Source Paper
Anxiolytic- and antidepressant-like effects of the non-peptide vasopressin V <sub>1b</sub> receptor antagonist, SSR149415, suggest an innovative approach for the treatment of stress-related disorders
Guy Griebel, Jacques Simiand, Claudine Serradeil-Le Gal, Jean Wagnon, Marc Pascal et al.
Proceedings of the National Academy of Sciences • 2002
Elevated Plus-Maze
Objective: Measure anxiolytic-like effects of SSR149415 in the elevated plus-maze, a classical rodent model of anxiety that assesses exploration of open versus closed arms
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified • Not specified • Not specified • Not specified
Materials2
Sanofi-Synthelabo Recherche • Not specified • Not specified • Not specified
Not specified • Not specified • Not specified • Not specified
Software1
Not specified • Not specified
1 item available from ConductScience
Estimated: $1,890.00
As an Amazon Associate, we earn from qualifying purchases. Product links help support this free resource.
Protocol Steps
Drug Administration
Administer SSR149415 at doses ranging from 1 to 30 mg/kg via intraperitoneal (i.p.) or oral (p.o.) routes
Note: Diazepam used as positive control at unspecified dose
View evidence from paper
“SSR149415 produced anxiolytic-like activity at doses that ranged from 1 to 30 mg/kg (i.p. or p.o.)”
Elevated Plus-Maze Testing
Place rodent in elevated plus-maze apparatus and measure exploration behavior of open versus closed arms as indicator of anxiety-like behavior
Note: This is a classical test of anxiety in rodents
View evidence from paper
“classical (punished drinking, elevated plus-maze, and light/dark tests) and atypical (fear/anxiety defense test battery”
Behavioral Observation
Observe and record rodent behavior in the elevated plus-maze to assess anxiolytic-like activity
Note: Magnitude of SSR149415 effects was overall less than diazepam in classical anxiety tests
View evidence from paper
“the magnitude of these effects was overall less than that of the benzodiazepine anxiolytic diazepam”