ReplicateScience
TestsPapersMethodologyAboutOffline
Submit a paper
ReplicateScience

Records of how behavioral tests were run, taken from open-access papers. Built by ConductScience.

Browse

  • Tests
  • Papers
  • Scientists
  • Institutions

About

  • Methodology
  • About
  • Submit a paper
  • Offline edition
  • ConductScience

Legal

  • Privacy
  • Terms

© 2026 ConductScience. All rights reserved.

Tests/Balance beam test

Balance beam test

How 5 papers set up this test.

AI read these papers and code checked every quote. No person has reviewed the records yet. How records are made

Beam size

Length, and width or diameter, of the beam

PaperValuePaper’s words
Wen et al. 2024Mice
  • 80 cm-long

  • Beam diameters

    12 mm

  • Beam diameters

    6 mm

“An 80 cm-long beam is elevated 50 cm from the floor”
“Two different beam diameters were used: 12 mm and 6 mm”
Drapeau et al. 2018Mice
  • 100 cm long

  • Three different beams

    1 inch

  • Three different beams

    ½ inch

  • Three different beams

    ¼ inch

“an elevated horizontal wood beam (100 cm long, 1 m above bedding)”
“three different beams: 1 inch, ½ inch and ¼ inch diameter”
Xin et al. 2017Mice
  • 100-cm long, 10-mm thick

“a 100-cm long, 10-mm thick beam”
Florez-Paz et al. 2016Mice
  • 1 m long, 2 cm wide

“Balance was tested on a 1 m long, 2 cm wide beam”
Trabjerg et al. 2023Mice
  • The first

    16 mm at the broadest and narrowing down to 12 mm

  • The second

    6 mm

  • Day 42

    Unclear

    0.6 cm

    One passage does not say what 0.6 cm measures. It matches the 6 mm width printed in another passage, so it is likely the same beam.

“the first being with a width of 16 mm at the broadest and narrowing down to 12 mm”
“the second with a width of 6 mm”
“transverse the 0.6 cm beam”
“compared to Wt+R+V at day 42”

Beam height

Height of the beam above the floor or padding

PaperValuePaper’s words
Wen et al. 2024Mice
  • 50 cm

“An 80 cm-long beam is elevated 50 cm from the floor”
Drapeau et al. 2018Mice
  • 1 m

“an elevated horizontal wood beam (100 cm long, 1 m above bedding)”
Xin et al. 2017Mice
  • 70 cm

“beam was placed at a height of 70 cm”
Florez-Paz et al. 2016Mice
  • 50 cm

“beam suspended on two poles 50 cm above a table top”
Trabjerg et al. 2023Mice
  • Cited to other work

    No height is printed; the passage sends the reader to the cited protocol for the whole test.

“The test was conducted according to the protocol^105”

Number of trials

How many trials an animal gets in one session or day

PaperValuePaper’s words
Wen et al. 2024Mice
  • Trained

    three trials per beam width each day

  • Tested

    three trials per beam width

“Each animal was trained for two consecutive days to cross the beam three trials per beam width each day”
“Each animal was tested for three trials per beam width with 5–10 min of rest between trials”
Drapeau et al. 2018Mice
  • Per beam

    four consecutive trials

“scored on four consecutive trials per beam”
Florez-Paz et al. 2016Mice
  • Unclear

    at least three times

    One passage covers all tests at once. It gives a lower bound on repetitions per animal but does not say whether these fell within one session or day or across sessions.

“each of which was repeated at least three times in each animal”
Trabjerg et al. 2023Mice
  • Cited to other work

    No trial count is printed; the passage sends the reader to the cited protocol for the whole test.

“The test was conducted according to the protocol^105”

Not in the Methods of Xin et al. 2017.

Other settings the papers report (45)

Settings outside the list above, named as each paper names them.

