Behavioral Assays
Objective: Performance assessment on behavioral assays requiring motor coordination to correlate with tau pathology in brain regions of P301L tangle model mice
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Protocol Steps
Behavioral Assay Performance Assessment
Conduct behavioral assays that require extensive motor coordination on P301L tangle model mice to assess performance
Note: Performance on these assays correlated with tau pathology in corresponding brain areas
View evidence from paper
“Performance on behavioral assays that require extensive motor coordination correlated with tau pathology in corresponding brain areas”
Antibody Injection into Carotid Artery
Inject FITC-tagged antibodies purified from P301L mouse with high antibody titer into the carotid artery of P301L mice to confirm antibodies could enter brain and bind to pathological tau
Note: Antibodies were subsequently detected within the brain and colocalized with PHF1 and MC1 antibodies
View evidence from paper
“FITC-tagged antibodies purified from a P301L mouse, with a high antibody titer against the immunogen, were injected into the carotid artery of P301L mice”
Brain Section Analysis
Analyze brain sections to detect tau antibodies and confirm colocalization with pathological tau markers
Note: Tau antibodies generated in animals recognized pathological tau on brain sections
View evidence from paper
“The tau antibodies generated in these animals recognized pathological tau on brain sections”