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Papers/Xin et al. 2017

Manganese transporter Slc39a14 deficiency revealed its key role in maintaining manganese homeostasis in mice

Xin Y, Gao H, Wang J, et al. Cell discovery 2017;3:17025.

The test records were read three times by AI and checked by code. No person has reviewed them yet. How records are made

Tests in this paper

  • Balance beam test
  • Gait analysis
  • Rotarod test

Balance beam test

Compare with 4 other papers
SettingValuePaper’s words
Beam size
  • 100-cm long, 10-mm thick

“a 100-cm long, 10-mm thick beam”
Beam height
  • 70 cm

“beam was placed at a height of 70 cm”
Other settings in the paper
Acclimate to the goal box

Training

10 min

“During training, the mouse was allowed to acclimate to the goal box for 10 min.”
Trained

three consecutive days

“The mice were trained for three consecutive days and were tested on the fourth day.”
Tested

on the fourth day

“The mice were trained for three consecutive days and were tested on the fourth day.”
Tests were repeated

Thereafter

once a week

“Thereafter, the tests were repeated once a week until the mice could no longer complete the tests.”
Repeated

Thereafter

until the mice could no longer complete the tests

“Thereafter, the tests were repeated once a week until the mice could no longer complete the tests.”
Apparatus
Balance beam
“For the balance beam [33, 34], a 100-cm long, 10-mm thick beam was placed at a height of 70 cm with an enclosed goal box at one end.”

Gait analysis

Compare with 8 other papers
SettingValuePaper’s words
Run duration criterion
  • ⩽10 s

“A successful trial was defined as the mouse taking ⩽10 s to cross the field of vision of a video camera”
Number of runs
  • Each training day

    three trials

  • The test day

    two trials

“Each mouse successfully completed three trials on each training day”
“Each mouse successfully completed three trials on each training day and two trials on the test day.”
Not in the MethodsWalkway size
Other settings in the paper
Allowed to acclimate

10 min

“the mouse was placed in an enclosed box at the end of the walkway and allowed to acclimate for 10 min”
Light on the walkway

green

“with a green light on the walkway and a red ceiling light to illuminate the mouse”
Ceiling light

red

“with a green light on the walkway and a red ceiling light to illuminate the mouse”
Lights

turned off

“after which the lights were turned off”
Trained

three consecutive days

“The mice were trained for three consecutive days and were tested on the fourth day.”
Tested

the fourth day

“The mice were trained for three consecutive days and were tested on the fourth day.”
Tests were repeated

once a week

“Thereafter, the tests were repeated once a week until the mice could no longer complete the tests.”
Apparatus
CatWalk XT system

Noldus Information Technology

“we used the CatWalk XT system [37–39] (Noldus Information Technology, Wageningen, The Netherlands)”
CatWalk XT software
“which was analyzed by CatWalk XT software”

Rotarod test

Compare with 9 other papers
SettingValuePaper’s words
Rod speed
  • Training

    10, 12 and 14 r.p.m.

  • Testing

    4 to 40 r.p.m.

“three 5-min training trials with the speed set to 10, 12 and 14 r.p.m.”
“During testing, the rotarod speed was increased from 4 to 40 r.p.m. over a 5-min period”
Trial duration
  • Training

    5-min

  • Testing

    Unclear

    5-min

    The 5-min value is the period over which the rod accelerated. The passage does not say that the test trial ended or was cut off at 5 min.

“three 5-min training trials”
“the rotarod speed was increased from 4 to 40 r.p.m. over a 5-min period”
Number of trials
  • Training

    Unclear

    three

    Three training trials are given alongside three consecutive training days. The passage does not say whether three trials were given each day or three in total (one per day at 10, 12 and 14 r.p.m.).

  • Testing

    Not stated

    The number of trials on the test day is not given.

“with three 5-min training trials with the speed set to 10, 12 and 14 r.p.m.”
“trained on the rotarod for three consecutive days”
Other settings in the paper
Trained on the rotarod

Training

three consecutive days

“trained on the rotarod for three consecutive days”
Period over which the rotarod speed was increased

Testing

5-min

“the rotarod speed was increased from 4 to 40 r.p.m. over a 5-min period”
Were tested

on the fourth day

“The mice were trained for three consecutive days and were tested on the fourth day.”
The tests were repeated

once a week

“Thereafter, the tests were repeated once a week until the mice could no longer complete the tests.”
Apparatus
Accelerating rotarod

Softmaze, XR1514

“The accelerating rotarod [35, 36] (Softmaze-XR1514, Shanghai, China) consisted of a felt-covered steel cylinder fitted to a rotating pump with a variable speed setting.”

Tests in this paper

  • Balance beam test
  • Gait analysis
  • Rotarod test

The paper

Species: mice

“Slc39a14^fl/+ mice were backcrossed to C57BL6/J mice”
Read the paper (doi:10.1038/celldisc.2017.25)Full text on PubMed Central (PMC5519003)

Quotes are shown under the paper’s CC BY license.

How far this was checked

The test records were read three times by AI and checked by code. No person has reviewed them yet.

How records are made