Liu Y, Wang Y, Zhao ZD, et al. Nature metabolism 2024;6(8):1616-1631.
The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made
| Setting | Value | Paper’s words |
|---|---|---|
| Arm size |
| “with four arms (each 30 cm in length and 5 cm in width), two opposing closed arms with 14 cm walls and two opposing open arms” “two opposing closed arms with 14 cm walls and two opposing open arms” |
| Maze elevation |
| “The EPM apparatus consisted of an elevated platform (80 cm above the floor)” |
| Session duration |
| “The trajectories of the mice were tracked for 5 min” |
| Not in the Methods | Illumination | |
| Other settings in the paper | ||
| Environmental habituation | at least 30 min | “mice were brought to the testing room for environmental habituation for at least 30 min” |
| Start position | in the center of the EPM apparatus, towards one of the open arms | “Mice were individually placed in the center of the EPM apparatus, towards one of the open arms.” |
| Apparatus | ||
| EPM apparatus | “The EPM apparatus consisted of an elevated platform (80 cm above the floor)” | |
| Ethovision video tracking system V14 | Noldus Information Technology | “the time spent in the open arms was recorded with an Ethovision video tracking system V14 (Noldus Information Technology)” |
| Setting | Value | Paper’s words |
|---|---|---|
| Arena size |
| “A clear box (27.3 cm × 27.3 cm square base with 20.3 cm high walls) was used for the open field test” |
| Session duration |
| “Movements were recorded (Med Associates, ENV-510) for 1 h in 5 min bins” |
| Not in the Methods | Illumination, center zone size | |
| Other settings in the paper | ||
| The center zone | 36% of the total area | “the center zone was 36% of the total area” |
| Habituated to the test room | at least 20 min | “Before testing, mice were habituated to the test room for at least 20 min.” |
| Bins | 5 min bins | “Movements were recorded (Med Associates, ENV-510) for 1 h in 5 min bins” |
| Start position | in the center of the box | “Mice were placed in the center of the box at the start of the assay.” |
| Apparatus | ||
| Clear box | “A clear box (27.3 cm × 27.3 cm square base with 20.3 cm high walls) was used for the open field test” | |
| Med Associates, ENV-510 | “Movements were recorded (Med Associates, ENV-510) for 1 h in 5 min bins” | |
| Setting | Value | Paper’s words |
|---|---|---|
| Arena size |
| “Each social interaction chamber (30 × 30 × 30 cm) contained dividing walls with an open middle section to allow access.” |
| Session duration |
| “Mice were given free access to the apparatus for 10 min (in the absence of other mice) to habituate” “For each phase, the test mice explored the entire arena throughout the 10-min trial.” |
| Other settings in the paper | ||
| Close interaction with the nose point within 2 cm of the enclosure | 2 cm | “the time spent in close interaction with the nose point within 2 cm of the enclosure was also recorded” |
| Start position | Sociability the middle chamber of the apparatus | “mice were placed into the middle chamber of the apparatus with one outer chamber containing one mouse ('stranger 1') confined in a wire cup and the other chamber containing a Lego block” |
| Apparatus | ||
| Social interaction chamber | “Each social interaction chamber (30 × 30 × 30 cm) contained dividing walls with an open middle section to allow access.” | |
| Wire cups | “Both outer chambers contained wire cups.” | |
| EthoVision XT 14 | Noldus Information Technology | “the time spent in close interaction with the nose point within 2 cm of the enclosure was also recorded (EthoVision XT 14, Noldus Information Technology)” |
| Methods cited to other work | ||
| Scoring of time spent interacting with the empty wire cup, stranger 1 and stranger 2 | Reference 68 in the paper | “The time spent interacting with the empty wire, stranger 1 and stranger 2 mice during the 10-min session was recorded^68.” |
| Setting | Value | Paper’s words |
|---|---|---|
| The test period | 3 h | “The test period lasted for 3 h” |
| Sucrose solution (w:v) | 2% | “one bottle of drinking water containing 2% sucrose solution (w:v)” |
| Singly housed | at least 2 days | “Mice were singly housed for at least 2 days before the tests.” |
| Habituated with two bottles of water | the first 2 days | “For the first 2 days, animals were habituated with two bottles of water” |
| Position of the two bottles switched | at 24 h | “with the position of the two bottles switched at 24 h” |
| Water-deprived | On the night of day 2 16 h | “On the night of day 2, mice were water-deprived for 16 h and then the test was started on day 3.” |