Behavioral Experiments
Objective: Behavioral testing of control-fed and rapamycin-fed transgenic and non-transgenic PDAPP mice to assess behavioral differences
This is a Behavioral Experiments protocol using mouse as the model organism. The procedure involves 3 procedural steps, 2 materials. Extracted from a 2010 paper published in PLoS ONE.
Model and subjects
mouse • PDAPP [hAPP(J20)] mice crossed with C57BL/6J • male • 7 months • 44
Study window
~13 week study window
Core workflow
Establish experimental groups • Administer rapamycin treatment • Conduct behavioral experiments
Primary readouts
- Behavioral differences between control-fed and rapamycin-fed mice
- Behavioral differences between transgenic and non-transgenic mice
- Interaction effects between genotype and diet on behavior
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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Protocol Steps
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Establish experimental groups
Divide mice into four experimental groups based on genotype (transgenic vs non-transgenic) and diet (control-fed vs rapamycin-fed)
Note: All animals were males and 7 months old at time of testing
View evidence from paper
“Experimental groups were: control-fed non-Tg, n=10; rapamycin-fed non-Tg, n=10; control-fed Tg, n=12; rapamycin-fed Tg, n=12, all animals were males and 7 month-old at the time of testing.”
Administer rapamycin treatment
Administer rapamycin to designated experimental groups starting at 4 months of age
Note: Treatment begins at 4 months of age and continues for 13 weeks, concluding before behavioral testing at 7 months
View evidence from paper
“Rapamycin was administered for 13 weeks starting at 4 months of age.”
Conduct behavioral experiments
Perform behavioral testing on all four experimental groups at 7 months of age
Note: Specific behavioral tests are not detailed in the provided methods text
View evidence from paper
“Rapamycin administration and behavioral experiments involving PDAPP mice were conducted at the Buck Institute for Age Research, Novato, CA.”