Tsuji R, Fujita R, Hayashi T, et al. Science advances 2026;12(11):eaed2258.
The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made
| Setting | Value | Paper’s words |
|---|---|---|
| Not in the Methods | Walkway size, run duration criterion, number of runs | |
| Other settings in the paper | ||
| Preflight metrics | First down-selection suboptimal or poor | “The first down-selection process excluded mice that exhibited health concerns, suboptimal or poor preflight metrics [<1 fecal pellet, dual-energy x-ray absorptiometry (DXA), gait analysis using DigiGait (Mouse Specifics Inc., Seaford, DE, USA)]” |
| Apparatus | ||
| DigiGait | Mouse Specifics Inc. | “gait analysis using DigiGait (Mouse Specifics Inc., Seaford, DE, USA)” |
| Setting | Value | Paper’s words |
|---|---|---|
| Number of trials |
| “Three trials were performed for both tests” |
| Other settings in the paper | ||
| Capacity | 50-N | “a 50-N capacity grip strength meter” |
| Tested | before and immediately following live animal return | “Mouse grip strength was tested before and immediately following live animal return” |
| Delay | GC animals 2-day delay | “We also measured grip strength in GC animals set on a 2-day delay” |
| Blinded | blinded to the experimental conditions during flight | “The person performing the grip strength testing was blinded to the experimental conditions during flight” |
| Lowered over | Front paw testing a bar until they gripped it | “For front paw testing, the mice were lowered over a bar until they gripped it” |
| Gently pulled the base of the tail | Front paw testing to ensure that the animal remained horizontal | “The experimenter gently pulled the base of the tail to ensure that the animal remained horizontal” |
| Continued pulling | Front paw testing until the mouse released its grip | “then continued pulling until the mouse released its grip” |
| Gently restrained | Hind paw testing their hind paws were positioned near the bar until they gripped it | “For hind paw testing, the mice were gently restrained, and their hind paws were positioned near the bar until they gripped it” |
| Gently pulled the mouse | Hind paw testing until it released its grip | “The experimenter gently pulled the mouse until it released its grip” |
| Apparatus | ||
| Grip strength meter | Conduct Science | “using a 50-N capacity grip strength meter (Conduct Science, Skokie, IL, USA)” |