Source Paper
Fecal microbiota transplantation protects rotenone-induced Parkinson’s disease mice via suppressing inflammation mediated by the lipopolysaccharide-TLR4 signaling pathway through the microbiota-gut-brain axis
Zhe Zhao, Jingwen Ning, Xiu-qi Bao, Meiyu Shang, Jingwei Ma et al.
Microbiome • 2021
Adhesive Removal Test
Objective: Evaluation of sensorimotor function by measuring the time to remove adhesive from paws
Protocol Steps
Animal acclimation
Mice were acclimatized under standard conditions with 12-hour light/dark cycle, temperature 22±2°C, humidity 50-60%, with ad libitum access to food and water
Note: Humidity maintained at 50-60%
View evidence from paper
“The mice were then acclimatized (12-h light/dark cycle) under standard conditions (temperature 22 ± 2 °C, humidity 50–60%) with ad libitum access to food and water for 7 days”
Treatment administration
Control group received vehicle administration daily. Model group received oral rotenone administration daily for 4 weeks
Note: After 4 weeks, model group was divided into Rotenone group (n=15) and FMT group (n=15)
View evidence from paper
“In the beginning 4 weeks, the model group received the oral administration of rotenone every day. Meanwhile, the control group mice were administrated with vehicle”
Secondary treatment phase
FMT group received FMT treatment once per day. Control group and Rotenone group received vehicle administration once per day
Note: Mice were daily weighed throughout the 6-week period
View evidence from paper
“During week 5 and 6, the mice in the FMT group were treated with FMT once per day. In the meantime, the control group and the rotenone group mice received vehicle administration once a day”
Adhesive removal test
Behavioral test performed to measure sensorimotor function by assessing time required to remove adhesive from paws
Note: Test performed at week 6 of the study
View evidence from paper
“GI function tests and behavioral tests were performed at week 6. D Adhesive removal test”