Amblyopia Restoration Assay
Objective: Assessment of binocular vision restoration in amblyopic mice following transient plasticity reopening via RK peptide infusion to disrupt Otx2 localization to perineuronal nets
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Materials4
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Protocol Steps
Induce amblyopia
Close one eye during critical period to reduce cortical acuity and create amblyopic condition
Note: This creates the baseline amblyopic state in mice
View evidence from paper
“The closure of one eye during this transient window reduces cortical acuity”
Reopen plasticity in adult mice
Directly infuse RK peptide to disrupt endogenous Otx2 localization to parvalbumin-expressing cells and reopen plasticity in adult amblyopic mice
Note: RK peptide is specific to this function; Alanine-Alanine variant and scrambled peptide controls do not reactivate plasticity
View evidence from paper
“Direct infusion of RK peptide similarly disrupts endogenous Otx2 localization to PV cells, reduces PV and PNN expression, and reopens plasticity in adult mice”
Assess binocular vision restoration
Evaluate restoration of binocular vision in amblyopic mice following transient plasticity reopening
Note: This transient reopening of plasticity in the adult restores binocular vision in amblyopic mice
View evidence from paper
“Conversely, this transient reopening of plasticity in the adult restores binocular vision in amblyopic mice”