Downregulating carnitine palmitoyl transferase 1 affects disease progression in the SOD1 G93A mouse model of ALS

Trabjerg MS, Andersen DC, Huntjens P, et al. Communications biology 2021;4(1):509.

The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made

Grip strength test

Setting	Value	Paper’s words
Number of trials	four trials	“Each mouse received four trials at each session”
Other settings in the paper
Maximum tension	grams	“The maximum tension was measured in grams”
Pulled by	its tail	“the mouse was placed on a wire grid at pulled by its tail”
Evaluated	weekly	“Mice were evaluated weekly by neurological score, grip strength, hangwire test, rotarod test”
Evaluated	once a week	“evaluated by neurological score and grip strength once a week”
Evaluated	SOD1 males (n = 9) and females (n = 5) day 140	“SOD1 males (n = 9) and females (n = 5) were evaluated by neurological score, grip strength, hangwire test and y-maze test at day 140”
Performed	between 9 am and 2 pm	“All clinical behavioral tests were performed between 9 am and 2 pm”
Placed in the room	one hour before test sessions	“mice were placed in the room one hour before test sessions to allow time for acclimatization”
Cleaned with	70% ethanol between each animal	“Test equipment was cleaned with 70% ethanol between each animal”
Apparatus
Grip strength meter	Bioseb	“Grip strength was evaluated using Grip strength meter (Bioseb, France)”
Wire grid		“the mouse was placed on a wire grid”
Methods cited to other work
Normalizing the mean grip strength to weight	^24	“The mean grip strength was normalized to weight as previously described^24”

Rotarod test

Compare with 9 other papers

Setting	Value	Paper’s words
Rod speed	4 to 40 RPM	“an acceleration from 4 to 40 RPM over 5 min”
Trial duration	Unclear 5 min 5 min is printed as the time over which the rod accelerates. The passage does not say that a trial ends at 5 min or give the longest length of a trial.	“an acceleration from 4 to 40 RPM over 5 min”
Number of trials	three times	“Each mouse was tested three times per test session”
Other settings in the paper
Acceleration	over 5 min	“an acceleration from 4 to 40 RPM over 5 min”
Acclimatized to the rotarod	Before the first test session three consecutive days	“Mice were acclimatized to the rotarod over three consecutive days before the first test session”
Evaluated	weekly	“Mice were evaluated weekly by neurological score, grip strength, hangwire test, rotarod test”
Performed	between 9 am and 2 pm	“All clinical behavioral tests were performed between 9 am and 2 pm”
Placed in the room	one hour before test sessions	“mice were placed in the room one hour before test sessions to allow time for acclimatization”
Cleaned with	70% ethanol between each animal	“Test equipment was cleaned with 70% ethanol between each animal”
Apparatus
Rotamex-5 RotaRod	Columbus Instruments	“Rotarod test (Rotamex-5 RotaRod, Columbus Instruments, Columbus, Ohio, USA)”

Y-maze test

Compare with 7 other papers

Setting	Value	Paper’s words
Session duration	5 min	“The mice were placed in the y-maze for 5 min”
Arm size	Cited to other work No arm dimensions are printed. The passage sends the reader to Maze Engineers for how the maze was constructed.	“The y-maze test was constructed according to Maze Engineers (USA)”
Other settings in the paper
Arms	three	“to freely explore the three arms”
Blinded raters	four	“the number of entries and triplets were noted by four blinded raters”
Evaluated	CPT1A antagonist studies day 70, day 100 and day 130	“at day 70, day 100 and day 130 mice were evaluated by cylinder, and y-maze test”
Evaluated	SOD1Wt/Cpt1a and SOD1Cpt1a/Cpt1a studies day 70, 100, and 130	“mice were evaluated at day 70, 100, and 130 using cylinder (n = 7–11), and y-maze test (n = 6–9)”
Evaluated	SOD1 G93A High-fat diet study day 70, and day 130	“mice were evaluated at day 70, and day 130 using cylinder, and y-maze test”
Evaluated	SOD1 G93A corticosterone study day 70, and day 100	“mice were evaluated at day 70, and day 100 using cylinder and y-maze test”
Evaluated	SOD1 males (n = 9) and females (n = 5) day 140	“SOD1 males (n = 9) and females (n = 5) were evaluated by neurological score, grip strength, hangwire test and y-maze test at day 140”
Performed	between 9 am and 2 pm	“All clinical behavioral tests were performed between 9 am and 2 pm”
Placed in the room	one hour before test sessions	“mice were placed in the room one hour before test sessions to allow time for acclimatization”
Cleaned with	70% ethanol between each animal	“Test equipment was cleaned with 70% ethanol between each animal”
Apparatus
Y-maze	Maze Engineers	“The y-maze test was constructed according to Maze Engineers (USA)”
Video		“The y-maze test was recorded by video”
Methods cited to other work
How entries and triplets were scored	^99	“the number of entries and triplets were noted by four blinded raters as previously described^99”
Calculating the spontaneous alternation percentage	^99	“The mean spontaneous alternation percentage was calculated as previously described^99”
Construction of the maze	Maze Engineers (USA)

Downregulating carnitine palmitoyl transferase 1 affects disease progression in the SOD1 G93A mouse model of ALS

Trabjerg MS, Andersen DC, Huntjens P, et al. Communications biology 2021;4(1):509.

