Anhedonia Test
Objective: Measurement of anhedonic behavior (reduced pleasure-seeking) in offspring exposed to prenatal immune activation via Poly-I:C on gestation day 17
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Protocol Steps
Prenatal Immune Activation
Pregnant dams receive intravenous injection of Poly-I:C on gestation day 17
Note: Dose: 5 mg/kg, i.v. route
View evidence from paper
“prenatal exposure to the viral mimic polyriboinosinic-polyribocytidilic acid (Poly-I:C; 5 mg/kg, i.v.) on gestation day (GD) 17”
Anhedonia Assessment
Measurement of anhedonic behavior in offspring born to immune-challenged mothers
Note: Anhedonic behavior was one of the significant deficits observed in offspring
View evidence from paper
“prenatal exposure to the viral mimic polyriboinosinic-polyribocytidilic acid (Poly-I:C; 5 mg/kg, i.v.) on gestation day (GD) 17 led to significant deficits in social interaction, anhedonic behavior”
Acute Apomorphine Treatment
Offspring receive acute apomorphine treatment to assess locomotor and stereotyped behavioral responses
Note: Assessment conducted in both male and female offspring
View evidence from paper
“alterations in the locomotor and stereotyped behavioral responses to acute apomorphine (APO) treatment in both male and female offspring”