Growth hormone-deficient Ames dwarf mice resist sarcopenia and exhibit enhanced endurance running performance at 24 months

Johnston MJ, Rakoczy SG, Thompson LV, et al. GeroScience 2025;47(3):4827-4843.

The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made

Gait analysis

Setting	Value	Paper’s words
Not in the Methods	Walkway size, run duration criterion, number of runs
Other settings in the paper
Paws dipped in	water-soluble paint	“had paws dipped in water-soluble paint”
Placed on	a large sheet of white paper	“were placed on a large sheet of white paper”
Measurements were taken	from the center of the right hind paw to the same hind paw center a stride later	“Measurements were taken from the center of the right hind paw to the same hind paw center a stride later”
Apparatus
Water-soluble paint		“had paws dipped in water-soluble paint”
Large sheet of white paper		“were placed on a large sheet of white paper to measure gait/stride length”

Grip strength test

Compare with 6 other papers

Setting	Value	Paper’s words
Number of trials	5 grip strength trials	“animals were first weighed and then given a set of 5 grip strength trials”
Other settings in the paper
Limbs	all four limbs	“we modified this method to include all four limbs”
Maximum tensile force range	0 to 50 N	“a maximum tensile force range from 0 to 50 N”
Reading accuracy	0.1 g steps	“a reading accuracy of 0.1 g steps”
Error	≤ 0.3 g	“an error of ≤ 0.3 g”
Grid	steel grid	“both the fore and hind paws could grasp the steel grid”
Gently pulled away from the apparatus by the tail	until the grip was released	“gently pulled away from the apparatus by the tail until the grip was released”
Objective of the assessment	Peak force	“Peak force was the objective of the assessment”
Fitness testing	once per month	“All participants underwent fitness testing once per month over a period of 6 months”
Period	6 months	“All participants underwent fitness testing once per month over a period of 6 months”
Grip strength and rotarod tests were performed	on the same day	“Grip strength and rotarod tests were performed on the same day”
Apparatus
Maze Engineers Grip Strength Instrument		“Grip strength was recorded via Maze Engineers Grip Strength Instrument (Skokie, IL, USA)”
Methods cited to other work
The battery the fitness tests were derived from, the comprehensive functional assessment battery (CFAB)	[18]	“Fitness tests were derived from the comprehensive functional assessment battery (CFAB), a set of noninvasive tests used to assess and compare the physical function and motor task ability of rodents [18]”

Rotarod test

Compare with 9 other papers

Setting	Value	Paper’s words
Rod speed	Starting 4 revolutions/min Maximum speed 40 revolutions/min	“rotation starting at 4 revolutions/min” “until a maximum speed of 40 revolutions/min”
Trial duration	5 min	“Testing consisted of 5 min maximum latency”
Number of trials	3 trials	“On testing days, mice performed 3 trials of rotarod”
Other settings in the paper
Increasing steadily	7.2 revolutions/min^2	“increasing steadily at 7.2 revolutions/min^2”
Break	Between trials >10 min	“a break of>10 min between trials”
Rotating dowels	In half the lanes 50% smaller	“50% smaller rotating dowels in half the lanes”
Latency	automatically recorded via the motion sensor on the apparatus	“their latency was automatically recorded via the motion sensor on the apparatus”
Fitness testing	once per month	“All participants underwent fitness testing once per month over a period of 6 months”
Period	6 months	“All participants underwent fitness testing once per month over a period of 6 months”
Grip strength and rotarod tests were performed	on the same day	“Grip strength and rotarod tests were performed on the same day”
Apparatus
Maze Engineers Rotarod Instrument		“Rotarod testing was recorded utilizing Maze Engineers Rotarod Instrument (Skokie, IL, USA)”
Methods cited to other work
The battery the fitness tests were derived from, the comprehensive functional assessment battery (CFAB), and the use of rotarod as a performance and behavioral task	[18]	“Fitness tests were derived from the comprehensive functional assessment battery (CFAB), a set of noninvasive tests used to assess and compare the physical function and motor task ability of rodents [18]”

Growth hormone-deficient Ames dwarf mice resist sarcopenia and exhibit enhanced endurance running performance at 24 months

Johnston MJ, Rakoczy SG, Thompson LV, et al. GeroScience 2025;47(3):4827-4843.

