Source Paper
Anxiolytic- and antidepressant-like effects of the non-peptide vasopressin V <sub>1b</sub> receptor antagonist, SSR149415, suggest an innovative approach for the treatment of stress-related disorders
Guy Griebel, Jacques Simiand, Claudine Serradeil-Le Gal, Jean Wagnon, Marc Pascal et al.
Proceedings of the National Academy of Sciences • 2002
Elevated Plus-Maze
Objective: Measure anxiolytic-like effects of SSR149415 in the elevated plus-maze, a classical rodent model of anxiety that assesses exploration of open versus closed arms
This is a Elevated Plus-Maze protocol using rodent (rat or mouse) as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 2 materials. Extracted from a 2002 paper published in Proceedings of the National Academy of Sciences.
Model and subjects
rodent (rat or mouse) • Not specified • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Drug Administration • Elevated Plus-Maze Testing • Behavioral Observation
Primary readouts
- Exploration of open arms versus closed arms
- Anxiolytic-like activity
- Time spent in open versus closed arms
- Frequency of arm entries
Key equipment and reagents
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Protocol Steps
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Drug Administration
Administer SSR149415 at doses ranging from 1 to 30 mg/kg via intraperitoneal (i.p.) or oral (p.o.) routes
Note: Diazepam used as positive control at unspecified dose
View evidence from paper
“SSR149415 produced anxiolytic-like activity at doses that ranged from 1 to 30 mg/kg (i.p. or p.o.)”
Elevated Plus-Maze Testing
Place rodent in elevated plus-maze apparatus and measure exploration behavior of open versus closed arms as indicator of anxiety-like behavior
Note: This is a classical test of anxiety in rodents
View evidence from paper
“classical (punished drinking, elevated plus-maze, and light/dark tests) and atypical (fear/anxiety defense test battery”
Behavioral Observation
Observe and record rodent behavior in the elevated plus-maze to assess anxiolytic-like activity
Note: Magnitude of SSR149415 effects was overall less than diazepam in classical anxiety tests
View evidence from paper
“the magnitude of these effects was overall less than that of the benzodiazepine anxiolytic diazepam”