Aspiration Lesion
Objective: Induction of aspiration lesions in cortex as a control to compare axonal sprouting response with thermal-ischemic lesions
This is a Aspiration Lesion protocol using Not specified in provided text as the model organism. The procedure involves 4 procedural steps, 2 equipment items, 1 materials. Extracted from a 2002 paper published in Journal of Neuroscience.
Model and subjects
Not specified in provided text • Not specified in provided text • unknown • Adult • Not specified in provided text
Study window
Estimated timing pending
Core workflow
Induce aspiration lesion in sensorimotor cortex • Record neuronal activity on day 1 post-lesion • Record neuronal activity on days 2-3 post-lesion
Primary readouts
- Presence or absence of synchronized neuronal activity patterns
- Frequency range of neuronal activity (0.2-2 Hz early, 0.1-0.4 Hz later)
- Degree of axonal sprouting from contralateral corticostriatal neurons
- Comparison of sprouting response between aspiration and thermal-ischemic lesions
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Induce aspiration lesion in sensorimotor cortex
Create similarly sized aspiration lesions in sensorimotor cortex as control lesions to compare with thermal-ischemic lesions
Note: Aspiration lesions do not induce axonal sprouting, unlike thermal-ischemic lesions
View evidence from paper
“similarly sized aspiration lesions, which do not induce axonal sprouting”
Record neuronal activity on day 1 post-lesion
Monitor and record synchronized neuronal activity in perilesion cortex on day 1 after aspiration lesion
Note: Aspiration lesions do not produce the low-frequency synchronized neuronal activity seen with thermal-ischemic lesions
View evidence from paper
“An early rhythm of synchronous neuronal activity occurred in perilesion cortex on day 1 after lesion, with a frequency range of 0.2–2 Hz”
Record neuronal activity on days 2-3 post-lesion
Monitor and record neuronal activity patterns in cortical areas on days 2 and 3 after aspiration lesion
Note: Aspiration lesions do not produce the later pattern of synchronized activity seen with thermal-ischemic lesions
View evidence from paper
“A later pattern of activity occurred on days 2 and 3 after lesion, with a frequency range of 0.1–0.4 Hz”
Compare axonal sprouting response
Assess and compare the degree of axonal sprouting from contralateral homotypic cortex after aspiration lesions versus thermal-ischemic lesions
Note: Aspiration lesions serve as control showing no axonal sprouting response
View evidence from paper
“take advantage of marked differences in the degree of axonal sprouting from contralateral homotypic cortex after two types of cortical lesions”