Neurological Deficit Assessment
Objective: Monitor neurological deficits in mice up to 21 days after stroke induction, and assess angiogenesis, neural plasticity, microglial polarization, and microglia-associated inflammatory cytokines in the peri-infarct cortex
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Materials1
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Protocol Steps
Ischemic Stroke Induction
Induce ischemic stroke in mice using permanent distal middle cerebral artery occlusion combined with 1 hour hypoxia
Note: This is the baseline procedure before neurological deficit monitoring begins
View evidence from paper
“Ischemic stroke mice model was induced by permanent distal middle cerebral artery occlusion plus 1 hour hypoxia”
TWS119 Administration
Administer TWS119 to stroke-induced mice starting from day 1 post-stroke
Note: Treatment period spans 14 days
View evidence from paper
“TWS119 was administrated from day 1 to 14 after stroke”
Neurological Deficit Monitoring
Monitor and assess neurological deficits in mice following stroke induction
Note: Monitoring continues throughout the entire observation period, including after TWS119 administration ends
View evidence from paper
“Neurological deficits were monitored up to 21 days after stroke”
Tissue Collection and Analysis at Day 14
Collect peri-infarct cortex tissue and analyze for angiogenesis, neural plasticity, microglial polarization, and microglia-associated inflammatory cytokines
Note: First timepoint for molecular analysis
View evidence from paper
“Angiogenesis, neural plasticity, microglial polarization, and microglia-associated inflammatory cytokines were detected in the peri-infarct cortex at days 14 and 21 after stroke”
Tissue Collection and Analysis at Day 21
Collect peri-infarct cortex tissue and analyze for angiogenesis, neural plasticity, microglial polarization, and microglia-associated inflammatory cytokines
Note: Second timepoint for molecular analysis, final observation timepoint
View evidence from paper
“Angiogenesis, neural plasticity, microglial polarization, and microglia-associated inflammatory cytokines were detected in the peri-infarct cortex at days 14 and 21 after stroke”
In Vitro Mechanistic Studies
Employ primary microglia and mouse brain microvascular endothelial cell lines to explore underlying mechanisms
Note: Complementary in vitro experiments to elucidate mechanisms observed in vivo
View evidence from paper
“Primary microglia and mouse brain microvascular endothelial cell lines were employed to explore the underlying mechanism in vitro”