Sex-specific effects of alcohol on neurobehavioral performance and endoplasmic reticulum stress: an analysis using neuron-specific MANF deficient mice

Wen W, Li H, Lauffer M, et al. Frontiers in pharmacology 2024;15:1407576.

The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made

Balance beam test

Setting	Value	Paper’s words
Beam size	80 cm-long Beam diameters 12 mm Beam diameters 6 mm	“An 80 cm-long beam is elevated 50 cm from the floor” “Two different beam diameters were used: 12 mm and 6 mm”
Beam height	50 cm	“An 80 cm-long beam is elevated 50 cm from the floor”
Number of trials	Trained three trials per beam width each day Tested three trials per beam width	“Each animal was trained for two consecutive days to cross the beam three trials per beam width each day” “Each animal was tested for three trials per beam width with 5–10 min of rest between trials”
Other settings in the paper
Safe zone	containing clean bedding and food	“A safe zone is attached to one end of the beam, containing clean bedding and food”
Trained for	two consecutive days	“Each animal was trained for two consecutive days”
Tested for	1 day	“and then tested for 1 day”
Rest between trials	Tested 5–10 min	“Each animal was tested for three trials per beam width with 5–10 min of rest between trials”
First tested on	During the test day the 12 mm diameter beam and then the 6 mm diameter beam	“During the test day, mice were first tested on the 12 mm diameter beam and then the 6 mm diameter beam”
Prompt	During training day 1 its tail was gently prompt to encourage movement	“During training day 1, if an animal stopped crossing the beam, its tail was gently prompt to encourage movement”
Prompting	Training day 2 and on the testing day No prompting	“No prompting was performed during training day 2 and on the testing day”
Fell from the beam	placed back on the end of the beam to restart crossing	“If an animal fell from the beam, it was placed back on the end of the beam to restart crossing”
Pause	less than 5 s	“A pause is defined as the mouse pauses for less than 5 s and then proceeds without prompting”
Stall	more than 5 s	“A stall is defined as the mouse pauses for more than 5 s and then proceeds without prompting”
Invalid	A trial with stall	“A trial with stall was invalid and cannot be scored for the latency to cross”
Invalid	falls or stalls for more than 5 s and will not proceed without prompting	“A trial was also invalid if the mouse falls or stalls for more than 5 s and will not proceed without prompting”
Divided into	two batches	“animals were randomly divided into two batches and tested separately for balance beam, DigiGait, and Barnes maze tests”
Order of tests	Batch 1 Day 29–31	“Day 29–31, balance beam for batch 1”
Order of tests	Batch 2 Day 36–38	“Day 36–38, balance beam for batch 2”
Rest in between tests	at least a 1-day	“Animals were given at least a 1-day rest in between tests”
Apparatus
Balance beam	Conduct Science MazeEngineers	“Balance beam test (Conduct Science MazeEngineers, Skokie, IL)”
Front camera		“Mice behavior were recorded using a front camera and an overhead camera”
Overhead camera		“Mice behavior were recorded using a front camera and an overhead camera”

Elevated plus maze test

Compare with 3 other papers

Setting	Value	Paper’s words
Session duration	5 min	“Each animal was placed in the central area then activity was recorded for 5 min”
Illumination	In the open arms 215 lux	“an 18″ ring light (Inkeltech) mounted around overhead camera that generates 215 lux of light in the open arms”
Not in the Methods	Arm size, maze elevation
Other settings in the paper
Habituated in the testing room	at least 30 min	“All animals were habituated in the testing room at least 30 min before testing starts”
Ring light	18″	“an 18″ ring light (Inkeltech) mounted around overhead camera”
Placed	in the central area	“Each animal was placed in the central area”
Order of tests	Day 5	“Day 5, elevated plus maze”
Rest in between tests	at least a 1-day	“Animals were given at least a 1-day rest in between tests”
Apparatus
EthoVision XT 17 system	Noldus Information Technology	“EthoVision XT 17 system (Noldus Information Technology, Wageningen, Netherlands)”
Elevated plus maze	San Diego Instruments	“the elevated plus maze (San Diego Instruments, San Diego, CA)”
18″ ring light	Inkeltech	“an 18″ ring light (Inkeltech) mounted around overhead camera”
Overhead camera		“an 18″ ring light (Inkeltech) mounted around overhead camera”

