Gait analysis

How 9 papers set up this test.

AI read these papers and code checked every quote. No person has reviewed the records yet. How records are made

Walkway size

Dimensions of the gait walkway or runway

Paper	Value	Paper’s words
Drapeau et al. 2018Mice	152 cm long × 10 cm wide	“an elevated runway (dimensions: 152 cm long × 10 cm wide)”
Florez-Paz et al. 2016Mice	85 cm long and 8.5 cm wide	“the mice were placed in a glass chamber (85 cm long and 8.5 cm wide) and they were allowed to move freely across the walkway”
Ahire et al. 2023Mice	Cited to other work No walkway dimensions are given; the CatWalk procedure is described only by citing a previously reported protocol.	“using our previously reported protocol (Ungvari et al., 2017)”
Fang et al. 2018Mice	Cited to other work The glass platform is named but no dimensions are given. The Catwalk acquisition method is referred to ref. 22.	“captured by the Catwalk^® gait analysis system (Noldus Inc.) as described^22”

Not in the Methods of Tsuji et al. 2026, Johnston et al. 2025, Wen et al. 2024, Xin et al. 2017, Huang et al. 2016.

Run duration criterion

The time limits a run must fall within to count

Paper	Value	Paper’s words
Xin et al. 2017Mice	⩽10 s	“A successful trial was defined as the mouse taking ⩽10 s to cross the field of vision of a video camera”
Wen et al. 2024Mice	Unclear within 5 s The 5 s is a time window for 10 consecutive strides in a treadmill video. It limits which videos were analyzed, but the passage does not call it a limit on run duration.	“Videos with at least 10 consecutive strides within 5 s were analyzed using the DigiGait software”
Ahire et al. 2023Mice	Cited to other work No run duration criterion is given; the CatWalk procedure is described only by citing a previously reported protocol.	“using our previously reported protocol (Ungvari et al., 2017)”
Fang et al. 2018Mice	Cited to other work No run duration limits are given. The acquisition method is referred to ref. 22.	“captured by the Catwalk^® gait analysis system (Noldus Inc.) as described^22”

Not in the Methods of Tsuji et al. 2026, Johnston et al. 2025, Drapeau et al. 2018, Florez-Paz et al. 2016, Huang et al. 2016.

Number of runs

How many runs are recorded or required for each animal

Paper	Value	Paper’s words
Drapeau et al. 2018Mice	two consecutive runs	“to record paw prints on two consecutive runs”
Xin et al. 2017Mice	Each training day three trials The test day two trials	“Each mouse successfully completed three trials on each training day” “Each mouse successfully completed three trials on each training day and two trials on the test day.”
Florez-Paz et al. 2016Mice	at least three times	“scored all the tests, each of which was repeated at least three times in each animal” “the values from the individual tracks were averaged”
Ahire et al. 2023Mice	Cited to other work No number of runs is given; the CatWalk procedure is described only by citing a previously reported protocol.	“using our previously reported protocol (Ungvari et al., 2017)”
Fang et al. 2018Mice	Cited to other work The number of runs per mouse is not given. The acquisition method is referred to ref. 22.	“captured by the Catwalk^® gait analysis system (Noldus Inc.) as described^22”

Not in the Methods of Tsuji et al. 2026, Johnston et al. 2025, Wen et al. 2024, Huang et al. 2016.

Camera frame rate

Frames per second of the camera that records the runs

Paper	Value	Paper’s words
Ahire et al. 2023Mice	Cited to other work No frame rate is given; the whole CatWalk protocol is referred to prior work.	“using our previously reported protocol (Ungvari et al., 2017)”
Fang et al. 2018Mice	Cited to other work A camera underneath the platform is mentioned, but no frame rate is given. The acquisition method is referred to ref. 22.	“captured by the Catwalk^® gait analysis system (Noldus Inc.) as described^22”

Not in the Methods of Tsuji et al. 2026, Johnston et al. 2025, Wen et al. 2024, Drapeau et al. 2018, Xin et al. 2017, Florez-Paz et al. 2016, Huang et al. 2016.

