Accelerating Rotarod Test
Objective: Measurement of latency to fall and balance performance in exercised versus non-exercised mice using an accelerating rotarod test
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Equipment2
Materials2
Software1
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Protocol Steps
Animal grouping and MPTP lesioning
C57BL/6J mice were divided into four groups: (1) saline, (2) saline plus exercise, (3) MPTP, and (4) MPTP plus exercise
Note: MPTP lesioning was performed at baseline
View evidence from paper
“C57BL/6J mice were divided into four groups: (1) saline, (2) saline plus exercise, (3) MPTP, and (4) MPTP plus exercise”
Exercise initiation
Exercise was started 5 days after MPTP lesioning
Note: Exercise was initiated after a 5-day recovery period
View evidence from paper
“Exercise was started 5 d after MPTP lesioning”
Treadmill exercise training
Intensive treadmill exercise was conducted on exercised groups
Note: Exercise continued for 28 days total
View evidence from paper
“Exercise was started 5 d after MPTP lesioning and continued for 28 d”
Accelerating rotarod testing
All mice were tested on the accelerating rotarod to measure latency to fall and balance performance
Note: Testing was performed to compare exercised versus non-exercised mice
View evidence from paper
“All exercised animals also showed increased latency to fall (improved balance) using the accelerating rotarod compared with nonexercised mice”