Red-shifted channelrhodopsin stimulation restores light responses in blind mice, macaque retina, and human retina

Sengupta A, Chaffiol A, Macé E, et al. EMBO molecular medicine 2016;8(11):1248-1264.

The test records were read three times by AI and checked by code. No person has reviewed them yet. How records are made

Open field test

Setting	Value	Paper’s words
Arena size	diameter 36 cm	“A circular open‐field arena was constructed in house with diameter 36 cm”
Session duration	In the dark 2 min After light was switched on 2 min	“Their activity was recorded for 2 min followed by 2 min of illumination of the chamber” “tracked for 2 min in the dark and for 2 min after light was switched on”
Illumination	Upon LED illumination ~10^15 photons cm^−2 s^−1	“The irradiance of the open field upon LED illumination was ~10^15 photons cm^−2 s^−1”
Other settings in the paper
Dark adapted	30 min	“blind rd1 control mice were dark adapted for 30 min before being placed in the center”
Placed	in the center	“blind rd1 control mice were dark adapted for 30 min before being placed in the center”
LED light sources	590‐nm	“Ten 590‐nm LED light sources”
590‐nm LED light sources	Ten	“Ten 590‐nm LED light sources”
Video camera	night vision mode	“Activity in the arena was recorded during the test by a video camera (Handycam, Sony Corporation, Tokyo, Japan) in night vision mode”
Run during the dark phase of the mice	between 18 and 21 h	“All behavior experiments were run during the dark phase of the mice between 18 and 21 h”
Apparatus
Circular open‐field arena		“A circular open‐field arena was constructed in house with diameter 36 cm using an aluminum sheet folded in a circle placed over a plexiglass base (similar to the one described in Polosukhina et al, 2012).”
590‐nm LED light sources	Lumitronix, Graefelfing, Germany, Cree XP‐E	“Ten 590‐nm LED light sources (Cree XP‐E, Lumitronix, Graefelfing, Germany) were glued to the wall of the open‐field arena.”
Handheld spectrophotometer	Thorlabs, Germany, PM100D	“as measured approximately at the level of the mice's eyes using a handheld spectrophotometer (PM100D, Thorlabs, Germany)”
Video camera	Sony Corporation, Tokyo, Japan, Handycam	“Activity in the arena was recorded during the test by a video camera (Handycam, Sony Corporation, Tokyo, Japan) in night vision mode.”
Ethovision XT	Noldus Information Technology, Leesburg, VA	“The movies were analyzed using mouse behavior phenotyping and motion tracking software Ethovision XT build 8.0 (Noldus Information Technology, Leesburg, VA).”
Methods cited to other work
Construction of the circular open-field arena (described as similar to a published arena)	Polosukhina et al, 2012	“similar to the one described in Polosukhina et al, 2012”
Dark-then-illumination recording protocol (stated as based on a published protocol)	Lagali et al, 2008	“based on the protocol used in Lagali et al, 2008”

Red-shifted channelrhodopsin stimulation restores light responses in blind mice, macaque retina, and human retina

Sengupta A, Chaffiol A, Macé E, et al. EMBO molecular medicine 2016;8(11):1248-1264.

The test records were read three times by AI and checked by code. No person has reviewed them yet. How records are made

Open field test

Compare with 10 other papers

Setting	Value	Paper’s words
Arena size	diameter 36 cm	“A circular open‐field arena was constructed in house with diameter 36 cm”
Session duration	In the dark 2 min After light was switched on 2 min	“Their activity was recorded for 2 min followed by 2 min of illumination of the chamber” “tracked for 2 min in the dark and for 2 min after light was switched on”
Illumination	Upon LED illumination ~10^15 photons cm^−2 s^−1	“The irradiance of the open field upon LED illumination was ~10^15 photons cm^−2 s^−1”
Other settings in the paper
Dark adapted	30 min	“blind rd1 control mice were dark adapted for 30 min before being placed in the center”
Placed	in the center	“blind rd1 control mice were dark adapted for 30 min before being placed in the center”
LED light sources	590‐nm	“Ten 590‐nm LED light sources”
590‐nm LED light sources	Ten	“Ten 590‐nm LED light sources”
Video camera	night vision mode	“Activity in the arena was recorded during the test by a video camera (Handycam, Sony Corporation, Tokyo, Japan) in night vision mode”
Run during the dark phase of the mice	between 18 and 21 h	“All behavior experiments were run during the dark phase of the mice between 18 and 21 h”
Apparatus
Circular open‐field arena		“A circular open‐field arena was constructed in house with diameter 36 cm using an aluminum sheet folded in a circle placed over a plexiglass base (similar to the one described in Polosukhina et al, 2012).”
590‐nm LED light sources	Lumitronix, Graefelfing, Germany, Cree XP‐E	“Ten 590‐nm LED light sources (Cree XP‐E, Lumitronix, Graefelfing, Germany) were glued to the wall of the open‐field arena.”
Handheld spectrophotometer	Thorlabs, Germany, PM100D	“as measured approximately at the level of the mice's eyes using a handheld spectrophotometer (PM100D, Thorlabs, Germany)”
Video camera	Sony Corporation, Tokyo, Japan, Handycam	“Activity in the arena was recorded during the test by a video camera (Handycam, Sony Corporation, Tokyo, Japan) in night vision mode.”
Ethovision XT	Noldus Information Technology, Leesburg, VA	“The movies were analyzed using mouse behavior phenotyping and motion tracking software Ethovision XT build 8.0 (Noldus Information Technology, Leesburg, VA).”
Methods cited to other work
Construction of the circular open-field arena (described as similar to a published arena)	Polosukhina et al, 2012	“similar to the one described in Polosukhina et al, 2012”
Dark-then-illumination recording protocol (stated as based on a published protocol)	Lagali et al, 2008	“based on the protocol used in Lagali et al, 2008”