water”
Deprivation of water and food	24 h	“After 24 h deprivation of water and food”
Testing	24 h	“The consumption levels were measured after 24 h testing”

Y-maze test

Compare with 7 other papers

Setting	Value	Paper’s words
Session duration	5 min	“allowed to freely explore the maze for 5 min”
Arm size	Cited to other work No arm size is printed. The pointer covers the method as a whole and does not name arm size.	“The Y-maze test was carried out based on the method previously described [23]”
Other settings in the paper
Arm designated	(A)	“each mouse was placed in the arm designated (A) of the Y-maze”
An arm entry was counted	when all four limbs of the mouse were within an arm	“An arm entry was counted when all four limbs of the mouse were within an arm”
Apparatus
Y-maze		“each mouse was placed in the arm designated (A) of the Y-maze”
Methods cited to other work
Y-maze test procedure beyond the brief summary, including the maze dimensions	[23]

Supraphysiologic doses of 17β-estradiol aggravate depression-like behaviors in ovariectomized mice possibly via regulating microglial responses and brain glycerophospholipid metabolism

Li M, Zhang J, Chen W, et al. Journal of neuroinflammation 2023;20(1):204.

The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made

Forced swim test

Compare with 4 other papers

Setting	Value	Paper’s words
Water temperature	23 °C	“filled with water, and maintained at 23 °C”
Water depth	Cited to other work The buckets are filled with water but no depth is printed. The pointer covers the method as a whole and does not name depth.	“according to the method previously described [24]”
Cylinder size	18 cm in diameter and 25 cm in height	“plastic buckets, each 18 cm in diameter and 25 cm in height”
Session duration	Unclear 5 min The 5 min is the recording period, which follows 1 min of habituation in the bucket. The total time in the water is not printed, so 5 min may not be the full session.	“After 1 min of habituation, the immobility time (in the unit o sec) of the mice were recorded for a period of 5 min”
Other settings in the paper
Habituation	1 min	“After 1 min of habituation”
Buckets	plastic	“plastic buckets”
Apparatus
Plastic buckets		“the mice were placed into plastic buckets”
Methods cited to other work
Forced swim test procedure beyond the brief summary, including the water depth	[24]

Grip strength test

Compare with 6 other papers

Setting	Value	Paper’s words

Open field test

Compare with 10 other papers

Setting	Value	Paper’s words
Arena size	45 cm × 45 cm × 30 cm	“a white opaque arena of 45 cm × 45 cm × 30 cm in size”
Session duration	5 min	“The open field test consisted of 5 min trial”
Center zone size	Unclear nine square quadrants Time is scored in the central quadrant of an arena divided into nine equal squares, which implies a center of one ninth of the floor, but no size for the center zone is printed.	“The test arena was divided equally into nine square quadrants” “the time spent in the central quadrant of the open field”
Not in the Methods	Illumination
Other settings in the paper
Placed into	the central quadrant of the open field	“Each mouse was placed into the central quadrant of the open field”
Arena	white opaque	“a white opaque arena”
Apparatus
White opaque arena	Maze Engineers, Boston, USA	“a white opaque arena of 45 cm × 45 cm × 30 cm in size (Maze Engineers, Boston, USA)”
Camera		“was recorded by the camera”
ANY-mazeTM video-tracking software	Stoelting Co., Chicago, IL, USA	“analyzed using ANY-mazeTM video-tracking software (Stoelting Co., Chicago, IL, USA)”

Sucrose preference test

Compare with 4 other papers

Setting	Value	Paper’s words
Habituated	3 days	“Mice were habituated to 1% sucrose water for 3 days prior to the test”
Sucrose water	1%	“mice were provided with 1% sucrose water and pure water”
Deprivation of water and food	24 h	“After 24 h deprivation of water and food”
Testing	24 h	“The consumption levels were measured after 24 h testing”

Y-maze test

Compare with 7 other papers

Setting	Value	Paper’s words
Session duration	5 min	“allowed to freely explore the maze for 5 min”
Arm size	Cited to other work No arm size is printed. The pointer covers the method as a whole and does not name arm size.	“The Y-maze test was carried out based on the method previously described [23]”
Other settings in the paper
Arm designated	(A)	“each mouse was placed in the arm designated (A) of the Y-maze”
An arm entry was counted	when all four limbs of the mouse were within an arm	“An arm entry was counted when all four limbs of the mouse were within an arm”
Apparatus
Y-maze		“each mouse was placed in the arm designated (A) of the Y-maze”
Methods cited to other work
Y-maze test procedure beyond the brief summary, including the maze dimensions	[23]