Accelerating Rotarod Test
Objective: Measurement of latency to fall and balance performance in exercised versus non-exercised mice using an accelerating rotarod test
This is a Accelerating Rotarod Test protocol using mouse as the model organism. The procedure involves 4 procedural steps, 2 equipment items, 2 materials. Extracted from a 2007 paper published in Journal of Neuroscience.
Model and subjects
mouse • C57BL/6J • unknown • Not specified • Not specified
Study window
~4 week study window
Core workflow
Animal grouping and MPTP lesioning • Exercise initiation • Treadmill exercise training
Primary readouts
- Latency to fall on accelerating rotarod
- Balance performance
- Motor velocity on treadmill
- Striatal dopamine tissue levels (measured by HPLC)
Key equipment and reagents
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Protocol Steps
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Animal grouping and MPTP lesioning
C57BL/6J mice were divided into four groups: (1) saline, (2) saline plus exercise, (3) MPTP, and (4) MPTP plus exercise
Note: MPTP lesioning was performed at baseline
View evidence from paper
“C57BL/6J mice were divided into four groups: (1) saline, (2) saline plus exercise, (3) MPTP, and (4) MPTP plus exercise”
Exercise initiation
Exercise was started 5 days after MPTP lesioning
Note: Exercise was initiated after a 5-day recovery period
View evidence from paper
“Exercise was started 5 d after MPTP lesioning”
Treadmill exercise training
Intensive treadmill exercise was conducted on exercised groups
Note: Exercise continued for 28 days total
View evidence from paper
“Exercise was started 5 d after MPTP lesioning and continued for 28 d”
Accelerating rotarod testing
All mice were tested on the accelerating rotarod to measure latency to fall and balance performance
Note: Testing was performed to compare exercised versus non-exercised mice
View evidence from paper
“All exercised animals also showed increased latency to fall (improved balance) using the accelerating rotarod compared with nonexercised mice”