Anxiety-Related Behavior Assessment
Objective: Evaluation of anxiety-related behaviors in heterozygous mice with Na,K-ATPase α isoform gene deficiencies
This is a Anxiety-Related Behavior Assessment protocol using mouse as the model organism. The procedure involves 4 procedural steps, 1 equipment items. Extracted from a 2007 paper published in Journal of Neuroscience.
Model and subjects
mouse • Not explicitly specified in provided text • unknown • Not explicitly specified in provided text • Not explicitly specified in provided text
Study window
Estimated timing pending
Core workflow
Anxiety-related behavior assessment • Spatial learning assessment in Morris water maze • Locomotor activity assessment
Primary readouts
- Anxiety-related behavior
- Locomotor activity
- Spatial learning performance
- Memory deficits
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Anxiety-related behavior assessment
Evaluation of anxiety-related behaviors in heterozygous mice with Na,K-ATPase α isoform gene deficiencies
Note: Mice heterozygous for α2 isoform displayed increased anxiety-related behavior
View evidence from paper
“mice heterozygous for the α2 isoform display increased anxiety-related behavior, reduced locomotor activity, and impaired spatial learning in the Morris water maze”
Spatial learning assessment in Morris water maze
Assessment of spatial learning and memory deficits in heterozygous mice
Note: α2 heterozygous mice showed impaired spatial learning; α3 heterozygous mice displayed spatial learning and memory deficits unrelated to cued learning differences
View evidence from paper
“Mice heterozygous for the α3 isoform displayed spatial learning and memory deficits unrelated to differences in cued learning in the Morris maze”
Locomotor activity assessment
Measurement of locomotor activity levels in heterozygous mice
Note: α2 heterozygous mice showed reduced locomotor activity; α3 heterozygous mice showed increased locomotor activity
View evidence from paper
“mice heterozygous for the α2 isoform display increased anxiety-related behavior, reduced locomotor activity, and impaired spatial learning”
Methamphetamine response assessment
Evaluation of locomotor response to methamphetamine administration
Note: Both α3 and α1 heterozygous mice showed increased locomotor response to methamphetamine
View evidence from paper
“Mice heterozygous for the α3 isoform displayed spatial learning and memory deficits unrelated to differences in cued learning in the Morris maze, increased locomotor activity, an increased locomotor response to methamphetamine”