Behavioral Assays
Objective: Performance assessment on behavioral assays requiring motor coordination to correlate with tau pathology in brain regions of P301L tangle model mice
This is a Behavioral Assays protocol using mouse as the model organism. The procedure involves 3 procedural steps, 3 materials. Extracted from a 2007 paper published in Journal of Neuroscience.
Model and subjects
mouse • P301L tangle model mice • unknown • Not specified • Not specified
Study window
Estimated timing pending
Core workflow
Behavioral Assay Performance Assessment • Antibody Injection into Carotid Artery • Brain Section Analysis
Primary readouts
- Performance on behavioral assays requiring motor coordination
- Tau pathology in corresponding brain areas
- Antibody levels against immunogen
- Presence of FITC-tagged antibodies in brain
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Behavioral Assay Performance Assessment
Conduct behavioral assays that require extensive motor coordination on P301L tangle model mice to assess performance
Note: Performance on these assays correlated with tau pathology in corresponding brain areas
View evidence from paper
“Performance on behavioral assays that require extensive motor coordination correlated with tau pathology in corresponding brain areas”
Antibody Injection into Carotid Artery
Inject FITC-tagged antibodies purified from P301L mouse with high antibody titer into the carotid artery of P301L mice to confirm antibodies could enter brain and bind to pathological tau
Note: Antibodies were subsequently detected within the brain and colocalized with PHF1 and MC1 antibodies
View evidence from paper
“FITC-tagged antibodies purified from a P301L mouse, with a high antibody titer against the immunogen, were injected into the carotid artery of P301L mice”
Brain Section Analysis
Analyze brain sections to detect tau antibodies and confirm colocalization with pathological tau markers
Note: Tau antibodies generated in animals recognized pathological tau on brain sections
View evidence from paper
“The tau antibodies generated in these animals recognized pathological tau on brain sections”