Animal Housing and Husbandry
Objective: Housing and maintenance of female C57BL/6 mice under controlled environmental conditions for experimental use
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
1 item available from ConductScience
Estimated: $9,900.00
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Protocol Steps
Acquire mice
Purchase female C57BL/6 mice aged 6-8 weeks from vendor
Note: Mice obtained from Nanjing Pengsheng Biological Technology Co.
View evidence from paper
“Female C57BL/6 mice (6–8 weeks) were purchased from Nanjing Pengsheng Biological Technology Co.”
House mice in cages
Place mice in individually ventilated cages at a density of five mice per cage
Note: Maintain group housing with five mice per cage
View evidence from paper
“Mice were housed in individually ventilated cages with five mice per cage”
Establish light-dark cycle
Maintain a regular 12-hour light and 12-hour dark cycle with lights on from 8:00 AM to 8:00 PM and lights off from 8:00 PM to 8:00 AM
Note: Consistent daily schedule for circadian rhythm maintenance
View evidence from paper
“kept on a regular 12-h:12-h light:dark cycle (8:00 AM–8:00 PM light; 8:00 PM–8:00 AM dark)”
Control temperature
Maintain ambient temperature at 21°C with a tolerance of ±1°C
Note: Precise temperature control required for consistent experimental conditions
View evidence from paper
“with controlled temperature (21 ± 1°C)”
Control humidity
Maintain relative humidity between 40% and 70%
Note: Humidity range must be maintained throughout housing period
View evidence from paper
“and humidity (40–70%)”
Provide food and water
Supply food and water ad libitum to all mice
Note: Unrestricted access to both food and water
View evidence from paper
“Food and water were supplied ad libitum”