Source Paper
Genetic Variant of BDNF (Val66Met) Polymorphism Attenuates Stroke-Induced Angiogenic Responses by Enhancing Anti-Angiogenic Mediator CD36 Expression
Luye Qin, Eunhee Kim, Rajiv Ratan, Francis S. Lee, Sunghee Cho
Journal of Neuroscience • 2011
Stroke Model with Infarct Size Assessment
Objective: Assessment of ischemic infarct size in mice following stroke induction and evaluation of post-stroke recovery and angiogenic responses
Protocol Steps
Stroke Induction
Induce ischemic stroke in mice using unspecified method
Note: The methods section provided does not contain detailed stroke induction protocol
View evidence from paper
“investigate the effect of the BDNF genetic variant on ischemic outcome by using mice with a genetic knock-in”
Infarct Size Assessment
Assess ischemic infarct size in BDNF Met/Met mice compared with wild-type mice
Note: Specific methodology for infarct size measurement not detailed in provided text
View evidence from paper
“BDNF Met/Met mice exhibited reduced CNS BDNF levels without a discernable effect on infarct size”
Post-Stroke Locomotor Function Assessment
Evaluate post-stroke locomotor function deficits in BDNF Met/Met mice
Note: Specific behavioral testing methodology not detailed in provided text
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“Diminished BDNF levels in BDNF Met/Met mice were associated with greater deficits in post-stroke locomotor functions”
Angiogenesis Assessment
Measure angiogenic response and expression of angiogenic/anti-angiogenic factors
Note: BDNF Met/Met mice showed reduced angiogenesis with elevated TSP-1 and CD36 expression
View evidence from paper
“BDNF Met/Met mice showed reduced angiogenesis and elevated expression of thrombospondin-1 (TSP-1) and its receptor CD36”
Double-Mutant Analysis
Cross BDNF Met/Met mice with CD36 knock-out mice and assess angiogenic rescue
Note: Double-mutant mice rescued angiogenic deficit without altering BDNF levels
View evidence from paper
“crossed BDNF Met/Met mice with CD36 knock-out mice. The double-mutant mice rescued the angiogenic deficit”