The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made

Gait analysis

Compare with 8 other papers

Setting	Value	Paper’s words
Not in the Methods	Walkway size, run duration criterion, number of runs
Other settings in the paper
Paws dipped in	water-soluble paint	“had paws dipped in water-soluble paint”
Placed on	a large sheet of white paper	“were placed on a large sheet of white paper”
Measurements were taken	from the center of the right hind paw to the same hind paw center a stride later	“Measurements were taken from the center of the right hind paw to the same hind paw center a stride later”
Apparatus
Water-soluble paint		“had paws dipped in water-soluble paint”
Large sheet of white paper		“were placed on a large sheet of white paper to measure gait/stride length”

Grip strength test

Compare with 6 other papers

Setting	Value	Paper’s words
Number of trials	5 grip strength trials	“animals were first weighed and then given a set of 5 grip strength trials”
Other settings in the paper
Limbs	all four limbs	“we modified this method to include all four limbs”
Maximum tensile force range	0 to 50 N	“a maximum tensile force range from 0 to 50 N”
Reading accuracy	0.1 g steps	“a reading accuracy of 0.1 g steps”
Error	≤ 0.3 g	“an error of ≤ 0.3 g”
Grid	steel grid	“both the fore and hind paws could grasp the steel grid”
Gently pulled away from the apparatus by the tail	until the grip was released	“gently pulled away from the apparatus by the tail until the grip was released”
Objective of the assessment	Peak force	“Peak force was the objective of the assessment”
Fitness testing	once per month	“All participants underwent fitness testing once per month over a period of 6 months”
Period	6 months	“All participants underwent fitness testing once per month over a period of 6 months”
Grip strength and rotarod tests were performed	on the same day	“Grip strength and rotarod tests were performed on the same day”
Apparatus
Maze Engineers Grip Strength Instrument		“Grip strength was recorded via Maze Engineers Grip Strength Instrument (Skokie, IL, USA)”
Methods cited to other work
The battery the fitness tests were derived from, the comprehensive functional assessment battery (CFAB)	[18]	“Fitness tests were derived from the comprehensive functional assessment battery (CFAB), a set of noninvasive tests used to assess and compare the physical function and motor task ability of rodents [18]”

Rotarod test

Compare with 9 other papers

Setting	Value	Paper’s words
Rod speed	Starting 4 revolutions/min Maximum speed 40 revolutions/min	“rotation starting at 4 revolutions/min” “until a maximum speed of 40 revolutions/min”
Trial duration	5 min	“Testing consisted of 5 min maximum latency”
Number of trials	3 trials	“On testing days, mice performed 3 trials of rotarod”
Other settings in the paper
Increasing steadily	7.2 revolutions/min^2	“increasing steadily at 7.2 revolutions/min^2”
Break	Between trials >10 min	“a break of>10 min between trials”
Rotating dowels	In half the lanes 50% smaller	“50% smaller rotating dowels in half the lanes”
Latency	automatically recorded via the motion sensor on the apparatus	“their latency was automatically recorded via the motion sensor on the apparatus”
Fitness testing	once per month	“All participants underwent fitness testing once per month over a period of 6 months”
Period	6 months	“All participants underwent fitness testing once per month over a period of 6 months”
Grip strength and rotarod tests were performed	on the same day	“Grip strength and rotarod tests were performed on the same day”
Apparatus
Maze Engineers Rotarod Instrument		“Rotarod testing was recorded utilizing Maze Engineers Rotarod Instrument (Skokie, IL, USA)”
Methods cited to other work
The battery the fitness tests were derived from, the comprehensive functional assessment battery (CFAB), and the use of rotarod as a performance and behavioral task	[18]	“Fitness tests were derived from the comprehensive functional assessment battery (CFAB), a set of noninvasive tests used to assess and compare the physical function and motor task ability of rodents [18]”