HIV Antigen Vaccination with Escalating Dosing Profiles
Objective: Compare traditional bolus vaccine dosing versus repeated injections or osmotic pump delivery of HIV antigens over 1-2 weeks to measure humoral immune responses and antibody production
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Equipment1
Not specified • Not specified • Not specified • Not specified
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Protocol Steps
Bolus vaccination
Traditional single-dose prime-boost vaccination method with complete antigen and adjuvant dose administered at once
Note: Control condition for comparison
View evidence from paper
“traditional single-dose prime–boost method”
Repeated injection dosing
Administration of HIV antigens and adjuvant through multiple repeated injections over 1-2 weeks
Note: Total antigen and adjuvant dose equivalent to bolus group
View evidence from paper
“administering a given total dose of antigen and adjuvant over 1–2 wk through repeated injections”
Osmotic pump delivery
Sustained antigen and adjuvant delivery via osmotic pump over 1-2 weeks
Note: Total antigen and adjuvant dose equivalent to bolus group
View evidence from paper
“administering a given total dose of antigen and adjuvant over 1–2 wk through repeated injections or osmotic pumps”
Exponentially increasing dosing profile
Antigen and adjuvant administered with exponentially increasing dose escalation over vaccination period
Note: Elicited >10-fold increases in antibody production relative to bolus vaccination
View evidence from paper
“exponentially increasing (exp-inc) dosing profiles eliciting >10-fold increases in antibody production relative to bolus vaccination post prime”
Measure humoral immune responses
Assess antibody titers and production following vaccination with different dosing profiles
Note: Primary outcome measure
View evidence from paper
“certain dosing profiles demonstrate >10-fold higher antibody production than the traditional single-dose prime–boost method”
Assess antigen retention in lymph nodes
Evaluate prolonged antigen availability in lymph nodes following different dosing regimens
Note: Consistent with computational predictions
View evidence from paper
“we found that exp-inc dosing led to prolonged antigen retention in lymph nodes”
Quantify germinal center response
Measure Tfh cell and germinal center B-cell numbers following vaccination
Note: Mechanistic assessment of immune response
View evidence from paper
“increased Tfh cell and germinal center B-cell numbers”