Anxiety-Related Behavior Testing
Objective: Assessment of baseline and stress-induced anxiety-related behaviors in mice using an inducible transgenic mouse model with increased adult hippocampal neurogenesis
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Materials1
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Protocol Steps
Baseline anxiety and depression-related behavior assessment
Assess baseline anxiety and depression-related behaviors in mice prior to stress induction
Note: No effects on baseline anxiety and depression-related behavior were found in the transgenic model
View evidence from paper
“We find no effects on baseline anxiety and depression-related behavior”
Chronic corticosterone administration
Treat mice chronically with corticosterone to induce stress model
Note: This creates a chronic stress model for testing anxiety and depression-related behaviors
View evidence from paper
“mice treated chronically with corticosterone (CORT), a mouse model of stress”
Stress-induced anxiety and depression-related behavior assessment
Assess anxiety and depression-related behaviors following chronic corticosterone treatment
Note: Increasing adult neurogenesis was sufficient to reduce anxiety and depression-related behaviors under chronic stress conditions
View evidence from paper
“we find that increasing adult neurogenesis is sufficient to reduce anxiety and depression-related behaviors in mice treated chronically with corticosterone”
HPA axis regulation assessment
Evaluate hypothalamic-pituitary-adrenal (HPA) axis regulation at baseline and following chronic CORT administration
Note: No effect of increased adult hippocampal neurogenesis on HPA axis regulation was found
View evidence from paper
“we find no effect of increased adult hippocampal neurogenesis on hypothalamic–pituitary–adrenal (HPA) axis regulation, either at baseline or following chronic CORT administration”