Source Paper
Functional assessments in the rodent stroke model
Krystal L Schaar, Miranda M Brenneman, Sean I Savitz
Experimental & Translational Stroke Medicine • 2010
View Abstract
Abstract Stroke is a common cause of permanent disability accompanied by devastating impairments for which there is a pressing need for effective treatment. Motor, sensory and cognitive deficits are common following stroke, yet treatment is limited. Along with histological measures, functional outcome in animal models has provided valuable insight to the biological basis and potential rehabilitation efforts of experimental stroke. Developing and using tests that have the ability to identify behavioral deficits is essential to expanding the development of translational therapies. The present aim of this paper is to review many of the current behavioral tests that assess functional outcome after stoke in rodent models. While there is no perfect test, there are many assessments that are sensitive to detecting the array of impairments, from global to modality specific, after stroke.
Adhesive Removal Test
Objective: Assess tactile responses and sensorimotor asymmetries through removal of adhesive stimuli applied to rodent forelimbs
This is a Adhesive Removal Test protocol using rodent (rat or mouse) as the model organism. The procedure involves 3 procedural steps, 1 materials. Extracted from a 2010 paper published in Experimental & Translational Stroke Medicine.
Model and subjects
rodent (rat or mouse) • not specified • unknown • not specified • not specified
Study window
~5 day study window | ~10 minutes hands-on
Core workflow
Pre-test exposure (optional but recommended) • Apply adhesive stimuli • Conduct trials
Primary readouts
- Tactile responses to adhesive stimuli
- Sensorimotor asymmetries between forelimbs
- Time to remove adhesive
- Limb preference during removal attempts
Key equipment and reagents
Verified items
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Protocol Steps
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Pre-test exposure (optional but recommended)
Expose animals to pasta 5 days before testing for optimal performance
Note: This exposure is noted as best practice for optimal performance
View evidence from paper
“None (exposure to pasta 5 days before testing is best for optimal performance)”
Apply adhesive stimuli
Apply adhesive to forelimbs to assess tactile responses and sensorimotor asymmetries
Note: Test assesses both tactile responses and asymmetries between limbs
View evidence from paper
“Adhesive Removal Assesses tactile responses and asymmetries”
Conduct trials
Observe and record animal behavior during adhesive removal attempts
Note: Conduct 4-5 trials per session
View evidence from paper
“Adhesive Removal None (exposure to pasta 5 days Before testing is best for optimal performance) 4-5 trials 5-10 minutes”