Other settings the papers report (33)

Settings outside the list above, named as each paper names them.

Paper	Setting and value	Paper’s words
Wen et al. 2024Mice	Acclimated in the treadmill compartment 1 min	“Animal was acclimated in the treadmill compartment for 1 min”
Xin et al. 2017Mice	Allowed to acclimate 10 min	“the mouse was placed in an enclosed box at the end of the walkway and allowed to acclimate for 10 min”
Drapeau et al. 2018Mice	Analysis of 50 cm	“for analysis of 50 cm”
Drapeau et al. 2018Mice	Behavioral experiments were conducted between 9 A.M. and 5 P.M.	“Behavioral experiments were conducted between 9 A.M. and 5 P.M.”
Xin et al. 2017Mice	Ceiling light red	“with a green light on the walkway and a red ceiling light to illuminate the mouse”
Wen et al. 2024Mice	Consecutive strides at least 10	“Videos with at least 10 consecutive strides within 5 s were analyzed using the DigiGait software”
Wen et al. 2024Mice	Divided into two batches	“animals were randomly divided into two batches and tested separately for balance beam, DigiGait, and Barnes maze tests”
Wen et al. 2024Mice	Gradually increased to the testing speed over several seconds	“gradually increased to the testing speed over several seconds”
Wen et al. 2024Mice	High-speed video camera positioned below the transparent belt	“a high-speed video camera positioned below the transparent belt”
Xin et al. 2017Mice	Light on the walkway green	“with a green light on the walkway and a red ceiling light to illuminate the mouse”
Xin et al. 2017Mice	Lights turned off	“after which the lights were turned off”
Drapeau et al. 2018Mice	Lined with white paper	“lined with white paper”
Wen et al. 2024Mice	Low speed 5 cm/s	“treadmill was turned on at a low speed (5 cm/s)”
Johnston et al. 2025Mice	Measurements were taken from the center of the right hind paw to the same hind paw center a stride later	“Measurements were taken from the center of the right hind paw to the same hind paw center a stride later”
Drapeau et al. 2018Mice	Mice were brought to the front room of the testing area at least half an hour	“Mice were brought to the front room of the testing area at least half an hour before the start of experiments”
Drapeau et al. 2018Mice	Nontoxic paint different colors for hind and front paws	“coated in nontoxic paint (different colors for hind and front paws)”
Wen et al. 2024Mice	Order of tests, batch 1 Day 33	“Day 33, DigiGait for batch 1”
Wen et al. 2024Mice	Order of tests, batch 2 Day 40	“Day 40, DigiGait for batch 2”
Johnston et al. 2025Mice	Paws dipped in water-soluble paint	“had paws dipped in water-soluble paint”
Johnston et al. 2025Mice	Placed on a large sheet of white paper	“were placed on a large sheet of white paper”
Tsuji et al. 2026Mice	Preflight metrics, first down-selection suboptimal or poor	“The first down-selection process excluded mice that exhibited health concerns, suboptimal or poor preflight metrics [<1 fecal pellet, dual-energy x-ray absorptiometry (DXA), gait analysis using DigiGait (Mouse Specifics Inc., Seaford, DE, USA)]”
Wen et al. 2024Mice	Rest in between tests at least a 1-day	“Animals were given at least a 1-day rest in between tests”
Fang et al. 2018Mice	Room dark	“mice were placed on a glass platform located in a dark room”
Wen et al. 2024Mice	Speeds 10 cm/s, 20 cm/s, and 30 cm/s	“Each mouse was tested on three speeds: 10 cm/s, 20 cm/s, and 30 cm/s”
Xin et al. 2017Mice	Tested the fourth day	“The mice were trained for three consecutive days and were tested on the fourth day.”
Xin et al. 2017Mice	Tests were repeated once a week	“Thereafter, the tests were repeated once a week until the mice could no longer complete the tests.”
Drapeau et al. 2018Mice	The record displaying the clearest prints and most consistent gait	“The record displaying the clearest prints and most consistent gait”
Huang et al. 2016Rats	The test was performed 1, 2, 3, and 6 weeks after injury	“The test was performed 1, 2, 3, and 6 weeks after injury.”
Xin et al. 2017Mice	Trained three consecutive days	“The mice were trained for three consecutive days and were tested on the fourth day.”
Drapeau et al. 2018Mice	Training runs three	“After three training runs”
Fang et al. 2018Mice	Transferred to the Behavior Study Suite ... prior to gait acquisitions two days	“transferred to the Behavior Study Suite at Robarts Research Institute at Western University two days prior to gait acquisitions”
Wen et al. 2024Mice	Unable to walk at the target speed removed from the study	“Animals that were unable to walk at the target speed were removed from the study”
Huang et al. 2016Rats	Wide-angle lens 8.5-mm	“equipped with an 8.5-mm wide-angle lens”