Gait analysis

Compare with 8 other papers

Setting	Value	Paper’s words
Run duration criterion	Unclear within 5 s The 5 s is a time window for 10 consecutive strides in a treadmill video. It limits which videos were analyzed, but the passage does not call it a limit on run duration.	“Videos with at least 10 consecutive strides within 5 s were analyzed using the DigiGait software”
Not in the Methods	Walkway size, number of runs
Other settings in the paper
Speeds	10 cm/s, 20 cm/s, and 30 cm/s	“Each mouse was tested on three speeds: 10 cm/s, 20 cm/s, and 30 cm/s”
Acclimated in the treadmill compartment	1 min	“Animal was acclimated in the treadmill compartment for 1 min”
Low speed	5 cm/s	“treadmill was turned on at a low speed (5 cm/s)”
Gradually increased to the testing speed	over several seconds	“gradually increased to the testing speed over several seconds”
High-speed video camera	positioned below the transparent belt	“a high-speed video camera positioned below the transparent belt”
Consecutive strides	at least 10	“Videos with at least 10 consecutive strides within 5 s were analyzed using the DigiGait software”
Unable to walk at the target speed	removed from the study	“Animals that were unable to walk at the target speed were removed from the study”
Divided into	two batches	“animals were randomly divided into two batches and tested separately for balance beam, DigiGait, and Barnes maze tests”
Order of tests	Batch 1 Day 33	“Day 33, DigiGait for batch 1”
Order of tests	Batch 2 Day 40	“Day 40, DigiGait for batch 2”
Rest in between tests	at least a 1-day	“Animals were given at least a 1-day rest in between tests”
Apparatus
DigiGait Imaging System	Mouse Specifics	“the DigiGait Imaging System (Mouse Specifics, Framingham, MA)”
DigiGait treadmill		“Videos of the mice walked on the DigiGait treadmill”
High-speed video camera		“a high-speed video camera positioned below the transparent belt”
DigiGait software		“were analyzed using the DigiGait software”

Open field test

Compare with 10 other papers

Setting	Value	Paper’s words
Arena size	16″×16.5″×12″	“a custom made chamber (16″×16.5″×12″)”
Session duration	10 min	“Activity was recorded for 10 min for each animal once a day for two consecutive days”
Illumination	110–130 lux	“All testing was conducted under 110–130 lux of lighting across the chamber”
Center zone size	a length and width half the length and width of the chamber	“The center of the open field was defined as the central zone with a length and width half the length and width of the chamber”
Other settings in the paper
Habituated in the testing room	at least 30 min	“All animals were habituated in the testing room at least 30 min before testing starts”
Activity was recorded	once a day for two consecutive days	“Activity was recorded for 10 min for each animal once a day for two consecutive days”
Placed	in the center	“Each mouse was placed in the center of a custom made chamber”
Chamber	opaque black walls and white floor	“a custom made chamber (16″×16.5″×12″) with opaque black walls and white floor”
Order of tests	Day 1–2	“Day 1–2, open field”
Rest in between tests	at least a 1-day	“Animals were given at least a 1-day rest in between tests”
Apparatus
EthoVision XT 17 system	Noldus Information Technology	“EthoVision XT 17 system (Noldus Information Technology, Wageningen, Netherlands)”
Custom made chamber		“a custom made chamber (16″×16.5″×12″)”

Rotarod test

Compare with 9 other papers

Setting	Value	Paper’s words
Rod speed	Training trial four revolutions per minute (rpm) Testing trial 4 to 40 rpm	“For the training trial, mice were placed on the rotarod with a constant speed of four revolutions per minute (rpm) for 5 min” “For the testing trial, the rotarod was set to accelerate from 4 to 40 rpm over the course of 5 min”
Trial duration	Training trial 5 min Testing trial Unclear 5 min The 5 min is printed as the time the rod takes to accelerate. The passage does not say the testing trial stops at 5 min.	“For the training trial, mice were placed on the rotarod with a constant speed of four revolutions per minute (rpm) for 5 min” “For the testing trial, the rotarod was set to accelerate from 4 to 40 rpm over the course of 5 min”
Number of trials	Testing trial three testing trials	“Each mouse was tested for three testing trials with 10–15 min to rest in between trials”
Other settings in the paper
Accelerate from 4 to 40 rpm over the course of	Testing trial 5 min	“For the testing trial, the rotarod was set to accelerate from 4 to 40 rpm over the course of 5 min”
Rest in between trials	Testing trials 10–15 min	“Each mouse was tested for three testing trials with 10–15 min to rest in between trials”
Order of tests	Day 10	“Day 10, rotarod”
Rest in between tests	at least a 1-day	“Animals were given at least a 1-day rest in between tests”
Apparatus
Accelerating rotarod	IITC Life Science	“the accelerating rotarod (IITC Life Science, Woodland Hills, CA)”

