Source Paper
Atrophin-1 antisense oligonucleotide provides robust protection from pathology in a fully humanized DRPLA model
Smith VL, Gidi BZ, Bragg RM, Cantle JP, Ben-Varon A et al.
Mol Ther Nucleic Acids • 2025
Balance Beam
Objective: To assess motor coordination and balance ability in mice using a balance beam task
This is a Balance Beam protocol using Mouse as the model organism. The procedure involves 6 procedural steps, 1 equipment items, 6 materials. Extracted from a 2025 paper published in Mol Ther Nucleic Acids.
Model and subjects
Mouse • Not specified • Male and female • 6 to 7 weeks • Not specified
Study window
Estimated timing pending
Core workflow
Setup apparatus • Prepare dark box • Training day 1
Primary readouts
- Motor coordination and balance performance on balance beam task
Key equipment and reagents
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Protocol Steps
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Setup apparatus
Set up metal rod at tapered angle with dark box at one end and 60-watt lamp at the other end
View evidence from paper
“metal rod set up at a tapered angle with a dark box at one end and 60-watt lamp on the other”
Prepare dark box
Place nestling and bedding from the mouse's home cage in the dark box
View evidence from paper
“The dark box had nestling and bedding from the mouse's home cage”
Training day 1
Train mice with three trials on both the 12 mm and 6 mm diameter rods
View evidence from paper
“Mice were trained for two days with three trials on both rods”
Training day 2
Continue training mice with three trials on both the 12 mm and 6 mm diameter rods
View evidence from paper
“Mice were trained for two days with three trials on both rods”
Testing day
Test mice on the third day with three trials on the 6 mm diameter rod only
View evidence from paper
“The mice were tested on the third day with three trials on the 6 mm rod”
Clean between subjects
Clean the dark box and rod with 70% ethanol between different cages of mice
View evidence from paper
“The dark box and rod were cleaned with 70% ethanol between cages”