Genetically diverse mice exhibit divergent domain-specific, sex-dependent behavioral outcomes following exposure to early life stress

Nguyen J, Shimizu K, Zlotnik V, et al. PloS one 2025;20(9):e0331457.

The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made

Forced swim test

Setting	Value	Paper’s words
Water temperature	21–23 °C	“room temperature (21–23 °C) water”
Water depth	2/3 full	“2/3 full with room temperature (21–23 °C) water”
Cylinder size	25 cm diameter × 30 cm height	“polypropylene cylindrical container (25 cm diameter × 30 cm height)”
Session duration	7 min	“For 7 min, each test mouse was allowed to swim freely”
Other settings in the paper
Left undisturbed	90 minutes	“placed it in a clean recovery cage, where it was left undisturbed for 90 minutes”
Apparatus
Polypropylene cylindrical container		“gently placed into a polypropylene cylindrical container (25 cm diameter × 30 cm height)”

Compare with 6 other papers

Setting	Value	Paper’s words
Session duration	25-min	“2pSI was a 25-min session”
Other settings in the paper
Runways	30 × 7 cm	“a T-shaped, opaque acrylic maze consisting of three runways (30 × 7 cm)”
Runways	three	“consisting of three runways”
End-compartments	5 × 7 cm	“two of which included end-compartments (5 × 7 cm)”
Habituated to the maze	5 min	“test mice were first habituated to the maze for 5 min (with no stimuli inside the compartments)”
Social behavior phases	10-min	“two consecutive 10-min social behavior phases”
Social behavior phases	two	“two consecutive 10-min social behavior phases”
Left or right compartment	Phase 1 counterbalanced	“During Phase 1, the test mouse could interact (through the barrier panels) with a same-sex novel conspecific or a novel object (left or right compartment, counterbalanced)”
Test mouse could interact (through the barrier panels) with	Phase 1 a same-sex novel conspecific or a novel object	“During Phase 1, the test mouse could interact (through the barrier panels) with a same-sex novel conspecific or a novel object (left or right compartment, counterbalanced)”
The test mouse could interact with	Phase 2 the original, “familiar” conspecific, or the new “unfamiliar” conspecific	“During Phase 2, the novel object was replaced with another novel same-sex conspecific; the test mouse could interact with the original, “familiar” conspecific, or the new “unfamiliar” conspecific”
Interaction	the time that the center-point of the test animal was tracked inside defined zones adjacent to the stimulus compartments	“Interaction was operationally defined as the time that the center-point of the test animal was tracked inside defined zones adjacent to the stimulus compartments”
Empty end zone	an identical area	“In the non-compartment end of the maze, an identical area was demarcated as the empty end zone”
Apparatus was cleaned	Between test mice unscented disinfectant	“Between test mice, the apparatus was cleaned with an unscented disinfectant (Seventh Generation Inc., USA)”
Each conspecific mouse was used	up to three times per test day	“Each conspecific mouse was used up to three times per test day”
Handled by experimenters	2 min/ day for 3 consecutive days	“mice were handled by experimenters for 2 min/ day for 3 consecutive days prior to being tested behaviorally”
Acclimated to the behavioral testing room	at least 1 h	“mice were acclimated to the behavioral testing room for at least 1 h prior to being tested”
Apparatus
EthoVision XT	Noldus	“Behavior was scored using an automated tracking system, EthoVision XT version 14 (Noldus, Leesburg, VA)”
T-shaped, opaque acrylic maze	TAP Plastics	“a T-shaped, opaque acrylic maze consisting of three runways (30 × 7 cm), two of which included end-compartments (5 × 7 cm) that held a novel conspecific or object (TAP Plastics, San Jose, CA)”
Transparent, air-holed acrylic barrier panels		“The compartment enclosures were created by the insertion of transparent, air-holed acrylic barrier panels”
Novel object		“a same-sex novel conspecific or a novel object”
Unscented disinfectant	Seventh Generation Inc.	“the apparatus was cleaned with an unscented disinfectant (Seventh Generation Inc., USA)”

Genetically diverse mice exhibit divergent domain-specific, sex-dependent behavioral outcomes following exposure to early life stress

Nguyen J, Shimizu K, Zlotnik V, et al. PloS one 2025;20(9):e0331457.

