Baseline Anxiety Assessment
Objective: Measurement of baseline anxiety levels using acoustic startle response in adult mice with hippocampal-specific BDNF deletion
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
Not specified • Not specified • Not specified • Not mentioned
Materials2
Not specified • Not specified • Not specified • Not mentioned
Not specified • Not specified • Not specified • Not mentioned
1 item available from ConductScience
As an Amazon Associate, we earn from qualifying purchases. Product links help support this free resource.
Protocol Steps
Lentivirus injection into dorsal hippocampus
Bilateral injection of lentivirus expressing Cre recombinase into the dorsal hippocampus of adult mice floxed at the BDNF locus
Note: Performed in adult animals to achieve site-specific BDNF deletion
View evidence from paper
“we injected a lentivirus expressing Cre recombinase bilaterally into the dorsal hippocampus in adult mice floxed at the BDNF locus”
Baseline anxiety assessment using startle response
Measurement of baseline anxiety levels using acoustic startle response in both lenti-Cre-infected and control lenti-GFP-infected animals
Note: No significant effects of BDNF deletion on baseline anxiety were observed
View evidence from paper
“there were no significant effects of BDNF deletion on locomotion or baseline anxiety measured with startle”