Source Paper
Genetic Variant of BDNF (Val66Met) Polymorphism Attenuates Stroke-Induced Angiogenic Responses by Enhancing Anti-Angiogenic Mediator CD36 Expression
Luye Qin, Eunhee Kim, Rajiv Ratan, Francis S. Lee, Sunghee Cho
Journal of Neuroscience • 2011
Stroke Model with Infarct Size Assessment
Objective: Assessment of ischemic infarct size in mice following stroke induction and evaluation of post-stroke recovery and angiogenic responses
This is a Stroke Model with Infarct Size Assessment protocol using mouse as the model organism. The procedure involves 5 procedural steps. Extracted from a 2011 paper published in Journal of Neuroscience.
Model and subjects
mouse • BDNF Met/Met knock-in mice and wild-type controls, with some CD36 knock-out crosses • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Stroke Induction • Infarct Size Assessment • Post-Stroke Locomotor Function Assessment
Primary readouts
- Infarct size
- CNS BDNF levels
- Post-stroke locomotor function
- Angiogenesis
Key equipment and reagents
Verified items
0
Direct vendor links
0
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Stroke Induction
Induce ischemic stroke in mice using unspecified method
Note: The methods section provided does not contain detailed stroke induction protocol
View evidence from paper
“investigate the effect of the BDNF genetic variant on ischemic outcome by using mice with a genetic knock-in”
Infarct Size Assessment
Assess ischemic infarct size in BDNF Met/Met mice compared with wild-type mice
Note: Specific methodology for infarct size measurement not detailed in provided text
View evidence from paper
“BDNF Met/Met mice exhibited reduced CNS BDNF levels without a discernable effect on infarct size”
Post-Stroke Locomotor Function Assessment
Evaluate post-stroke locomotor function deficits in BDNF Met/Met mice
Note: Specific behavioral testing methodology not detailed in provided text
View evidence from paper
“Diminished BDNF levels in BDNF Met/Met mice were associated with greater deficits in post-stroke locomotor functions”
Angiogenesis Assessment
Measure angiogenic response and expression of angiogenic/anti-angiogenic factors
Note: BDNF Met/Met mice showed reduced angiogenesis with elevated TSP-1 and CD36 expression
View evidence from paper
“BDNF Met/Met mice showed reduced angiogenesis and elevated expression of thrombospondin-1 (TSP-1) and its receptor CD36”
Double-Mutant Analysis
Cross BDNF Met/Met mice with CD36 knock-out mice and assess angiogenic rescue
Note: Double-mutant mice rescued angiogenic deficit without altering BDNF levels
View evidence from paper
“crossed BDNF Met/Met mice with CD36 knock-out mice. The double-mutant mice rescued the angiogenic deficit”