The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made

Forced swim test

Compare with 4 other papers

Setting	Value	Paper’s words
Water temperature	21–23 °C	“room temperature (21–23 °C) water”
Water depth	2/3 full	“2/3 full with room temperature (21–23 °C) water”
Cylinder size	25 cm diameter × 30 cm height	“polypropylene cylindrical container (25 cm diameter × 30 cm height)”
Session duration	7 min	“For 7 min, each test mouse was allowed to swim freely”
Other settings in the paper
Left undisturbed	90 minutes	“placed it in a clean recovery cage, where it was left undisturbed for 90 minutes”
Apparatus
Polypropylene cylindrical container		“gently placed into a polypropylene cylindrical container (25 cm diameter × 30 cm height)”

Compare with 6 other papers

Setting	Value	Paper’s words
Session duration	25-min	“2pSI was a 25-min session”
Other settings in the paper
Runways	30 × 7 cm	“a T-shaped, opaque acrylic maze consisting of three runways (30 × 7 cm)”
Runways	three	“consisting of three runways”
End-compartments	5 × 7 cm	“two of which included end-compartments (5 × 7 cm)”
Habituated to the maze	5 min	“test mice were first habituated to the maze for 5 min (with no stimuli inside the compartments)”
Social behavior phases	10-min	“two consecutive 10-min social behavior phases”
Social behavior phases	two	“two consecutive 10-min social behavior phases”
Left or right compartment	Phase 1 counterbalanced	“During Phase 1, the test mouse could interact (through the barrier panels) with a same-sex novel conspecific or a novel object (left or right compartment, counterbalanced)”
Test mouse could interact (through the barrier panels) with	Phase 1 a same-sex novel conspecific or a novel object	“During Phase 1, the test mouse could interact (through the barrier panels) with a same-sex novel conspecific or a novel object (left or right compartment, counterbalanced)”
The test mouse could interact with	Phase 2 the original, “familiar” conspecific, or the new “unfamiliar” conspecific	“During Phase 2, the novel object was replaced with another novel same-sex conspecific; the test mouse could interact with the original, “familiar” conspecific, or the new “unfamiliar” conspecific”
Interaction	the time that the center-point of the test animal was tracked inside defined zones adjacent to the stimulus compartments	“Interaction was operationally defined as the time that the center-point of the test animal was tracked inside defined zones adjacent to the stimulus compartments”
Empty end zone	an identical area	“In the non-compartment end of the maze, an identical area was demarcated as the empty end zone”
Apparatus was cleaned	Between test mice unscented disinfectant	“Between test mice, the apparatus was cleaned with an unscented disinfectant (Seventh Generation Inc., USA)”
Each conspecific mouse was used	up to three times per test day	“Each conspecific mouse was used up to three times per test day”
Handled by experimenters	2 min/ day for 3 consecutive days	“mice were handled by experimenters for 2 min/ day for 3 consecutive days prior to being tested behaviorally”
Acclimated to the behavioral testing room	at least 1 h	“mice were acclimated to the behavioral testing room for at least 1 h prior to being tested”
Apparatus
EthoVision XT	Noldus	“Behavior was scored using an automated tracking system, EthoVision XT version 14 (Noldus, Leesburg, VA)”
T-shaped, opaque acrylic maze	TAP Plastics	“a T-shaped, opaque acrylic maze consisting of three runways (30 × 7 cm), two of which included end-compartments (5 × 7 cm) that held a novel conspecific or object (TAP Plastics, San Jose, CA)”
Transparent, air-holed acrylic barrier panels		“The compartment enclosures were created by the insertion of transparent, air-holed acrylic barrier panels”
Novel object		“a same-sex novel conspecific or a novel object”
Unscented disinfectant	Seventh Generation Inc.	“the apparatus was cleaned with an unscented disinfectant (Seventh Generation Inc., USA)”