BAM8-22 Agonist-induced Itch Testing
Objective: Evaluation of itch induction following activation of MrgprC11-expressing neurons with BAM8-22 agonist in wild-type and mutant mice to determine if MrgprC11 activation is sufficient to induce itch behavior
This is a BAM8-22 Agonist-induced Itch Testing protocol using mouse as the model organism. The procedure involves 3 procedural steps, 1 materials. Extracted from a 2009 paper published in Cell.
Model and subjects
mouse • wild-type and mutant mice (specific strains not detailed in provided text) • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
BAM8-22 Administration • Itch Behavior Observation • Mutant Mouse Control
Primary readouts
- Itch-related behaviors in wild-type mice following BAM8-22 activation
- Absence of itch response in mutant mice lacking Mrgpr genes
- Comparison of itch induction between wild-type and mutant animals
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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BAM8-22 Administration
Administer MrgprC11-specific agonist BAM8-22 to activate MrgprC11-expressing neurons in test animals
Note: Administration method (injection, topical, etc.) not specified in provided text
View evidence from paper
“Activation of these neurons with MrgprC11-specific agonist BAM8-22 induces itch in wild-type but not mutant mice”
Itch Behavior Observation
Observe and record itch-related behaviors in wild-type mice following BAM8-22 administration
Note: Specific behavioral measures and observation duration not detailed in provided text
View evidence from paper
“Activation of these neurons with MrgprC11-specific agonist BAM8-22 induces itch in wild-type but not mutant mice”
Mutant Mouse Control
Perform identical BAM8-22 administration and observation in mutant mice lacking Mrgpr genes as negative control
Note: Mutant mice should show no itch response to BAM8-22
View evidence from paper
“Activation of these neurons with MrgprC11-specific agonist BAM8-22 induces itch in wild-type but not mutant mice”