Source Paper
Hyperactivity and Intact Hippocampus-Dependent Learning in Mice Lacking the M<sub>1</sub>Muscarinic Acetylcholine Receptor
Tsuyoshi Miyakawa, Masahisa Yamada, Alokesh Duttaroy, Jürgen Wess
Journal of Neuroscience • 2001
Auditory-Cued Fear Conditioning
Objective: Evaluation of amygdala-dependent associative learning through auditory cue-shock pairing in M1 receptor-deficient mice
This is a Auditory-Cued Fear Conditioning protocol using mouse as the model organism. The procedure involves 1 procedural steps, 1 equipment items. Extracted from a 2001 paper published in Journal of Neuroscience.
Model and subjects
mouse • M1 receptor-deficient mice (M1R−/− mice) • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Auditory-Cued Fear Conditioning
Primary readouts
- Freezing behavior in response to auditory cues paired with footshocks
- Performance deficits in auditory-cued fear conditioning task
Key equipment and reagents
Verified items
0
Direct vendor links
0
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Auditory-Cued Fear Conditioning
Mice were subjected to auditory-cued fear conditioning as part of a behavioral test battery to assess amygdala-dependent associative learning
Note: M1R−/− mice showed slight performance deficits in this task, likely due to their hyperactivity phenotype rather than cognitive impairment
View evidence from paper
“M1R−/− mice showed slight performance deficits in auditory-cued fear conditioning and in an eight-arm radial maze, most likely because of the hyperactivity phenotype”