Experiments/Adhesive Removal Test

Source Paper

Fecal microbiota transplantation protects rotenone-induced Parkinson’s disease mice via suppressing inflammation mediated by the lipopolysaccharide-TLR4 signaling pathway through the microbiota-gut-brain axis

Zhe Zhao, Jingwen Ning, Xiu-qi Bao, Meiyu Shang, Jingwei Ma et al.

Microbiome • 2021

DOI PMC

Adhesive Removal Test

behavioralmouseC57BL/6J

Objective: Evaluation of sensorimotor function by measuring the time to remove adhesive from paws

This is a Adhesive Removal Test protocol using mouse as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 1 materials. Extracted from a 2021 paper published in Microbiome.

Workflow actionsRequest QuoteOpen Source Paper

Jump to Vendor Comparison

Overview Experimental Context Protocol Vendor Comparison Materials Checklist Evidence / Verification Related Experiments

Replication Essentials

Model and subjects

mouse • C57BL/6J • male • 8 weeks • 20-22 g • 45

Study window

~4 week study window

Core workflow

Animal acclimation • Treatment administration • Secondary treatment phase

Primary readouts

Time to remove adhesive from paws

Key equipment and reagents

Testing apparatus for adhesive removal testAdhesive

Procurement Snapshot

Verified items

Direct vendor links

View vendor comparison Request Quote

Before You Start

Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.

Confirm first

Verify the animal model, intervention setup, and collection timepoints against the source paper.
Check that every direct vendor link matches the exact specification your lab plans to run.

Use the page like this

Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.

Jump to vendor comparison Jump to checklist Jump to methods evidence

Protocol Steps

Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.

Animal acclimation

Mice were acclimatized under standard conditions with 12-hour light/dark cycle, temperature 22±2°C, humidity 50-60%, with ad libitum access to food and water

7 days22 ± 2°C

Note: Humidity maintained at 50-60%

View evidence from paper

“The mice were then acclimatized (12-h light/dark cycle) under standard conditions (temperature 22 ± 2 °C, humidity 50–60%) with ad libitum access to food and water for 7 days”

Treatment administration

Control group received vehicle administration daily. Model group received oral rotenone administration daily for 4 weeks

4 weeks

Note: After 4 weeks, model group was divided into Rotenone group (n=15) and FMT group (n=15)

View evidence from paper

“In the beginning 4 weeks, the model group received the oral administration of rotenone every day. Meanwhile, the control group mice were administrated with vehicle”

Secondary treatment phase

FMT group received FMT treatment once per day. Control group and Rotenone group received vehicle administration once per day

2 weeks (weeks 5-6)

Note: Mice were daily weighed throughout the 6-week period

View evidence from paper

“During week 5 and 6, the mice in the FMT group were treated with FMT once per day. In the meantime, the control group and the rotenone group mice received vehicle administration once a day”