Behavioral Sensitivity Testing
Objective: Test F1 and F2 generations for increased behavioral sensitivity to the F0-conditioned odor (acetophenone) compared to other odors
This is a Behavioral Sensitivity Testing protocol using mouse as the model organism. The procedure involves 2 procedural steps, 1 materials. Extracted from a 2013 paper published in Nature Neuroscience.
Model and subjects
mouse • Not specified in provided text • unknown • Not specified in provided text • Not specified in provided text
Study window
Estimated timing pending
Core workflow
F0 Odor Fear Conditioning • F1 and F2 Behavioral Sensitivity Testing
Primary readouts
- Behavioral sensitivity to acetophenone (F0-conditioned odor)
- Behavioral sensitivity to other odors (non-conditioned controls)
- Transgenerational inheritance of behavioral sensitivity
Key equipment and reagents
Verified items
0
Direct vendor links
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Protocol Steps
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F0 Odor Fear Conditioning
F0 mice were subjected to odor fear conditioning using acetophenone before conception
Note: Conditioning occurred prior to conception of F1 generation
View evidence from paper
“We subjected F0 mice to odor fear conditioning before conception”
F1 and F2 Behavioral Sensitivity Testing
F1 and F2 generations were tested for increased behavioral sensitivity to the F0-conditioned odor (acetophenone) compared to other odors
Note: Testing measured behavioral response specificity to acetophenone versus other odors
View evidence from paper
“subsequently conceived F1 and F2 generations had an increased behavioral sensitivity to the F0-conditioned odor, but not to other odors”