BDNF Heterozygous Mice Phenotyping
Objective: Assessment of eating behavior and locomotor activity abnormalities in heterozygous BDNF mutant mice to determine BDNF's role in regulating eating behavior and locomotor activity
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Protocol Steps
Phenotypic Assessment of Heterozygous BDNF Mice
Evaluate heterozygous BDNF mutant mice for abnormalities in eating behavior and locomotor activity
Note: All heterozygous mice exhibited abnormalities in eating behavior or locomotor activity
View evidence from paper
“In heterozygous mice, BDNF gene expression is reduced and we find that all heterozygous mice exhibit abnormalities in eating behavior or locomotor activity”
Validation in Multiple Strains
Confirm phenotype in independently derived inbred and hybrid BDNF mutant strains
Note: Phenotype was observed across multiple independently derived strains
View evidence from paper
“We also observe this phenotype in independently derived inbred and hybrid BDNF mutant strains”
Neurotrophin Infusion Treatment
Infuse BDNF or NT4/5 to assess reversal of eating behavior abnormalities and obesity
Note: Treatment produced transient reversal of phenotype
View evidence from paper
“Infusion with BDNF or NT4/5 can transiently reverse the eating behavior and obesity”