Experiments/Baseline Anxiety Assessment

Source Paper

Hippocampus-specific deletion of BDNF in adult mice impairs spatial memory and extinction of aversive memories

S A Heldt, L Stanek, J P Chhatwal, K J Ressler

Molecular Psychiatry • 2007

DOI PMC

Baseline Anxiety Assessment

behavioralmouseNot specified

Objective: Measurement of baseline anxiety levels using acoustic startle response in adult mice with hippocampal-specific BDNF deletion

This is a Baseline Anxiety Assessment protocol using mouse as the model organism. The procedure involves 2 procedural steps, 1 equipment items, 2 materials. Extracted from a 2007 paper published in Molecular Psychiatry.

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Replication Essentials

Model and subjects

mouse • Not specified • Not specified • adult • Not specified • Not specified

Study window

Estimated timing pending

Core workflow

Lentivirus injection into dorsal hippocampus • Baseline anxiety assessment using startle response

Primary readouts

Baseline anxiety levels measured by acoustic startle response
Locomotion
Comparison between lenti-Cre-infected and lenti-GFP-infected control animals

Key equipment and reagents

Startle response measurement apparatusLentivirus expressing Cre recombinase

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Protocol Steps

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Lentivirus injection into dorsal hippocampus

Bilateral injection of lentivirus expressing Cre recombinase into the dorsal hippocampus of adult mice floxed at the BDNF locus

Not specifiedNot specified

Note: Performed in adult animals to achieve site-specific BDNF deletion

View evidence from paper

“we injected a lentivirus expressing Cre recombinase bilaterally into the dorsal hippocampus in adult mice floxed at the BDNF locus”