Compare with 6 other papers

Setting	Value	Paper’s words
Session duration	Habituation session 10 min Testing session 10 min	“During the habituation session, cylinders in the two side chambers were empty” “allowed to explore the arena freely for 10 min” “Then it is followed with 10 min testing session”
Other settings in the paper
Acclimated in the testing room	at least 30 min	“All animals were acclimated in the testing room at least 30 min before testing starts”
Chambers	three	“a custom made matte black plastic box arena with three chambers and openings between the chambers”
Plastic cylinder	with holes	“The two side chambers contain a plastic cylinder with holes”
Placed	in the middle chamber	“An animal was placed in the middle chamber”
Cylinders	Habituation session empty	“During the habituation session, cylinders in the two side chambers were empty”
Cylinder	Testing session a novel mouse was placed in the cylinder in one chamber and a novel object was placed in the cylinder in the other chamber	“Then it is followed with 10 min testing session, during which a novel mouse was placed in the cylinder in one chamber and a novel object was placed in the cylinder in the other chamber”
Order of tests	Day 8	“Day 8, three-chamber sociability task”
Rest in between tests	at least a 1-day	“Animals were given at least a 1-day rest in between tests”
Apparatus
EthoVision XT 17 system	Noldus Information Technology	“EthoVision XT 17 system (Noldus Information Technology, Wageningen, Netherlands)”
Custom made matte black plastic box arena		“a custom made matte black plastic box arena with three chambers and openings between the chambers”
Plastic cylinder		“The two side chambers contain a plastic cylinder with holes”

Sex-specific effects of alcohol on neurobehavioral performance and endoplasmic reticulum stress: an analysis using neuron-specific MANF deficient mice

Wen W, Li H, Lauffer M, et al. Frontiers in pharmacology 2024;15:1407576.

The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made

Balance beam test

Compare with 4 other papers

Setting	Value	Paper’s words
Beam size	80 cm-long Beam diameters 12 mm Beam diameters 6 mm	“An 80 cm-long beam is elevated 50 cm from the floor” “Two different beam diameters were used: 12 mm and 6 mm”
Beam height	50 cm	“An 80 cm-long beam is elevated 50 cm from the floor”
Number of trials	Trained three trials per beam width each day Tested three trials per beam width	“Each animal was trained for two consecutive days to cross the beam three trials per beam width each day” “Each animal was tested for three trials per beam width with 5–10 min of rest between trials”
Other settings in the paper
Safe zone	containing clean bedding and food	“A safe zone is attached to one end of the beam, containing clean bedding and food”
Trained for	two consecutive days	“Each animal was trained for two consecutive days”
Tested for	1 day	“and then tested for 1 day”
Rest between trials	Tested 5–10 min	“Each animal was tested for three trials per beam width with 5–10 min of rest between trials”
First tested on	During the test day the 12 mm diameter beam and then the 6 mm diameter beam	“During the test day, mice were first tested on the 12 mm diameter beam and then the 6 mm diameter beam”
Prompt	During training day 1 its tail was gently prompt to encourage movement	“During training day 1, if an animal stopped crossing the beam, its tail was gently prompt to encourage movement”
Prompting	Training day 2 and on the testing day No prompting	“No prompting was performed during training day 2 and on the testing day”
Fell from the beam	placed back on the end of the beam to restart crossing	“If an animal fell from the beam, it was placed back on the end of the beam to restart crossing”
Pause	less than 5 s	“A pause is defined as the mouse pauses for less than 5 s and then proceeds without prompting”
Stall	more than 5 s	“A stall is defined as the mouse pauses for more than 5 s and then proceeds without prompting”
Invalid	A trial with stall	“A trial with stall was invalid and cannot be scored for the latency to cross”
Invalid	falls or stalls for more than 5 s and will not proceed without prompting	“A trial was also invalid if the mouse falls or stalls for more than 5 s and will not proceed without prompting”
Divided into	two batches	“animals were randomly divided into two batches and tested separately for balance beam, DigiGait, and Barnes maze tests”
Order of tests	Batch 1 Day 29–31	“Day 29–31, balance beam for batch 1”
Order of tests	Batch 2 Day 36–38	“Day 36–38, balance beam for batch 2”
Rest in between tests	at least a 1-day	“Animals were given at least a 1-day rest in between tests”
Apparatus
Balance beam	Conduct Science MazeEngineers	“Balance beam test (Conduct Science MazeEngineers, Skokie, IL)”
Front camera		“Mice behavior were recorded using a front camera and an overhead camera”
Overhead camera		“Mice behavior were recorded using a front camera and an overhead camera”