Apparatus

What each paper names for this test. Vendors and models appear as the paper prints them.

Paper	Apparatus	Paper’s words
Tsuji et al. 2026	DigiGait Mouse Specifics Inc.	“gait analysis using DigiGait (Mouse Specifics Inc., Seaford, DE, USA)”
Johnston et al. 2025	Water-soluble paint	“had paws dipped in water-soluble paint”
Johnston et al. 2025	Large sheet of white paper	“were placed on a large sheet of white paper to measure gait/stride length”
Wen et al. 2024	DigiGait Imaging System Mouse Specifics	“the DigiGait Imaging System (Mouse Specifics, Framingham, MA)”
Wen et al. 2024	DigiGait treadmill	“Videos of the mice walked on the DigiGait treadmill”
Wen et al. 2024	High-speed video camera	“a high-speed video camera positioned below the transparent belt”
Wen et al. 2024	DigiGait software	“were analyzed using the DigiGait software”
Ahire et al. 2023	CatWalk Noldus Information Technology Inc.	“we tested the experimental groups of mice using an automated computer assisted method (CatWalk; Noldus Information Technology Inc.)”
Drapeau et al. 2018	Elevated runway	“an elevated runway (dimensions: 152 cm long × 10 cm wide)”
Drapeau et al. 2018	White paper	“lined with white paper”
Drapeau et al. 2018	Nontoxic paint	“paws were coated in nontoxic paint”
Fang et al. 2018	Catwalk^® gait analysis system Noldus Inc.	“Gait patterns and relative parameters were captured by the Catwalk^® gait analysis system (Noldus Inc.) as described^22.”
Fang et al. 2018	Catwalk^® software	“Gait was captured and parameters was obtained from the Catwalk^® software for statistical analysis.”
Xin et al. 2017	CatWalk XT system Noldus Information Technology	“we used the CatWalk XT system [37–39] (Noldus Information Technology, Wageningen, The Netherlands)”
Xin et al. 2017	CatWalk XT software	“which was analyzed by CatWalk XT software”
Florez-Paz et al. 2016	CatWalk system 7.1 Noldus Information Technology	“The animal’s gait was tested with the CatWalk system 7.1 (Noldus Information Technology), where the mice were placed in a glass chamber (85 cm long and 8.5 cm wide) and they were allowed to move freely across the walkway.”
Florez-Paz et al. 2016	CatWalk software	“allowing the paw prints to be captured by a video camera and analyzed with the CatWalk software”
Huang et al. 2016	CatWalk system Noldus Inc.	“Motor function was determined by stand time and footprint intensity using the CatWalk system (Noldus Inc., Wageningen, Netherlands)”
Huang et al. 2016	High-speed video camera Gevicam, GP-3360	“a GP-3360 high-speed video camera (Gevicam, Milpitas, CA, USA)”
Huang et al. 2016	Wide-angle lens Fujicon Corp.	“equipped with an 8.5-mm wide-angle lens (Fujicon Corp., Shenzhen, China)”