Source Paper
Anxiolytic- and antidepressant-like effects of the non-peptide vasopressin V <sub>1b</sub> receptor antagonist, SSR149415, suggest an innovative approach for the treatment of stress-related disorders
Guy Griebel, Jacques Simiand, Claudine Serradeil-Le Gal, Jean Wagnon, Marc Pascal et al.
Proceedings of the National Academy of Sciences • 2002
Light/Dark Test
Objective: A classical anxiety test measuring rodent preference for dark versus illuminated compartments to assess anxiolytic-like effects
This is a Light/Dark Test protocol using rodent (rat or mouse) as the model organism. The procedure involves 2 procedural steps, 1 equipment items. Extracted from a 2002 paper published in Proceedings of the National Academy of Sciences.
Model and subjects
rodent (rat or mouse) • Not specified • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Animal Preparation • Light/Dark Test Execution
Primary readouts
- Preference for dark versus illuminated compartments
- Anxiolytic-like activity
- Time spent in each compartment (implied)
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Animal Preparation
Prepare rodents for light/dark test. Specific preparation details not provided in methods section.
Note: Test used to assess anxiolytic-like activity of SSR149415 at doses ranging from 1 to 30 mg/kg administered intraperitoneally or orally
View evidence from paper
“SSR149415 produced anxiolytic-like activity at doses that ranged from 1 to 30 mg/kg (i.p. or p.o.)”
Light/Dark Test Execution
Conduct light/dark test to measure anxiety-like behavior. Specific procedural details not provided in methods section.
Note: Test is classified as a classical anxiety model. Diazepam was used as positive control for anxiolytic effects.
View evidence from paper
“classical (punished drinking, elevated plus-maze, and light/dark tests) and atypical (fear/anxiety defense test battery and social defeat-induced anxiety) rodent models of anxiety”