PaperSetting and valuePaper’s words
Xin et al. 2017Mice

Acclimate to the goal box, training

10 min

“During training, the mouse was allowed to acclimate to the goal box for 10 min.”
Trabjerg et al. 2023Mice

All tests were performed

between 10:00 and 16:00 in the lights-on cycle

“All tests were performed between 10:00 and 16:00 in the lights-on cycle”
Drapeau et al. 2018Mice

Allowed to stay inside

1 min

“allowed to stay inside for 1 min”
Trabjerg et al. 2023Mice

Animals were excluded from behavioral tests if

the video camera stopped recording before the test was done

“Animals were excluded from behavioral tests if the video camera stopped recording before the test was done”
Florez-Paz et al. 2016Mice

Aversive stimulus

a lamp above the start point

“a lamp above the start point served as an aversive stimulus”
Drapeau et al. 2018Mice

Beam

wood

“an elevated horizontal wood beam”
Trabjerg et al. 2023Mice

Beam test, chronic rotenone mouse models

day 42

“beam test at day 42”
Trabjerg et al. 2023Mice

Beams

two

“Mice had to traverse two different beams”
Drapeau et al. 2018Mice

Behavioral experiments were conducted

between 9 A.M. and 5 P.M.

“Behavioral experiments were conducted between 9 A.M. and 5 P.M.”
Drapeau et al. 2018Mice

Between each beam

20–30 min

“20–30 min between each beam”
Florez-Paz et al. 2016Mice

Box at the finish point

Food

“Food was placed in a box at the finish point to attract the mouse”
Drapeau et al. 2018Mice

Bright light, near one end

bright light

“Subjects were placed near one end in bright light”
Drapeau et al. 2018Mice

Darkness, far end with the dark box

darkness

“the far end with the dark box was placed in darkness”
Trabjerg et al. 2023Mice

Data were excluded if

the mice turned around continuously or walked in the wrong direction

“Data were excluded if the mice turned around continuously or walked in the wrong direction”
Drapeau et al. 2018Mice

Did not reach the box after

2 min

“Mice that did not reach the box after 2 min”
Drapeau et al. 2018Mice

Different beams

three

“three different beams”
Wen et al. 2024Mice

Divided into

two batches

“animals were randomly divided into two batches and tested separately for balance beam, DigiGait, and Barnes maze tests”
Trabjerg et al. 2023Mice

Experimenters

blinded to treatment groups

“The experimenters were blinded to treatment groups for all behavioral studies”
Trabjerg et al. 2023Mice

Exposed to behavioral tests

in a randomized order

“The mice were exposed to behavioral tests in a randomized order”
Wen et al. 2024Mice

Fell from the beam

placed back on the end of the beam to restart crossing

“If an animal fell from the beam, it was placed back on the end of the beam to restart crossing”
Wen et al. 2024Mice

First tested on, During the test day

the 12 mm diameter beam and then the 6 mm diameter beam

“During the test day, mice were first tested on the 12 mm diameter beam and then the 6 mm diameter beam”
Trabjerg et al. 2023Mice

Full cleaning protocol, Between every test

70% ethanol

“Between every test, a full cleaning protocol by 70% ethanol was applied to the surfaces of the equipment”
Wen et al. 2024Mice

Invalid

A trial with stall

“A trial with stall was invalid and cannot be scored for the latency to cross”
Wen et al. 2024Mice

Invalid

falls or stalls for more than 5 s and will not proceed without prompting

“A trial was also invalid if the mouse falls or stalls for more than 5 s and will not proceed without prompting”
Trabjerg et al. 2023Mice

Mice were acclimatized to the laboratory

30 min before tests began

“mice were acclimatized to the laboratory 30 min before tests began”
Drapeau et al. 2018Mice

Mice were brought to the front room of the testing area

at least half an hour

“Mice were brought to the front room of the testing area at least half an hour before the start of experiments”
Wen et al. 2024Mice

Order of tests, batch 1

Day 29–31

“Day 29–31, balance beam for batch 1”
Wen et al. 2024Mice

Order of tests, batch 2

Day 36–38

“Day 36–38, balance beam for batch 2”
Wen et al. 2024Mice

Pause

less than 5 s

“A pause is defined as the mouse pauses for less than 5 s and then proceeds without prompting”
Wen et al. 2024Mice

