Source Paper
Early Functional Deficit and Microglial Disturbances in a Mouse Model of Amyotrophic Lateral Sclerosis
Yannick Nicolas Gerber, Jean-Charles Sabourin, Miriam Rabano, Maria d M Vivanco, Florence Evelyne Perrin
PLoS ONE • 2012
View Abstract
In conclusion, precise motor analysis updates the onset of the disease in hSOD1(G93A) mice and allows locomotor monitoring until the end stage of the disease. Early functional deficits coincide with alterations of neuromuscular junctions. Importantly, we identify different sets of changes in microglia before disease onset as well as at early-symptomatic stage. This finding not only brings a new sequence of cellular events in the natural history of the disease, but it may also provide clues in the search for biomarkers of the disease, and potential therapeutic targets.
Weight Monitoring
Objective: Weekly measurement of body weight in transgenic mice from 56 days of age onward to monitor growth and health status
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Materials1
The Jackson Laboratory • RRID:IMSR_JAX:002298
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Protocol Steps
Animal housing and maintenance
House transgenic mice in controlled conditions with regulated hygrometry, temperature, and 12-hour light/dark cycle
Note: Only male mice were used; litter-matching between groups was done as much as possible
View evidence from paper
“Transgenic mice were identified by PCR and housed in controlled conditions (hygrometry, temperature and 12 h light/dark cycle). Only males were used and litter-matching between groups were done as much as possible.”
Weekly weight measurement
Measure body weight of each mouse once per week starting from 56 days of age
Note: Measurements continue from 56 days of age onward
View evidence from paper
“Record of the weight was done once a week from 56 days onward”