The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made

Grip strength test

Compare with 6 other papers

Setting	Value	Paper’s words
Number of trials	four trials	“Each mouse received four trials at each session”
Other settings in the paper
Maximum tension	grams	“The maximum tension was measured in grams”
Pulled by	its tail	“the mouse was placed on a wire grid at pulled by its tail”
Evaluated	weekly	“Mice were evaluated weekly by neurological score, grip strength, hangwire test, rotarod test”
Evaluated	once a week	“evaluated by neurological score and grip strength once a week”
Evaluated	SOD1 males (n = 9) and females (n = 5) day 140	“SOD1 males (n = 9) and females (n = 5) were evaluated by neurological score, grip strength, hangwire test and y-maze test at day 140”
Performed	between 9 am and 2 pm	“All clinical behavioral tests were performed between 9 am and 2 pm”
Placed in the room	one hour before test sessions	“mice were placed in the room one hour before test sessions to allow time for acclimatization”
Cleaned with	70% ethanol between each animal	“Test equipment was cleaned with 70% ethanol between each animal”
Apparatus
Grip strength meter	Bioseb	“Grip strength was evaluated using Grip strength meter (Bioseb, France)”
Wire grid		“the mouse was placed on a wire grid”
Methods cited to other work
Normalizing the mean grip strength to weight	^24	“The mean grip strength was normalized to weight as previously described^24”

Rotarod test

Compare with 9 other papers

Setting	Value	Paper’s words
Rod speed	4 to 40 RPM	“an acceleration from 4 to 40 RPM over 5 min”
Trial duration	Unclear 5 min 5 min is printed as the time over which the rod accelerates. The passage does not say that a trial ends at 5 min or give the longest length of a trial.	“an acceleration from 4 to 40 RPM over 5 min”
Number of trials	three times	“Each mouse was tested three times per test session”
Other settings in the paper
Acceleration	over 5 min	“an acceleration from 4 to 40 RPM over 5 min”
Acclimatized to the rotarod	Before the first test session three consecutive days	“Mice were acclimatized to the rotarod over three consecutive days before the first test session”
Evaluated	weekly	“Mice were evaluated weekly by neurological score, grip strength, hangwire test, rotarod test”
Performed	between 9 am and 2 pm	“All clinical behavioral tests were performed between 9 am and 2 pm”
Placed in the room	one hour before test sessions	“mice were placed in the room one hour before test sessions to allow time for acclimatization”
Cleaned with	70% ethanol between each animal	“Test equipment was cleaned with 70% ethanol between each animal”
Apparatus
Rotamex-5 RotaRod	Columbus Instruments	“Rotarod test (Rotamex-5 RotaRod, Columbus Instruments, Columbus, Ohio, USA)”

Y-maze test

Compare with 7 other papers

Setting	Value	Paper’s words
Session duration	5 min	“The mice were placed in the y-maze for 5 min”
Arm size	Cited to other work No arm dimensions are printed. The passage sends the reader to Maze Engineers for how the maze was constructed.	“The y-maze test was constructed according to Maze Engineers (USA)”
Other settings in the paper
Arms	three	“to freely explore the three arms”
Blinded raters	four	“the number of entries and triplets were noted by four blinded raters”
Evaluated	CPT1A antagonist studies day 70, day 100 and day 130	“at day 70, day 100 and day 130 mice were evaluated by cylinder, and y-maze test”
Evaluated	SOD1Wt/Cpt1a and SOD1Cpt1a/Cpt1a studies day 70, 100, and 130	“mice were evaluated at day 70, 100, and 130 using cylinder (n = 7–11), and y-maze test (n = 6–9)”
Evaluated	SOD1 G93A High-fat diet study day 70, and day 130	“mice were evaluated at day 70, and day 130 using cylinder, and y-maze test”
Evaluated	SOD1 G93A corticosterone study day 70, and day 100	“mice were evaluated at day 70, and day 100 using cylinder and y-maze test”
Evaluated	SOD1 males (n = 9) and females (n = 5) day 140	“SOD1 males (n = 9) and females (n = 5) were evaluated by neurological score, grip strength, hangwire test and y-maze test at day 140”
Performed	between 9 am and 2 pm	“All clinical behavioral tests were performed between 9 am and 2 pm”
Placed in the room	one hour before test sessions	“mice were placed in the room one hour before test sessions to allow time for acclimatization”
Cleaned with	70% ethanol between each animal	“Test equipment was cleaned with 70% ethanol between each animal”
Apparatus
Y-maze	Maze Engineers	“The y-maze test was constructed according to Maze Engineers (USA)”
Video		“The y-maze test was recorded by video”
Methods cited to other work
How entries and triplets were scored	^99	“the number of entries and triplets were noted by four blinded raters as previously described^99”
Calculating the spontaneous alternation percentage	^99	“The mean spontaneous alternation percentage was calculated as previously described^99”
Construction of the maze	Maze Engineers (USA)