Elevated plus maze test

Compare with 3 other papers

Setting	Value	Paper’s words
Session duration	5 min	“Each animal was placed in the central area then activity was recorded for 5 min”
Illumination	In the open arms 215 lux	“an 18″ ring light (Inkeltech) mounted around overhead camera that generates 215 lux of light in the open arms”
Not in the Methods	Arm size, maze elevation
Other settings in the paper
Habituated in the testing room	at least 30 min	“All animals were habituated in the testing room at least 30 min before testing starts”
Ring light	18″	“an 18″ ring light (Inkeltech) mounted around overhead camera”
Placed	in the central area	“Each animal was placed in the central area”
Order of tests	Day 5	“Day 5, elevated plus maze”
Rest in between tests	at least a 1-day	“Animals were given at least a 1-day rest in between tests”
Apparatus
EthoVision XT 17 system	Noldus Information Technology	“EthoVision XT 17 system (Noldus Information Technology, Wageningen, Netherlands)”
Elevated plus maze	San Diego Instruments	“the elevated plus maze (San Diego Instruments, San Diego, CA)”
18″ ring light	Inkeltech	“an 18″ ring light (Inkeltech) mounted around overhead camera”
Overhead camera		“an 18″ ring light (Inkeltech) mounted around overhead camera”

Gait analysis

Compare with 8 other papers

Setting	Value	Paper’s words
Run duration criterion	Unclear within 5 s The 5 s is a time window for 10 consecutive strides in a treadmill video. It limits which videos were analyzed, but the passage does not call it a limit on run duration.	“Videos with at least 10 consecutive strides within 5 s were analyzed using the DigiGait software”
Not in the Methods	Walkway size, number of runs
Other settings in the paper
Speeds	10 cm/s, 20 cm/s, and 30 cm/s	“Each mouse was tested on three speeds: 10 cm/s, 20 cm/s, and 30 cm/s”
Acclimated in the treadmill compartment	1 min	“Animal was acclimated in the treadmill compartment for 1 min”
Low speed	5 cm/s	“treadmill was turned on at a low speed (5 cm/s)”
Gradually increased to the testing speed	over several seconds	“gradually increased to the testing speed over several seconds”
High-speed video camera	positioned below the transparent belt	“a high-speed video camera positioned below the transparent belt”
Consecutive strides	at least 10	“Videos with at least 10 consecutive strides within 5 s were analyzed using the DigiGait software”
Unable to walk at the target speed	removed from the study	“Animals that were unable to walk at the target speed were removed from the study”
Divided into	two batches	“animals were randomly divided into two batches and tested separately for balance beam, DigiGait, and Barnes maze tests”
Order of tests	Batch 1 Day 33	“Day 33, DigiGait for batch 1”
Order of tests	Batch 2 Day 40	“Day 40, DigiGait for batch 2”
Rest in between tests	at least a 1-day	“Animals were given at least a 1-day rest in between tests”
Apparatus
DigiGait Imaging System	Mouse Specifics	“the DigiGait Imaging System (Mouse Specifics, Framingham, MA)”
DigiGait treadmill		“Videos of the mice walked on the DigiGait treadmill”
High-speed video camera		“a high-speed video camera positioned below the transparent belt”
DigiGait software		“were analyzed using the DigiGait software”