Prompt, During training day 1

its tail was gently prompt to encourage movement

“During training day 1, if an animal stopped crossing the beam, its tail was gently prompt to encourage movement”
Wen et al. 2024Mice

Prompting, training day 2 and on the testing day

No prompting

“No prompting was performed during training day 2 and on the testing day”
Xin et al. 2017Mice

Repeated, Thereafter

until the mice could no longer complete the tests

“Thereafter, the tests were repeated once a week until the mice could no longer complete the tests.”
Wen et al. 2024Mice

Rest between trials, tested

5–10 min

“Each animal was tested for three trials per beam width with 5–10 min of rest between trials”
Drapeau et al. 2018Mice

Rest between trials on the same beam

1 min

“with 1 min of rest between trials on the same beam”
Wen et al. 2024Mice

Rest in between tests

at least a 1-day

“Animals were given at least a 1-day rest in between tests”
Wen et al. 2024Mice

Safe zone

containing clean bedding and food

“A safe zone is attached to one end of the beam, containing clean bedding and food”
Wen et al. 2024Mice

Stall

more than 5 s

“A stall is defined as the mouse pauses for more than 5 s and then proceeds without prompting”
Xin et al. 2017Mice

Tested

on the fourth day

“The mice were trained for three consecutive days and were tested on the fourth day.”
Wen et al. 2024Mice

Tested for

1 day

“and then tested for 1 day”
Xin et al. 2017Mice

Tests were repeated, Thereafter

once a week

“Thereafter, the tests were repeated once a week until the mice could no longer complete the tests.”
Xin et al. 2017Mice

Trained

three consecutive days

“The mice were trained for three consecutive days and were tested on the fourth day.”
Wen et al. 2024Mice

Trained for

two consecutive days

“Each animal was trained for two consecutive days”
Trabjerg et al. 2023Mice

Trained until, Prior to the challenging transversal beam test

they were able to spontaneously cross the beam

“Prior to the challenging transversal beam test, the mice were trained until they were able to spontaneously cross the beam”
Trabjerg et al. 2023Mice

Video material was rated by, depending on the experiment

three to four blinded individuals

“Video material was rated by three to four blinded individuals, depending on the experiment”
Trabjerg et al. 2023Mice

Were defined by the presence of, At day 32

a minimum two of the following factors

“At day 32, animals, who did not show signs of disease, were defined by the presence of a minimum two of the following factors: lower grip strength, lower latency to fall on the rotarod, longer time to transverse the beam, pole or lower number of rears in the cylinder test”

Apparatus

What each paper names for this test. Vendors and models appear as the paper prints them.

PaperApparatusPaper’s words
Wen et al. 2024

Balance beam

Conduct Science MazeEngineers

“Balance beam test (Conduct Science MazeEngineers, Skokie, IL)”
Wen et al. 2024

Front camera

“Mice behavior were recorded using a front camera and an overhead camera”
Wen et al. 2024

Overhead camera

“Mice behavior were recorded using a front camera and an overhead camera”
Trabjerg et al. 2023

Beams

“Mice had to traverse two different beams”
Trabjerg et al. 2023

Video camera

“A video camera was used to record each trial”
Drapeau et al. 2018

Wood beam

“an elevated horizontal wood beam (100 cm long, 1 m above bedding)”
Drapeau et al. 2018

Dark box

“to a safe dark box”
Xin et al. 2017

Balance beam

“For the balance beam [33, 34], a 100-cm long, 10-mm thick beam was placed at a height of 70 cm with an enclosed goal box at one end.”
Florez-Paz et al. 2016

Balance beam

“Balance was tested on a 1 m long, 2 cm wide beam suspended on two poles 50 cm above a table top. Food was placed in a box at the finish point to attract the mouse and a lamp above the start point served as an aversive stimulus. A video camera on a tripod was used to record the time required to cross the beam.”