Behavioral Experiments
Objective: Behavioral testing of control-fed and rapamycin-fed transgenic and non-transgenic PDAPP mice to assess behavioral differences
Gather these items before starting the experiment. Check off items as you prepare.
Materials2
Jackson Laboratories
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Protocol Steps
Establish experimental groups
Divide mice into four experimental groups based on genotype (transgenic vs non-transgenic) and diet (control-fed vs rapamycin-fed)
Note: All animals were males and 7 months old at time of testing
View evidence from paper
“Experimental groups were: control-fed non-Tg, n=10; rapamycin-fed non-Tg, n=10; control-fed Tg, n=12; rapamycin-fed Tg, n=12, all animals were males and 7 month-old at the time of testing.”
Administer rapamycin treatment
Administer rapamycin to designated experimental groups starting at 4 months of age
Note: Treatment begins at 4 months of age and continues for 13 weeks, concluding before behavioral testing at 7 months
View evidence from paper
“Rapamycin was administered for 13 weeks starting at 4 months of age.”
Conduct behavioral experiments
Perform behavioral testing on all four experimental groups at 7 months of age
Note: Specific behavioral tests are not detailed in the provided methods text
View evidence from paper
“Rapamycin administration and behavioral experiments involving PDAPP mice were conducted at the Buck Institute for Age Research, Novato, CA.”