Open field test

Compare with 10 other papers

Setting	Value	Paper’s words
Arena size	16″×16.5″×12″	“a custom made chamber (16″×16.5″×12″)”
Session duration	10 min	“Activity was recorded for 10 min for each animal once a day for two consecutive days”
Illumination	110–130 lux	“All testing was conducted under 110–130 lux of lighting across the chamber”
Center zone size	a length and width half the length and width of the chamber	“The center of the open field was defined as the central zone with a length and width half the length and width of the chamber”
Other settings in the paper
Habituated in the testing room	at least 30 min	“All animals were habituated in the testing room at least 30 min before testing starts”
Activity was recorded	once a day for two consecutive days	“Activity was recorded for 10 min for each animal once a day for two consecutive days”
Placed	in the center	“Each mouse was placed in the center of a custom made chamber”
Chamber	opaque black walls and white floor	“a custom made chamber (16″×16.5″×12″) with opaque black walls and white floor”
Order of tests	Day 1–2	“Day 1–2, open field”
Rest in between tests	at least a 1-day	“Animals were given at least a 1-day rest in between tests”
Apparatus
EthoVision XT 17 system	Noldus Information Technology	“EthoVision XT 17 system (Noldus Information Technology, Wageningen, Netherlands)”
Custom made chamber		“a custom made chamber (16″×16.5″×12″)”

Rotarod test

Compare with 9 other papers

Setting	Value	Paper’s words
Rod speed	Training trial four revolutions per minute (rpm) Testing trial 4 to 40 rpm	“For the training trial, mice were placed on the rotarod with a constant speed of four revolutions per minute (rpm) for 5 min” “For the testing trial, the rotarod was set to accelerate from 4 to 40 rpm over the course of 5 min”
Trial duration	Training trial 5 min Testing trial Unclear 5 min The 5 min is printed as the time the rod takes to accelerate. The passage does not say the testing trial stops at 5 min.	“For the training trial, mice were placed on the rotarod with a constant speed of four revolutions per minute (rpm) for 5 min” “For the testing trial, the rotarod was set to accelerate from 4 to 40 rpm over the course of 5 min”
Number of trials	Testing trial three testing trials	“Each mouse was tested for three testing trials with 10–15 min to rest in between trials”
Other settings in the paper
Accelerate from 4 to 40 rpm over the course of	Testing trial 5 min	“For the testing trial, the rotarod was set to accelerate from 4 to 40 rpm over the course of 5 min”
Rest in between trials	Testing trials 10–15 min	“Each mouse was tested for three testing trials with 10–15 min to rest in between trials”
Order of tests	Day 10	“Day 10, rotarod”
Rest in between tests	at least a 1-day	“Animals were given at least a 1-day rest in between tests”
Apparatus
Accelerating rotarod	IITC Life Science	“the accelerating rotarod (IITC Life Science, Woodland Hills, CA)”

Compare with 6 other papers

Setting	Value	Paper’s words
Session duration	Habituation session 10 min Testing session 10 min	“During the habituation session, cylinders in the two side chambers were empty” “allowed to explore the arena freely for 10 min” “Then it is followed with 10 min testing session”
Other settings in the paper
Acclimated in the testing room	at least 30 min	“All animals were acclimated in the testing room at least 30 min before testing starts”
Chambers	three	“a custom made matte black plastic box arena with three chambers and openings between the chambers”
Plastic cylinder	with holes	“The two side chambers contain a plastic cylinder with holes”
Placed	in the middle chamber	“An animal was placed in the middle chamber”
Cylinders	Habituation session empty	“During the habituation session, cylinders in the two side chambers were empty”
Cylinder	Testing session a novel mouse was placed in the cylinder in one chamber and a novel object was placed in the cylinder in the other chamber	“Then it is followed with 10 min testing session, during which a novel mouse was placed in the cylinder in one chamber and a novel object was placed in the cylinder in the other chamber”
Order of tests	Day 8	“Day 8, three-chamber sociability task”
Rest in between tests	at least a 1-day	“Animals were given at least a 1-day rest in between tests”
Apparatus
EthoVision XT 17 system	Noldus Information Technology	“EthoVision XT 17 system (Noldus Information Technology, Wageningen, Netherlands)”
Custom made matte black plastic box arena		“a custom made matte black plastic box arena with three chambers and openings between the chambers”
Plastic cylinder		“The two side chambers contain a plastic cylinder with holes”

Balance beam test

Elevated plus maze test

Gait analysis

Open field test

Rotarod test

Social interaction test

Balance beam test

Elevated plus maze test

Gait analysis

Open field test

Rotarod test

Social interaction test