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Papers/Ramme et al. 2025

Behavioral phenotyping identifies autism-like repetitive stereotypies in a Tsc2 haploinsufficient rat model

Ramme A, Zachow M, Habelt B, et al. Behavioral and brain functions : BBF 2025;21(1):20.

The test records were read once by AI and checked by code. No person has reviewed them yet. How records are made

Tests in this paper

  • Forced swim test
  • Marble burying test

Forced swim test

Compare with 4 other papers
SettingValuePaper’s words
Water temperature
  • For habituation

    23 ± 1 °C

“For habituation, animals were placed in a glass cylinder (60 cm height, 24 cm diameter) filled with 23 ± 1 °C water”
Water depth
  • For habituation

    30 cm

  • After 24 h, animals were tested

    40 cm

“For habituation, animals were placed in a glass cylinder (60 cm height, 24 cm diameter) filled with 23 ± 1 °C water to 30 cm height”
“After 24 h, animals were tested in a water height of 40 cm”
Cylinder size
  • For habituation

    60 cm height, 24 cm diameter

“For habituation, animals were placed in a glass cylinder (60 cm height, 24 cm diameter)”
Session duration
  • For habituation

    15 min

  • After 24 h, animals were tested

    5 min

“For habituation, animals were placed in a glass cylinder (60 cm height, 24 cm diameter) filled with 23 ± 1 °C water to 30 cm height for 15 min individually”
“After 24 h, animals were tested in a water height of 40 cm, and activity was recorded for 5 min”
Other settings in the paper
Animals were placed in a glass cylinder

For habituation

individually

“For habituation, animals were placed in a glass cylinder (60 cm height, 24 cm diameter) filled with 23 ± 1 °C water to 30 cm height for 15 min individually”
Dried under a red-light source

Afterward

SIL-6 red light lamp

“Afterward, animals were dried under a red-light source (SIL-6 red light lamp Beurer, Hans Dinslage GmbH, Germany)”
After 24 h, animals were tested

24 h

“After 24 h, animals were tested”
Sequence

fixed sequence depicted in Fig. 1

“Behavioral tests were performed in a fixed sequence depicted in Fig. 1”
Resting period between tests

a minimum of 24 h

“a minimum of 24 h resting period between tests”
Apparatus
Glass cylinder
“a glass cylinder (60 cm height, 24 cm diameter)”
Red-light source

Beurer, Hans Dinslage GmbH, Germany, SIL-6 red light lamp

“dried under a red-light source (SIL-6 red light lamp Beurer, Hans Dinslage GmbH, Germany)”
EthoVision^® XT

Noldus Information Technology, Netherlands

“using the software EthoVision^® XT (Noldus Information Technology, Netherlands)”
Methods cited to other work
How immobility was scored in EthoVision; the criteria are not printed

[45, 46]

“The total time of immobility per animal was determined using the software EthoVision^® XT (Noldus Information Technology, Netherlands) [45, 46]”

Marble burying test

Compare with 2 other papers
SettingValuePaper’s words
Session duration
  • 30 min

“Animals were allowed to explore for 30 min”
Other settings in the paper
Animals were placed

individually

“Animals were placed individually in a standard cage”
Bedding material

5 cm

“bedding material filled up to 5 cm height”
Glass marbles

9

“9 clean, commercially available glass marbles”
Diameter

approx. 15 mm

“glass marbles (approx. 15 mm diameter)”
Glass marbles

evenly distributed on half of the cage´s surface

“glass marbles (approx. 15 mm diameter) were evenly distributed on half of the cage´s surface”
Food and water supply

without food and water supply

“explore for 30 min without food and water supply”
Buried marbles

at least 2/3 covered in bedding material

“the number of buried marbles that were at least 2/3 covered in bedding material was counted”
Sequence

fixed sequence depicted in Fig. 1

“Behavioral tests were performed in a fixed sequence depicted in Fig. 1”
Resting period between tests

a minimum of 24 h

“a minimum of 24 h resting period between tests”
Apparatus
Standard cage
“placed individually in a standard cage containing bedding material”
Bedding material
“a standard cage containing bedding material”
Glass marbles
“9 clean, commercially available glass marbles (approx. 15 mm diameter)”

Tests in this paper

  • Forced swim test
  • Marble burying test

The paper

Species: rats

“All experiments were performed in the heterozygous Eker rat strain”
Read the paper (doi:10.1186/s12993-025-00284-z)Full text on PubMed Central (PMC12225118)

Quotes are shown under the paper’s CC BY license.

How far this was checked

The test records were read once by AI and checked by code. No person has reviewed them yet.

How records are made