Anhedonia Test
Objective: Measurement of anhedonic behavior (reduced pleasure-seeking) in offspring exposed to prenatal immune activation via Poly-I:C on gestation day 17
This is a Anhedonia Test protocol using mouse as the model organism. The procedure involves 3 procedural steps, 2 materials. Extracted from a 2010 paper published in Neuropsychopharmacology.
Model and subjects
mouse • Not specified in provided text • both • Not specified in provided text • Not specified in provided text
Study window
Estimated timing pending
Core workflow
Prenatal Immune Activation • Anhedonia Assessment • Acute Apomorphine Treatment
Primary readouts
- Anhedonic behavior (reduced pleasure-seeking)
- Social interaction deficits
- Locomotor responses to apomorphine
- Stereotyped behavioral responses to apomorphine
Key equipment and reagents
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Protocol Steps
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Prenatal Immune Activation
Pregnant dams receive intravenous injection of Poly-I:C on gestation day 17
Note: Dose: 5 mg/kg, i.v. route
View evidence from paper
“prenatal exposure to the viral mimic polyriboinosinic-polyribocytidilic acid (Poly-I:C; 5 mg/kg, i.v.) on gestation day (GD) 17”
Anhedonia Assessment
Measurement of anhedonic behavior in offspring born to immune-challenged mothers
Note: Anhedonic behavior was one of the significant deficits observed in offspring
View evidence from paper
“prenatal exposure to the viral mimic polyriboinosinic-polyribocytidilic acid (Poly-I:C; 5 mg/kg, i.v.) on gestation day (GD) 17 led to significant deficits in social interaction, anhedonic behavior”
Acute Apomorphine Treatment
Offspring receive acute apomorphine treatment to assess locomotor and stereotyped behavioral responses
Note: Assessment conducted in both male and female offspring
View evidence from paper
“alterations in the locomotor and stereotyped behavioral responses to acute apomorphine (APO) treatment in both male and female offspring”